We narrowed to 10,492 results for: nar

1. pRXG

   Plasmid

   #113643

   Purpose

   This construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.

   Depositor

   Jeffrey Barrick

   Article

   Monk et al ACS Synth Biol. 2016 Sep 20.

   Insert

   mRFP1-linker-sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-mROCK1-delta3

   Plasmid

   #101291

   Purpose

   Mammalian expression of Rock1-GFP for the study of localisation signaling.

   Depositor

   Alpha Yap

   Article

   Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

   Insert

   Rock1 (Rock1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   1-1080 aa

   Promoter

   CMV

   Available since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBTK1004 (pSL3)

   Plasmid

   #190999

   Purpose

   Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

   Depositor

   Jeffrey Barrick

   Article

   Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

   Insert

   red chromoprotein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   CP25

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pG3K-U6SC

   Plasmid

   #134754

   Purpose

   pGreen3 CRISPR/Cas9

   Depositor

   Qi-Jun Chen

   Article

   Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

   Insert

   CRISPR/Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   gRNA-U6, zCas9-35SC

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. E2F-1 wt-pGex2TK

   Plasmid

   #21668

   Depositor

   William Kaelin

   Article

   Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

   Insert

   E2F-1 (E2F1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   wild type

   Promoter

   Available since

   Sept. 4, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. CAGhP14

   Plasmid

   #84362

   Purpose

   express FLAG-HA tagged human PRDM14

   Depositor

   Danny Reinberg

   Article

   Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

   Insert

   PRDM14 (PRDM14 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   silent mutations at coding silent 1068CtoT, 1629T…

   Promoter

   CAG

   Available since

   Feb. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

7. pETCON-PTX-S1-220

   Plasmid

   #67747

   Purpose

   Pertussis Toxin Subunit 1 Yeast Display Plasmid

   Depositor

   Timothy Whitehead

   Article

   Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

   Insert

   PTxS1-220
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. TagRFP-T-Rabenosyn-5

   Plasmid

   #37537

   Depositor

   Silvia Corvera

   Article

   Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

   Insert

   Rabenosyn5 (RBSN Human)
   

   Use

   Tags

   TagRFP-T

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pG3B-U6SC

   Plasmid

   #134753

   Purpose

   pGreen3 CRISPR/Cas9

   Depositor

   Qi-Jun Chen

   Article

   Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

   Insert

   CRISPR/Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   gRNA-U6, zCas9-35SC

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMJ915v2+Nterm Spe1 +Cterm Age1 site

    Plasmid

    #88914

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. mOtop3_pcDNA3

    Plasmid

    #114679

    Purpose

    This construct was used to express mouse Otop3 in HEK-293 cells

    Depositor

    Emily Liman

    Article

    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

    Insert

    Otopetrin 3 (Otop3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET15b-mLin28A-cHIS

    Plasmid

    #137998

    Purpose

    Lin28-His6 was overexpressed in Escherichia coli Rosetta (DE3) cells

    Depositor

    Judy Lieberman

    Article

    O'Day et al J Biol Chem. 2015 Jul 17;290(29):17909-2

    Insert

    mouse Lin28A, CSD and ZnFs (residues 36–186)
    

    Use

    Tags

    C-terminal His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. CAG-mCherry-Otx2shRNA1

    Plasmid

    #73979

    Purpose

    Otx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of mCherry.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    shRNA that targets the mouse Otx2 gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. GST-RP2

    Plasmid

    #86072

    Purpose

    A bacterial expression plasmid encoding retinitis pigmentosa 2 (RP2) with N-terminus GST-tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    RP2 (RP2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. RP2-EGFP

    Plasmid

    #86073

    Purpose

    A mammalian expression plasmid encoding retinitis pigmentosa 2 (RP2) with C-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    RP2 (RP2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBTK1003 (pSL2)

    Plasmid

    #190998

    Purpose

    Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

    Depositor

    Jeffrey Barrick

    Article

    Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

    Insert

    red chromoprotein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CP25

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pG3B-U3Ub

    Plasmid

    #134750

    Purpose

    pGreen3 CRISPR/Cas9

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    CRISPR/Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    gRNA- OsU3, zCas9-Ubi1

    Available since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaPH

    Plasmid

    #22930

    Purpose

    Mammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PH domain

    Depositor

    Alexandra Newton

    Article

    Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

    Insert

    PHLPP1-deltaPH (PHLPP1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Includes 127-1205 aa. Deleted amino-terminal PH d…

    Promoter

    CMV

    Available since

    Feb. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

19. GL2 shRNA

    Plasmid

    #86083

    Purpose

    A lentiviral construct for building stable cell lines expressing GL2 shRNA via puromycin selection.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    control shRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-attBr-ZsGreen-attP

    Plasmid

    #51265

    Purpose

    Bxb1-responsive fluorescent reporter plasmid containing reverse complementary ZsGreen flanked by Bxb1 attB/P recognition sites in head-to-head orientation

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    attBr-ZsGreen-attP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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