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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 109 results for: 109

Showing: 1 - 20 of 109 results

  1. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...human genome, the mouse genome is made up of 3 x 109 nucleotides (nt), and encodes 23,000 or so genes.... the gRNA will seek out its target among the 3 X 109 nt of genetic content in the mouse genome and the...

  3. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...discovery." Expert opinion on drug discovery 6.2 (2011): 109-127. PubMed PMID: 21532928. PubMed Central PMCID:...

  6. CRISPR References and Information

    Type
    Collection
    ...Bacteria: pCRISPR new spacer cloning pCRISPR PDF, 109 KB Mendenhall and Myers Mammalian: FLAG tagging endogenous...injection protocol pU6-BbsI-chiRNA ; phsp70-Cas9 PDF, 109 KB Orkin and Bauer Protocol for Genomic Deletions...

  8. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...fundamentals and recent instrumentation. Cytotechnology 64:109–130 . https://doi.org/10.1007/s10616-011-9415-0 Additional...

  10. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...and 8 in the rat nigrostriatal system. J Neurochem 109, 838–845. PubMed PMID: 19250335. PubMed Central PMCID...

  11. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...combinatorial cell-subclass-specific labeling. Neuron , 109 (9), 1449–1449. https://doi.org/10.1016/j.neuron.2021.03.011...208157 pAAV-AiE0410m-minBglobin-SYFP2-WPRE3-BGHpA AiP12109 AiE0410m SYFP2 Oligodendrocytes Whole Brain 230388...

  12. Validated gRNA Sequences

    Type
    Collection
    ...TTTCCAGGATTACGTAATAG 60967 cut S. pyogenes 24967838 Mashimo unc-109(n499) C. elegans GGAACTCGTGTCAAAACAAC 59932 cut S...

  14. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...comparison, the DNA replication error rate is 1 per 109 bp. Unlike wt Cas9, CRISPR-X produces few indels,...

  15. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...diseases . Cytokine & Growth Factor Reviews, 80 , 109–120. https://doi.org/10.1016/j.cytogfr.2024.09.003...Research, 25 (3), 489–499. https://doi.org/10.1007/s11095-007-9431-0 (Link opens in a new window) PMID: 17763830...Virology, 85 (Pt 8), 2209–2214. https://doi.org/10.1099/vir.0.79940-0 (Link opens in a new window) PMID...

  16. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...Gen Virol., 73 (Pt 3), 653–660. https://doi.org/10.1099/0022-1317-73-3-653. PMID 1372038. Randall, R. E...Virol., 68 (Pt 11), 2769–2780. https://doi.org/10.1099/0022-1317-68-11-2769. PMID 2445904.   New tool...elegans. Genetics, 228(2), iyae126. https://doi.org/10.1093/genetics/iyae126.   Gene disruption in Mycobacterium...

  17. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...EMBO Journal, 17(17), 5201–5213. https://doi.org/10.1093/emboj/17.17.5201 del Solar, G., Giraldo, R., Ruiz-Echevarría...Acids Research, 46(12), 6140–6151. https://doi.org/10.1093/nar/gky457 Park, K., Han, E., Paulsson, J., & Chattoraj...EMBO Journal, 20(24), 7323–7332. https://doi.org/10.1093/emboj/20.24.7323 Resources at Addgene Read our...

  18. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...Human Molecular Genetics 10:2109–2121 . https://doi.org/10.1093/hmg/10.19.2109 Sandrin V, Boson B, Salmon...

  19. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ... Acids Research 48:2841–2852 . https://doi.org/10.1093/nar/gkaa124 Martin AS, Salamango DJ, Serebrenik...Nucleic Acids Research 46:e84–e84 . https://doi.org/10.1093/nar/gky332 Standage-Beier K, Tekel SJ, Brookhouser... Acids Research 47:e120–e120 . https://doi.org/10.1093/nar/gkz713 Additional resources on the Addgene ...

  20. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...Nucleic Acids Research 28:292–292 . https://doi.org/10.1093/nar/28.1.292 Sauna ZE, Kimchi-Sarfaty C (2011) ... Nucl Acids Res 15:1281–1295 . https://doi.org/10.1093/nar/15.3.1281 Slimko EM, Lester HA (2003) Codon... base pair. EMBO Rep 1:18–23 . https://doi.org/10.1093/embo-reports/kvd001 Additional resources on the...
Showing: 1 - 20 of 109 results