We narrowed to 50,311 results for: ANG

1. pHAGE-IRES-puro-NLS-dPguCas13b-EGFP-NLS-3xFlag

   Plasmid

   #132400

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPguCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   H151A; H1121A

   Promoter

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. U6-5'+3' MS2-CircRNA

   Plasmid

   #213752

   Purpose

   For circular RNA-mediated prime editor using U6-5'+3' MS2-CircRNA in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   5' ribozyme, 5' ligation sequences, 5' MS2, 3' MS2, 3' ligation sequences, 3' ribozyme
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. MCP-M-MLV RT∆RNase H

   Plasmid

   #213749

   Purpose

   For circular RNA-mediated prime editor using MCP-M-MLV RT∆RNase H in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   MCP-M-MLV RT∆RNase H
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC0043-PspCas13b crRNA backbone

   Plasmid

   #103854

   Purpose

   For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6-BbsI-BbsI-PspCas13b DR-polyT
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. Fragment1 with TLS2

   Plasmid

   #196983

   Purpose

   Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Intermediate fragment for assembly of gRNA-TLS2 construct
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-SpRY-ABE8e

   Plasmid

   #185671

   Purpose

   Expresses SpRY-ABE8e in mammalian cells

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   ecTadA(8e)-nSpRY
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   SpRY D10A

   Promoter

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Perturb-FISH

   Plasmid

   #220626

   Purpose

   expression of gRNAs under U6T7 promoter

   Depositor

   Samouil Farhi

   Article

   Binan et al Cell. 2025 Mar 10:S0092-8674(25)00197-7.

   Insert

   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   BsmBI

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221_EGFP

   Plasmid

   #25899

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   EGFP
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19/mChe-PM

   Plasmid

   #183168

   Purpose

   Construct for transient expression of plasma membrane-targeted mCherry in plants.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   mCherry-PM
   

   Use

   Tags

   OsRac3, O. sativa rac-like GTP-binding protein 3

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. N8his-GFPenhancer-GGGGS4-LaG16

    Plasmid

    #140442

    Purpose

    We designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.

    Depositor

    Motoyuki Hattori

    Article

    Zhang et al Sci Rep. 2020 Apr 10;10(1):6239. doi: 10

    Insert

    GFPenhancer-GGGGS4-LaG16
    

    Use

    Tags

    8 histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-IRES-puro-NLS-dPspCas13b-EGFP-NLS-3xFlag

    Plasmid

    #132397

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPspCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H133A; H1058A

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-EGFP-IRES-puro

    Plasmid

    #128652

    Purpose

    mammalian expression of EGFP with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    Wild type

    Promoter

    CMV

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDualIN-SMN2

    Plasmid

    #214430

    Purpose

    Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

    Insert

    SMN2 EXON 7 (SMN2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEVOL-pylT-N346A/C348A

    Plasmid

    #127411

    Purpose

    Double mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethers

    Depositor

    Wenshe Liu

    Article

    Wang et al J Am Chem Soc. 2012 Feb 15;134(6):2950-3

    Insert

    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N346A-C348A

    Promoter

    araBAD

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAV-CAG-Sun1-GFP-WPRE-pA

    Plasmid

    #220752

    Purpose

    Expression of Sun1GFP for nuclei isolation

    Depositor

    Vijay Samineni

    Article

    Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

    Insert

    Sun1-GFP-WPRE
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRCT

    Plasmid

    #60621

    Purpose

    Plasmid encoding iCas9, tracrRNA and crRNAs

    Depositor

    Huimin Zhao

    Article

    Bao et al ACS Synth Biol. 2014 Sep 19.

    Inserts

    iCas9
    tracrRNA
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Yeast

    Mutation

    changed Aspartate 147 to Tyrosine, Proline 411 to…

    Promoter

    RPR1p and TEF1p

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-EKAR4

    Plasmid

    #174437

    Purpose

    Improved cyan/yellow FRET-based ERK kinase activity reporter.

    Depositor

    Jin Zhang

    Article

    Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

    Insert

    EKAR4
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUCmini-iCAP-PHP.AX

    Plasmid

    #195218

    Purpose

    AAV capsid for broadly targeting cell types in the CNS (neurons including both excitatory and inhibitory neurons, astrocytes)

    Depositor

    Viviana Gradinaru

    Article

    Jang et al Nat Biotechnol. 2023 Jan 26. doi: 10.103

    Insert

    Synthetic construct isolate AAV-PHP.AX VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. IBMc396

    Plasmid

    #161954

    Purpose

    Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHH

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Flag-cIAP2/pRK5

    Plasmid

    #27973

    Depositor

    Xiaolu Yang

    Article

    Hu et al J Clin Invest. 2006 Jan . 116(1):174-81.

    Insert

    CIAP2 (BIRC3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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