Ready-to-use AAV9 particles produced from pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST (#109048). In addition to the viral particles, you will also receive purified pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST plasmid DNA. Cre-dependent expression of the anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites, translated from an internal ribosomal entry site (IRES) following mRuby3 expression. These AAV preparations are suitable purity for injection into animals.

CasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targeting

Catalytically inactive dCasRx with 2A-EGFP

mammalian expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)

Stable Dvl2 expression

AAV-CRISPR knockout plasmid with Cre recombinase driven by TBG promoter for direct in vivo genome editing in mouse liver

hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

pInducer20 with added blasticidin resistance

Tet-On construct to induce expression of tandem eGFP-mCherry tagged RAMP4 (ER marker)

Protein expression and purification of YWHAG_14-3-3

Cation channelrhodopsin CoChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Protein expression and purification of HSPA9_HSP70

Group II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”

Arabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coli

Expresses V5 and His-tagged human ANGPTL8 in mammalian cells

This plasmid expresses dsRed2 and puromycin resistance

hU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRs

Transient over-expression of human Axin1