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Plasmid#235785Purpose5' Homology psbA in the M1 position used for genome integration at the psbA locusDepositorInsert5' Homology region of the psbA locus of Chlamydomonas reinhardtii chloroplast CC-125
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_1_007_petB_5'Hom_cr
Plasmid#235787Purpose5' Homology petB in the M1 position used for genome integration at the petB locusDepositorInsert5' Homology region of the petB locus of Chlamydomonas reinhardtii chloroplast CC-125
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_G_0_1_005_5C1SF
Plasmid#235789Purpose5' Connector 1 Short Forward in the M1 position used to dictate position for lvl2 assemblyDepositorInsert5' Connector 1 Short Forward
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR-B/c
Plasmid#227962PurposeEntry plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.DepositorInsertB/c
UseTagsExpressionMutationPromoterNoneAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCIFR-3
Plasmid#233295PurposeIntegration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.DepositorInsertmsfGFP
UseTagsExpressionMutationPromoterp14GAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT81: pTarget(ZooTsy)
Plasmid#200876PurposeTarget plasmid containing attachement site for ZooTsy.DepositorInsertZooTsy_comM
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT112: pTarget(AjTn6022)_U50D50
Plasmid#200862PurposeTarget plasmid containing attachement site for AjTn6022.DepositorInsertAjTn6022_comM
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG006
Plasmid#231310PurposeVector part for BEVA; BEVA2.0 Position 3 p15A narrow host range origin of replication with oriT, CmR.DepositorInsertBEVA2.0 Position 3 p15A narrow host range origin of replication with oriT, CmR.
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG007
Plasmid#231311PurposeVector part for BEVA; BEVA2.0 Position 3 pMB1 narrow broad host range origin of replication with oriT, CmR.DepositorInsertBEVA2.0 Position 3 pMB1 narrow broad host range origin of replication with oriT, CmR.
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pB-DEST (JDW 1205)
Plasmid#229821PurposeA PiggyBac destination vector compatible with gateway cloning system.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
empty_pK7fWG2_IVD_FP611
Plasmid#228371PurposeEmpty dual expression vector for determining subcellular localization for protein of interestDepositorTypeEmpty backboneUseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNDGG001
Plasmid#231307PurposeVector part for BEVA; BEVA2.0 Position 2 Km/Nm antibiotic resistance via nptII, CmR/KmR/NmR.DepositorInsertBEVA2.0 Position 2 Km/Nm antibiotic resistance via nptII, CmR/KmR/NmR.
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-1:ChrA-C63S-C66S
Plasmid#225082PurposeExpresses precursor peptide ChrA-C63S/C66S in E.coliDepositorInsertChrA-C63S/C66S
UseTagsHis6ExpressionBacterialMutationC63S/C66SPromoterT7Available sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLei-sfGFP-D134*
Plasmid#191110PurposeAn E. coli sfGFP reporter with one TAG at position 134DepositorInsertsuperfolder green fluorescence protein
UseTagsHis tagExpressionBacterialMutationchanging D134 to TAGPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLei-sfGFP-V2*D134*
Plasmid#191111PurposeAn E. coli sfGFP reporter with two TAG at positions 2 & 134DepositorInsertsuperfolder green fluorescence protein
UseTagsHis tagExpressionBacterialMutationchanging V2 & D134 to TAGPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-AtTIR1-Myc-MBP-HA
Plasmid#222709PurposeExpression AtTIR1-Myc and MBP-HA in in bacterial cellsDepositorInsertAtTIR1 (TIR1 Mustard Weed)
UseTagsmycExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
dCas9 fused to scFv (anti-spike)
Plasmid#186421PurposeExpression of dCas9 with C-terminal nanobody fusion recognizing spike protein from SARS-CoV-2DepositorInsertdCas9-scFv fusion (anti-SARS-CoV-2 spike)
UseCRISPRTags6HisExpressionMutationPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-yhaM-RFP
Plasmid#207651PurposeOverexpression of native L-cysteine desulfidase and RFP in E. coli MG1655DepositorInsertyhaM
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPC53
Plasmid#209962PurposeContains Level 0 Part: 5' Connector (5C1CSN with double terminator Tcat+sai) for the construction of Level 1 plasmidsDepositorInsert5' Connector (5C1CSN_Tcat+sai)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr
Plasmid#218767Purposeencodes EcTyrRS-pAAFRS-9 and EcTyr-tRNA-TAGDepositorInsertEcTyrRS-pAAFRS-9
UseTagsExpressionBacterialMutationY37G, L71V, D182C, F183Y, L186C, D265RPromotertacIAvailable sinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only