We narrowed to 7,184 results for: RAP

1. pBK1300-AAV-EFSNC-dCjCas9-MBD2(176-231)

   Plasmid

   #223145

   Purpose

   Expression of truncated MBD2 with dCjCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   MBD2 (MBD2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 176-231

   Promoter

   EF1a

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBK1299-AAV-EFSNC-dCjCas9-MBD1(529-592)

   Plasmid

   #223144

   Purpose

   Expression of truncated MBD1 with dCjCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   MBD1 (MBD1 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 529-592

   Promoter

   EF1a

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Tnnt2-Cre-U6-Lmna-sgRNA

   Plasmid

   #206178

   Purpose

   Expresses Cre recombinase specifically in cardiomyocytes and uses U6 promoter to express sgRNAs targeting murine Lmna exon 10

   Depositor

   Yuxuan Guo

   Article

   Sun et al JACC Basic Transl Sci. 2024 Aug 21;9(11)

   Insert

   Cre
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   cTnT

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBK1309-AAV-EFSNC-dSaCas9-MBD1(529-592)

   Plasmid

   #223154

   Purpose

   Expression of truncated MBD1 with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   MBD1 (MBD1 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 529-592

   Promoter

   EF1a

   Available since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBK1310-AAV-EFSNC-dSaCas9-MBD2(176-231)

   Plasmid

   #223155

   Purpose

   Expression of truncated MBD2 with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   MBD2 (MBD2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 176-231

   Promoter

   EF1a

   Available since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti HsATP13A2 P652_E653 del

   Plasmid

   #213700

   Purpose

   transfer plasmid for lentiviral vector production expressing Hs ATP13A2 P652_E653 del

   Depositor

   Veerle Baekelandt

   Article

   Colijn et al Neurogenetics. 2024 Jul 18. doi: 10.1007

   Insert

   human ATP13A2 P652_E653 del (ATP13A2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT, D962N

   Promoter

   CMV

   Available since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-cT3G-cERP2-HHEX

   Plasmid

   #222928

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of HHEX

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   HHEX (HHEX Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDV18 [punc-47::SNG-1::CRY2olig(535)]

   Plasmid

   #197598

   Purpose

   Expression of SNG-1::CRY2olig(535) in GABAergic motor neurons of C. elegans

   Depositor

   Alexander Gottschalk

   Article

   Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

   Insert

   Synaptogyrin-CRY2 (E490G + residues 1-535) (CRY2, sng-1 Mustard Weed, Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   E490G

   Promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDV12 [psng-1::SNG-1::CRY2olig(535)]

   Plasmid

   #197596

   Purpose

   Pan-neuronal expression of SNG-1::CRY2olig(535) in neurons of C. elegans

   Depositor

   Alexander Gottschalk

   Article

   Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

   Insert

   Synaptogyrin-CRY2 (E490G + residues 1-535) (CRY2, sng-1 Mustard Weed, Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   E490G

   Promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. LT3GEPIR-Rb1shRNA1

    Plasmid

    #220584

    Purpose

    To inducibly knockdown Rb1 expression

    Depositor

    Sean Lee

    Article

    Magrath et al Cancer Res. 2024 May 2;84(9):1426-1442.

    Insert

    Rb1 shRNA1 (RB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. LT3GEPIR-Rb1shRNA2

    Plasmid

    #220585

    Purpose

    To inducibly knockdown Rb1 expression

    Depositor

    Sean Lee

    Article

    Magrath et al Cancer Res. 2024 May 2;84(9):1426-1442.

    Insert

    Rb1 shRNA2 (RB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. LT3GEPIR-Rb1shRNA3

    Plasmid

    #220586

    Purpose

    To inducibly knockdown Rb1 expression

    Depositor

    Sean Lee

    Article

    Magrath et al Cancer Res. 2024 May 2;84(9):1426-1442.

    Insert

    Rb1 shRNA3 (RB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOTTC1789 - pAAV (flox-stop) mGrid1 390F gRNA A EF1a eGFP-KASH

    Plasmid

    #131684

    Purpose

    An adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a Cre-dependent guide RNA for mGrid1

    Depositor

    Christopher Richie

    Article

    Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

    Inserts

    EGFP-KASH
    SpCas9 sgRNA vs mouse GRID1
    

    Use

    AAV

    Tags

    KASH

    Expression

    Mutation

    Promoter

    EF1a and mU6-LSL (Cre dependent)

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AA324

    Plasmid

    #215953

    Purpose

    Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    rev{U6_v1; DR_v0[EnAs]; sgCD46_v4[EnAs]; DR_v1[EnAs]; sgCD47_v2[EnAs]; DR_v2[EnAs]; sgCD55_v4[EnAs];DR_v3[EnAs];sgCD81_v4[EnAs]}
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-PARP1-del.p119K120S–eGFP

    Plasmid

    #211554

    Purpose

    A piggyBac vector containing CMV-PARP1-del.p119K120S-eGFP-IRES-Neo cassette.

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Piggybac

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    deletion of amino acids 119K 120S

    Promoter

    CMV

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-N1-PARP1-delM43;F44I

    Plasmid

    #211579

    Purpose

    CMV driven PARP1-delM43;F44I -eGFP expressing construct with IRES-Neomycin selection

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Contains deletion of M43 and F44I

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pIG-749_HA-GD2-28z_CAR_TFAP4

    Plasmid

    #207490

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    TFAP4, HA-GD2-28z_CAR (TFAP4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIG-852_HA-GD2-28z_TFAP4-HA

    Plasmid

    #207502

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    TFAP4-HA, HA-GD2-28z_CAR (TFAP4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIG-858_CD19-BBz_CAR_TFAP4

    Plasmid

    #207503

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    TFAP4, CD19-BBz_CAR (TFAP4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5-FRT-TO-EGFR*-CUbo-GFP

    Plasmid

    #212815

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo-GFP in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Human, Budding Yeast)
    

    Use

    Tags

    8xHis and CUbo-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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