We narrowed to 9,372 results for: BLI

1. psd44-G13GA

   Plasmid

   #46827

   Purpose

   expression of mutant G protein alpha 13 in mammalian cells

   Depositor

   Agnese Mariotti

   Insert

   GNA13 (GNA13 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   G225A

   Promoter

   PGK promoter

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

2. L57R

   Plasmid

   #34557

   Depositor

   Kevin Truong

   Insert

   L57 (CASP7 Human, A.Sativa (LOV2))
   

   Tags

   mRFP

   Expression

   Bacterial and Mammalian

   Mutation

   catalytic domain of caspase-7 was truncated at am…

   Promoter

   CMV, T7

   Available Since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pET22-6His-SUMO-Nsp10

   Plasmid

   #154960

   Purpose

   Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

   Depositor

   Justin Kollman

   Insert

   6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
   

   Tags

   6His-Thrombin-SUMO

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   July 21, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. K560_AviN_coBirA

   Plasmid

   #129766

   Purpose

   Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assays

   Depositor

   William Hancock

   Insert

   KHC (Khc Fly)
   

   Tags

   Avi-tag GLNDIFEAQKIEWH and His6

   Expression

   Bacterial

   Mutation

   Truncation at 559

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. BAZ2B

   Plasmid

   #39000

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   BAZ2B (BAZ2B Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   codon-optimized

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA_DiCre2_59.F2

   Plasmid

   #60209

   Purpose

   Expresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(19-59)

   Depositor

   Jean-Paul Herman

   Insert

   Cre recombinase (CD207 Human, Synthetic, Bacteriophage P1)
   

   Tags

   FKBP12(F36V) and Nuclear localisation signal

   Expression

   Mammalian

   Mutation

   FKBP12 has the F36V mutation; amino acids 19-59 o…

   Promoter

   CMV

   Available Since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. CAPZB2-TwinStrepII

   Plasmid

   #83194

   Purpose

   Expression of tagged protein in mammalian cells

   Depositor

   Peter Barr-Gillespie

   Insert

   CAPZB2 (Capzb Mouse)
   

   Tags

   Twin Strep Tag II

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti.puro.MCV.ER.RAZ.1

   Plasmid

   #114381

   Purpose

   This lentiviral plasmid expresses MCV tumor T antigens (truncated LT and small T) using the viral NCCR (non-coding control region). The sequence was amplifeied from MCV.R17a and truncated LT=330 aa.

   Depositor

   Sudhir Krishna, Reety Arora

   Insert

   MCV NCCR and tumor T antigen regions
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   MCV NCCR (viral own promoter)

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. GAPDHS

   Plasmid

   #39010

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   GAPDHS (GAPDHS Human)
   

   Tags

   TEV-His6-Flag

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1-Beta2-PAmcherry1

    Plasmid

    #114190

    Purpose

    studying clustering of Beta2 receptors by superresolution microscopy (PALM)

    Depositor

    Aleksandra Radenovic

    Insert

    Adrenergic receptor beta 2 (ADRB2 Human)
    

    Tags

    3HA, FLAG, and PAmcherry1

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pINDUCER20-FLAG-hSRF (#799)

    Plasmid

    #78345

    Purpose

    Doxycycline-inducible lentiviral overexpression of human SRF ORF with a FLAG tag

    Depositor

    Matthew Alexander, Louis Kunkel

    Insert

    SRF (SRF Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Promoter

    TRE2

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

12. 4L59

    Plasmid

    #60087

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    L3MBTL3:JMC057-A08:C223215 (L3MBTL3 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pMAL-c5X-BRT17

    Plasmid

    #67000

    Purpose

    Expresses malE-BRT17 in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    malE-BRT17
    

    Tags

    His6 and malE-Factor Xa

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV_CWSL.nEF.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3

    Plasmid

    #107736

    Purpose

    In the presence of Cre, can be used to express both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.

    Depositor

    Rylan Larsen

    Insert

    synaptophysin-GCaMP6s, mRuby3
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    nEF

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. ASH1L

    Plasmid

    #39051

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ASH1L (ASH1L Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. isoform PKC Delta (JCE594)

    Plasmid

    #182025

    Purpose

    yeast expression of mouse PKC Delta with yeast PKC1 promoter and ADH1 terminator

    Depositor

    Juan Carlos Igual

    Insert

    PKC Delta (Prkcd Mouse)
    

    Expression

    Mammalian

    Promoter

    yeast PKC1 promoter (-600 to -1)

    Available Since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pB6-Hipp11-td-sfGFP dre reporter-FNF-DTA

    Plasmid

    #139513

    Purpose

    B6J Hipp11-pCAG-rox-FNF-bGHpA-3xSV40pA-rox-td-sfGFP-WPRE-bpA allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    CAG rox stop rox td-sfGFP dre reporter
    

    Use

    Mouse Targeting; Dre/rox

    Expression

    Mammalian

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMBPcyto-TEV-BseRI

    Plasmid

    #157739

    Purpose

    empty vector adds TEV-protease-removable N-term His10 + maltose binding protein (without signal sequence); avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

    Depositor

    Debra Hansen

    Type

    Empty backbone

    Tags

    His10 + MBP (cytoplasmic expression; no signal se…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJK372

    Plasmid

    #73597

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-15 fatty acid desaturase (FAD3) with C-terminal c-myc epitope

    Depositor

    T. J. Kappock

    Inserts

    delta-12 fatty acid acetylenase
    delta-15 fatty acid desaturase
    

    Tags

    FLAG tag and c-myc tag

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. DIRAS2

    Plasmid

    #38819

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    DIRAS2 (diras2 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only