We narrowed to 9,894 results for: UTY

1. pFN33K LgBiT TK-neo Human collagen type IV alpha 5 chain (COL4A5)

   Plasmid

   #229768

   Purpose

   Human Col4A5 tagged at N-terminal with LgBiT to be used with N-tagged SmBiT hCol4A3 and Flag-tagged hCol4A4 in Nanobit assay system

   Depositor

   Hirofumi Kai

   Article

   Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

   Insert

   Human collagen type IV alpha 5 chain (COL4A5) (COL4A5 Human)
   

   Use

   Luciferase

   Tags

   LgBiT

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-U6-racRNA-A

   Plasmid

   #200824

   Purpose

   AAV transfer plasmid encoding a circularized RNA barcode A under U6 promoter and a subcellular localization protein binder under hSyn promoter

   Depositor

   Xiao Wang

   Article

   Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

   Inserts

   U6-racRNA
   V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
   

   Use

   AAV

   Tags

   C-Far, Flag, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-4T1 human PP2A B56α full length

   Plasmid

   #132635

   Purpose

   expresses human PP2A B56α full length in E. coli

   Depositor

   Wenqing Xu

   Article

   Sangodkar et al J Clin Invest. 2017 Jun 1;127(6):2081-20

   Insert

   PP2A B56α
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDRM55 tet-AR(1-707)

   Plasmid

   #183503

   Purpose

   Lentiviral vector expressing a doxycycline-inducible constitutively active truncated androgen receptor (wild type AR)

   Depositor

   Wayne Tilley

   Article

   Hickey et al Nat Med. 2021 Feb;27(2):310-320. doi: 10

   Insert

   Androgen receptor (AR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Constitutively active truncated AR; spans AR 1-70…

   Promoter

   CMV

   Available Since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFN35K SmBiT TK-neo Human collagen type IV alpha 3 chain (COL4A3)

   Plasmid

   #229767

   Purpose

   Human Col4A3 tagged at N-terminal with SmBiT to be used with N-tagged LgBiT hCol4A5 and Flag-tagged hCol4A4 in Nanobit assay system

   Depositor

   Hirofumi Kai

   Article

   Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

   Insert

   Human collagen type IV alpha 3 chain (COL4A3) (COL4A3 Human)
   

   Use

   Luciferase

   Tags

   SmBiT

   Expression

   Mutation

   See Depositor Comments

   Promoter

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-FLEx-iSeroSnFR-EnhancedExport

   Plasmid

   #129180

   Purpose

   Fluorescent reporter for serotonin imaging (conditional + membrane localization tag with enhanced export sequence)

   Depositor

   Lin Tian

   Article

   Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

   Insert

   iSeroSnFR
   

   Use

   AAV

   Tags

   Ig-kappa leader, Myc, and PDGFR + enhanced ER exp…

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS2 (CK2alpha', CK2beta)

   Plasmid

   #27093

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Inserts

   CK2beta (CSNK2B Human)
   CK2alpha' (CSNK2A2 Human)
   

   Use

   Tetracycline-regulated expression

   Tags

   HA and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   bidirectional tet-responsive promoter

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGV13 (CK2alpha K68M, CK2beta)

   Plasmid

   #27094

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Inserts

   CK2beta (CSNK2B Human)
   CK2alpha K68M (CSNK2A1 Human)
   

   Use

   Tetracycline-regulated expression

   Tags

   HA on CK2alpha and Myc on CK2beta

   Expression

   Mammalian

   Mutation

   K68M on CK2alpha

   Promoter

   bidirectional tet-responsive promoter and bidirec…

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPaint-mNeon-F-mgp100-PuroR [M1G]

   Plasmid

   #171802

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-FLAG-mgp100-T2A-PuroR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Promoter

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. JDW 742 (pDONR221-EGFP-KRAS4B-G12V)

    Plasmid

    #156403

    Purpose

    Gateway middle entry clone encoding EGFP-fused to human KRAS4B with G12V mutation, includes a stop codon

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4B-G12V (KRAS Human)
    

    Use

    Gateway cloning

    Tags

    EGFP

    Expression

    Mutation

    mutant KRAS4B-G12V

    Promoter

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHis6-SUMO-g0s2

    Plasmid

    #179293

    Purpose

    E. coli expression plasmid (T7 promoter) for full-length human G0S2 with N-terminal His6 + SUMO + TEV protease cleavage site

    Depositor

    Debra Hansen

    Article

    Moran et al PLoS One. 2021 Jul 14;16(7):e0249164. do

    Insert

    G0S2 (G0S2 Human)
    

    Use

    Tags

    His6 + SUMO + TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    full-length G0S2, residues 1-103 from accession n…

    Promoter

    T7

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. JDW 837 (pCS2-V5-mScarlet-I-hsKRAS4A-G12V)

    Plasmid

    #156410

    Purpose

    pCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4A-G12V

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4A-G12V (KRAS Human)
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    mutant KRAS4A-G12V

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. JDW 827 (pCS2-V5-mTagBFP2-hsKRAS4A-G12D)

    Plasmid

    #156409

    Purpose

    pCS2 CMV expression vector driving V5-tagged mTagBFP2 fused human KRAS4A-G12D

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4A-G12D (KRAS Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    mutant KRAS4A-G12D

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. JDW 829 (pTol2-kdrl:V5-mTagBFP2-hsKRAS4A-G12D-ac/Y)

    Plasmid

    #156415

    Purpose

    kdrl promoter driving V5-tagged mTagBFP2 fused to human KRAS4A-G12D with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4A-G12D (KRAS Human)
    

    Use

    Zebrafish transgenesis

    Tags

    mTagBFP2

    Expression

    Mutation

    mutant KRAS4A-G12D

    Promoter

    kdrl

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGV7 (CK2alpha' K69M, CK2beta)

    Plasmid

    #27095

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Inserts

    CK2beta (CSNK2B Human)
    CK2alpha' K69M (CSNK2A2 Human)
    

    Use

    Tetracycline-regulated expression

    Tags

    HA tag on CK2alpha' and Myc on CK2beta

    Expression

    Mammalian

    Mutation

    K69M on CK2alpha'

    Promoter

    bidirectional tet-responsive promoter

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-U6-racRNA-B

    Plasmid

    #200825

    Purpose

    AAV transfer plasmid encoding a circularized RNA barcode B under U6 promoter and a subcellular localization protein binder under hSyn promoter

    Depositor

    Xiao Wang

    Article

    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

    Inserts

    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    

    Use

    AAV

    Tags

    C-Far, Flag, and V5

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-U6-racRNA-C

    Plasmid

    #200826

    Purpose

    AAV transfer plasmid encoding a circularized RNA barcode C under U6 promoter and a subcellular localization protein binder under hSyn promoter

    Depositor

    Xiao Wang

    Article

    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

    Inserts

    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    

    Use

    AAV

    Tags

    C-Far, Flag, and V5

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-U6-racRNA-D

    Plasmid

    #200827

    Purpose

    AAV transfer plasmid encoding a circularized RNA barcode D under U6 promoter and a subcellular localization protein binder under hSyn promoter

    Depositor

    Xiao Wang

    Article

    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

    Inserts

    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    

    Use

    AAV

    Tags

    C-Far, Flag, and V5

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-T7-nSpRY(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES636)

    Plasmid

    #185491

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage nSpRY(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

    Depositor

    Raul Perez-Jimenez, Benjamin Kleinstiver

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage nSpRY(D10A)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-eGFP

    Expression

    Mammalian

    Mutation

    D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    CMV and T7

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRISPaint-mNeon-F-hgp100-PuroR [M1G]

    Plasmid

    #171801

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-FLAG-hgp100-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge