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pMCL-HA-MAPKK2-KW71[delta(48-55)/S222D/S226D]

Plasmid

#40821

Depositor

Natalie Ahn

Article

Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

Insert

MAP2K2 (MAP2K2 Human)

Tags

HA

Expression

Mammalian

Mutation

delta(48-55) + S222D + S226D

Promoter

CMV

Available Since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only
JH401: pMVP/Ad/RGD-PK-DEST

Plasmid

#121846

Purpose

pMVP destination vector, empty RGD/PK fiber-modified adenovirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Adenoviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pHsp70B (GB0155)

Plasmid

#68172

Purpose

Provides the Arabidopsis thaliana heat shock protein 70B promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtHsp70B promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pMC_mNG2(11)_BSDminus_F0

Plasmid

#184162

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mNG2(11)
bsd

Use

CRISPR

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pWL502-crRNA

Plasmid

#174383

Purpose

The plasmids carrying a mini-CRISPR array were used to express crRNAs; article demonstrates use in Haloferax mediterranei

Depositor

Jing Han

Article

Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

Insert

a crRNA targeting the template strand

Use

CRISPR; Archaeal expression

Expression

Bacterial

Promoter

PhaR promoter

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab5aa

Plasmid

#109648

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab5aa

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab5aa (rab5aa Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab5ab

Plasmid

#109649

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab5ab

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab5ab (rab5ab Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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HB901: pMVP (L3-L2) polyA

Plasmid

#121746

Purpose

pMVP L3-L2 entry plasmid, contains human growth hormone polyadenylation signal (polyA) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Polyadenylation signal (polyA)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IF701: pMVP (L5-L4) APEX2

Plasmid

#121725

Purpose

pMVP L5-L4 entry plasmid, contains APEX2 for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term APEX2 to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

APEX2 (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LC501: pMVP (L3-L2) mCherry + polyA

Plasmid

#121765

Purpose

pMVP L3-L2 entry plasmid, contains mCherry + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term mCherry fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

mCherry + polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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