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pFBD-BirA_CDC20:161-P477

Plasmid

#210888

Purpose

Insect expression of human CDC20 fragment S161 to P477. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CDC20:S161-P477 (CDC20 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBD-BirA_CDC40:236-578

Plasmid

#210885

Purpose

Insect expression of human CDC40 fragment E236 to W578. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CDC40:E236-W578 (CDC40 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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JGI_018065

Plasmid

#158902

Purpose

Hydrocarbon Structures for Fuels and Chemicals

Depositor

Joint Genome Institute

Insert

WP_010983715_1

Use

Synthetic Biology

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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NK691

Plasmid

#120850

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Insert

Jumblelw1-mWasabi

Use

Choanoflagellate expression

Mutation

Jumblelw1 allele: T to C at nt 1109 causes L305P …

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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XP5 Xwnt-8

Plasmid

#16986

Depositor

Randall Moon

Insert

Xwnt-8 (wnt8a.L Frog)

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pPD30_69

Plasmid

#1444

Depositor

Andrew Fire

Type

Empty backbone

Tags

myo2-1090, myo25', IVSA, rf2128

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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L2531

Plasmid

#1607

Depositor

Andrew Fire

Type

Empty backbone

Tags

myo2-1090, myo25', IVSA, rf2128, IVSM, IVSL,…

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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mEmerald-NEEP21-C-18

Plasmid

#54201

Purpose

Localization: Endosomes, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

NEEP21

Tags

mEmerald

Expression

Mammalian

Mutation

D114G

Promoter

CMV

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mEmerald-NEEP21-N-18

Plasmid

#54202

Purpose

Localization: Endosomes, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

NEEP21

Tags

mEmerald

Expression

Mammalian

Mutation

D114G

Promoter

CMV

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
NK697

Plasmid

#120856

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Insert

pac-P2A-mTFP-Jumblelw1

Use

Choanoflagellate expression

Mutation

Jumblelw1 allele: T to C at nt 1109 causes L305P …

Available Since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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NK695

Plasmid

#120854

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Insert

pac-P2A-Jumblelw1-mTFP

Use

Choanoflagellate expression

Mutation

Jumblelw1 allele: T to C at nt 1109 causes L305P …

Available Since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPD20_97

Plasmid

#1437

Depositor

Andrew Fire

Type

Empty backbone

Tags

myo2-1090, myo25', IVSA, SV40NLS, lacZN, lac…

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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XE10 Xwnt-8 CS2+

Plasmid

#16865

Depositor

Randall Moon

Insert

Xwnt-8 (wnt8a.L Frog)

Use

Xenopus expression

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
LHP2260 - HA-INP53

Plasmid

#32198

Depositor

Linda Hicke

Insert

INP53 (INP53 Budding Yeast)

Tags

HA

Expression

Yeast

Promoter

Lac

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only
pGTVL2-SENP6

Plasmid

#196359

Purpose

protein expression of the GST-tagged catalytic domain

Depositor

Stefan Knapp

Insert

SENP6 catalytic domain

Tags

His6-GST-TEV

Expression

Bacterial

Mutation

contains only E652-A1095

Promoter

T7

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pPD80_08

Plasmid

#1456

Depositor

Andrew Fire

Type

Empty backbone

Tags

myo2-1090, myo25', IVSA, SV40NLS, gfpN, gfpS…

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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SV40 1: pBSSVD2005

Plasmid

#21826

Depositor

David Ron

Insert

SV40 Large T (SV40gp6 simian virus)

Expression

Mammalian

Mutation

likely expresses aa109-708 of the large T antigen

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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R777-E349 Hs.TIAM2

Plasmid

#70633

Purpose

Gateway ORF clone of human TIAM2 [NM_001010927.2] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

TIAM2 (TIAM2 Human)

Use

Gateway entry clone

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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L3790

Plasmid

#1596

Depositor

Andrew Fire

Type

Empty backbone

Tags

myo2-1090, myo25', IVSA, rf2128, T7p, gfpN, …

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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mEos2-NEEP21-N-18

Plasmid

#57407

Purpose

Localization: Endosomes, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

NEEP21

Tags

mEos2

Expression

Mammalian

Mutation

D114G

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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