We narrowed to 13,388 results for: 109

1. Plenti-CMV-MCS-RFP-SV-puro

   Plasmid

   #109377

   Purpose

   This plasmid expresses dsRed2 and puromycin resistance

   Depositor

   Behzad Gerami-Naini, Jonathan Garlick

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   dsRed2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDS-Red-N1 GABAA beta3

   Plasmid

   #109143

   Purpose

   The expression vector carries the red fluorescent protein tagged at the C-terminus of beta3 subunit of the GABAa receptor.

   Depositor

   Stefano Vicini

   Insert

   GABAa receptor
   

   Tags

   RFP

   Expression

   Mammalian

   Mutation

   beta3 subunit of the GABAa receptor

   Promoter

   CMV

   Available Since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXR001: EF1a-CasRx-2A-EGFP

   Plasmid

   #109049

   Purpose

   CasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targeting

   Depositor

   Patrick Hsu

   Article

   Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

   Insert

   CasRx
   

   Use

   CRISPR and Lentiviral

   Tags

   2A-eGFP, HA, and NLS

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pECFP18aaEYFP

   Plasmid

   #109330

   Purpose

   ECFP-EYFP FRET positive control

   Depositor

   Gabriele Kaminski Schierle

   Article

   Kaminski et al Methods Mol Biol. 2014;1076:445-54. doi:

   Insert

   ECFP-EYFP FRET positive control
   

   Expression

   Mammalian

   Mutation

   ECFP linked to EYFP with 18 aa linker GLRSRAQASNS…

   Promoter

   CMV

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEM705 Gsi (Ser)

   Plasmid

   #109352

   Purpose

   GsX chimera with Gαi C-terminus (Pertussis toxin insensitive mutant)

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Gsi chimera
   

   Tags

   None

   Expression

   Mammalian

   Mutation

   Changed Cys at position 391 to Serine (Pertussis …

   Promoter

   CAG

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEM705 Gsz

   Plasmid

   #109355

   Purpose

   GsX chimera with Gαz C-terminus

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Gsz chimera
   

   Tags

   None

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEM705 Gst (Ser)

   Plasmid

   #109354

   Purpose

   GsX chimera with Gαt C-terminus (Pertussis toxin insensitive mutant)

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Gst chimera
   

   Tags

   None

   Expression

   Mammalian

   Mutation

   Changed Cysteine at position 391 to Serine (Pertu…

   Promoter

   CAG

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEM705 Gso (Ser)

   Plasmid

   #109353

   Purpose

   GsX chimera with Gαo C-terminus (Pertussis toxin insensitive mutant)

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Gso chimera
   

   Tags

   None

   Expression

   Mammalian

   Mutation

   Changed Cysteine at position 391 to Serine (Pertu…

   Promoter

   CAG

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXR003: CasRx gRNA cloning backbone

   Plasmid

   #109053

   Purpose

   hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

   Depositor

   Patrick Hsu

   Article

   Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Flag-Axin1

    Plasmid

    #109370

    Purpose

    Transient over-expression of human Axin1

    Depositor

    Mariann Bienz

    Article

    Fiedler et al Proc Natl Acad Sci U S A. 2011 Feb 1;108

    Insert

    Axin1 (AXIN1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXR004: CasRx pre-gRNA cloning backbone

    Plasmid

    #109054

    Purpose

    hU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRs

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-C1 NFAT3

    Plasmid

    #10961

    Depositor

    Toren Finkel

    Article

    Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

    Insert

    NFAT3 (NFATC4 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTHSSe_48

    Plasmid

    #109246

    Purpose

    pSC101 var6 BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Expression

    Bacterial

    Promoter

    PJ23105

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas9-Geminin

    Plasmid

    #109401

    Purpose

    Expresses Cas9 fusion to Geminin degron domain

    Depositor

    Jean-Paul Concordet

    Article

    Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

    Insert

    Geminin (GMNN Human)
    

    Mutation

    Geminin degron fragment

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. actc1b-2PH-PLCd-mKate2

    Plasmid

    #109500

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to 2x the PH domain from Human phospholipase C delta 1 (PLCD1, codon optimised). Binds PtdIns(4,5)P2.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    actc1b-2PH-PLCd-mKate2
    

    Use

    Zebrafish plasmids

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28b-NmCas9-Flag

    Plasmid

    #109030

    Purpose

    Wild type Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

    Depositor

    Yan Zhang

    Article

    Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

    Insert

    CRISPR-associated endonuclease Cas9
    

    Tags

    Flag Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-TRAIL

    Plasmid

    #10953

    Depositor

    Bingliang Fang

    Article

    Kagawa et al Cancer Res. 2001 Apr 15. 61(8):3330-8.

    Insert

    Tumor necrosis factor alpha related apoptosis inducing ligand (TNFSF10 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV p16 INK4A

    Plasmid

    #10916

    Depositor

    Bob Weinberg

    Article

    Medema et al Proc Natl Acad Sci U S A. 1995 Jul 3. 92

    Insert

    p16 INK4 (CDKN2A Human)
    

    Expression

    Mammalian

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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19. NFAT luciferase reporter

    Plasmid

    #10959

    Depositor

    Toren Finkel

    Article

    Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

    Insert

    IL-2 promoter (IL2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXR002: EF1a-dCasRx-2A-EGFP

    Plasmid

    #109050

    Purpose

    Catalytically inactive dCasRx with 2A-EGFP

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Insert

    dCasRx
    

    Use

    CRISPR and Lentiviral

    Tags

    2A-eGFP, HA, and NLS

    Expression

    Mammalian

    Mutation

    R239A/H244A/R858A/H863A

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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