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pET-28b His6-TEV-SHP2-FL(R4A,R5A)

Plasmid

#245194

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 4:2025.07.0

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

R4A,R5A

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(R4A)

Plasmid

#245192

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 4:2025.07.0

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

R4A

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(E139A)

Plasmid

#245207

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 4:2025.07.0

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

E139A

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET30a-NanA-CSS-ET64

Plasmid

#234666

Purpose

Expression an ELP-tagged fusion enzyme of NanA and CSS

Depositor

Xianwei Liu

Article

Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

Inserts

sialic acid aldolase
CMP-sialic acid synthetase

Tags

ELP

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-RC2-KO-454TAG

Plasmid

#240063

Purpose

Express AAV2-KO (R585,588A) capsid with ncAA mutant at site 454

Depositor

Abhishek Chatterjee

Article

Erickson et al Bioconjug Chem. 2024 Jan 17;35(1):64-71.

Insert

AAV2-454-TAG, R585,588A

Use

AAV

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-RC2-454TAG

Plasmid

#240058

Purpose

Express AAV2 capsid with ncAA mutant at site 454

Depositor

Abhishek Chatterjee

Article

Erickson et al Bioconjug Chem. 2024 Jan 17;35(1):64-71.

Insert

AAV2 Rep Cap-454-TAG

Use

AAV

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pEt28a-H6-SUMO-SilkCatcher

Plasmid

#230902

Purpose

Bacterial expression of SilkCatcher

Depositor

Sesilja Aranko

Article

Fan et al Angew Chem Int Ed Engl. 2023 Mar 6;62(11

Insert

H6-SUMO-SilkCatcher

Tags

HisTag, thrombin, SUMO

Expression

Bacterial

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-His-SUMO-LS4

Plasmid

#228450

Purpose

LS4 protein expression vector for bacteria

Depositor

Keisuke Fukunaga

Article

Fukunaga et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

LS4

Tags

10xHis-SUMO

Expression

Bacterial

Promoter

T7

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET-His-SUMO-LS12

Plasmid

#228451

Purpose

LS12 protein expression vector for bacteria

Depositor

Keisuke Fukunaga

Article

Fukunaga et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

LS12

Tags

10xHis-SUMO

Expression

Bacterial

Promoter

T7

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pIVEX2.3-CS1M2b-3c-ON8-EGFP

Plasmid

#228452

Purpose

LS4-responsive riboswitch

Depositor

Keisuke Fukunaga

Article

Fukunaga et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

CS1M2b-3c-ON8 riboswitch-regulated EGFP

Use

Synthetic Biology

Promoter

T7

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pIVEX2.3-CS2-3c-ON8-EGFP

Plasmid

#228453

Purpose

LS12-responsive riboswitch

Depositor

Keisuke Fukunaga

Article

Fukunaga et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

CS2-3c-ON8 riboswitch-regulated EGFP

Use

Synthetic Biology

Promoter

T7

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-larE-larC

Plasmid

#232793

Purpose

Expression of L. plantarum larE and LarC genes in E. coli

Depositor

Robert Hausinger

Article

Nevarez et al mBio. 2024 Dec 16:e0340424. doi: 10.1128

Inserts

Pyridinium-3,5-bisthiocarboxylic acid mononucleotide synthase
Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein

Expression

Bacterial

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCBE-dGFP

Plasmid

#226861

Purpose

Constitutively expresses mCherry as a transfection marker. Expresses non-fluorescent EGFP, deactivated by Y93H mutation, that can be reactivated by active cytosine base editing

Depositor

Alexis Komor

Article

Osgood et al Methods Enzymol. 2025;712:351-404. doi:

Insert

EGFP

Expression

Mammalian

Mutation

Y93H

Promoter

CMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCBE-dGFP-gRNA

Plasmid

#226862

Purpose

Human gRNA expression vector targeting the Y93H mutation of non-fluorescent EGFP in plasmid pCBE-dGFP; for use with CBEs

Depositor

Alexis Komor

Article

Osgood et al Methods Enzymol. 2025;712:351-404. doi:

Insert

Sp-gRNA

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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dSpyCas9-E566-GS-LOV2-VPR

Plasmid

#231576

Purpose

Potent light-deactivated CRISPRa

Depositor

Dominik Niopek

Article

Wolf et al bioRxiv 2024.12.04.626757

Insert

dSpyCas9-VPR with an AsLOV2 insertion behind E566

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb12567

Plasmid

#219927

Purpose

PrCYC with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter CYC

Expression

Yeast

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb12566

Plasmid

#219926

Purpose

PrDGA with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter DGA

Expression

Yeast

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pVG329

Plasmid

#227771

Purpose

For genomic replacement of YOR1 gene in budding yeast

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

Genomic sequences flanking YOR1 in budding yeast (YOR1 Budding Yeast)

Expression

Yeast

Mutation

N/A

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pVG178

Plasmid

#227761

Purpose

Expresses a version of light-inducible transcription factor EL222 (Glu84Lys) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Glu84KLys mutant

Promoter

PGK1pr

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pVG144

Plasmid

#227760

Purpose

Expresses a version of light-inducible transcription factor EL222 (Val121Met) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Val121Met mutant

Promoter

PGK1pr

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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