We narrowed to 19,454 results for: gateway
-
Plasmid#176026PurposeMultisite gateway vector for expression of nCas9n with blue reporter. Parton lab clone ENTDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnCas9n-P2A-TagBFP2
UseGateway CloningAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-Lifeact-mEos2-NS
Plasmid#115549PurposeMultisite gateway vector for 5' tagging with Lifeact-mEos2. Used to make 115563. Parton lab clone IDADepositorInsertLifeact-mEos2
Available sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 A66V
Plasmid#166817PurposeGateway pDONR vector encoding NLRP1 with the patient mutation A66V (Zhong et al., 2016) (no stop codon)DepositorAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA2
Plasmid#145370PurposeGateway destination vector for cell-free and mamalian expression with N-term 8xHis-tag and mCherry tagDepositorTypeEmpty backboneTagsHis and mCherryExpressionBacterialAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292-D570A
Plasmid#129679PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAaCas12b
UseCRISPR; Gateway compatible aacas12b entry cloneExpressionPlantMutationD570A; AaCas12b is rice codon optimizedAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-actc1b-ccdb-mKate2
Plasmid#109731PurposeMultisite gateway destination vector for muscle specific expression of constructs tagged at the 3' end with mKate2DepositorTypeEmpty backboneUseZebrafish plasmidsAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-actc1b-mKate2-ccdb
Plasmid#109732PurposeMultisite gateway destination vector for muscle specific expression of constructs tagged at the 5' end with mKate2DepositorTypeEmpty backboneUseZebrafish plasmidsAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-ATG18
Plasmid#109556PurposeMultisite gateway vector for 3' tagging with yeast ATG18 (PLEKHA3). Binds PtdIns(3,5P)2DepositorInsertyeast ATG18 (PLEKHA3)
UseZebrafish plasmidsAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5E-6xgata2aECE
Plasmid#132969Purposegata2a endothelial enhancer (x6) in p5E Gateway vectorDepositorInsertgata2a endothelial enhancer (x6)
UseGateway vectorAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
LC501: pMVP (L3-L2) mCherry + polyA
Plasmid#121765PurposepMVP L3-L2 entry plasmid, contains mCherry + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term mCherry fusion to gene of interest.DepositorInsertmCherry + polyA
UseGateway Cloning and Synthetic BiologyAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IF701: pMVP (L5-L4) APEX2
Plasmid#121725PurposepMVP L5-L4 entry plasmid, contains APEX2 for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term APEX2 to gene of interest.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAPEX2 (open)
UseGateway Cloning and Synthetic BiologyAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
Moe-pDONR221
Plasmid#124239PurposeGateway entry clone of Drosophila Moesin cDNA encoding the D isoform.DepositorInsertDrosophila Moesin (Moe Fly)
ExpressionInsectAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ140
Plasmid#99895PurposeEmpty vector with attL5-attL2 recombination sites for Gateway CloningDepositorTypeEmpty backboneUseCRISPR and TALENAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3XFLAG-wtGATA6-3XAU1_RNAi resistant
Plasmid#72922PurposeGateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human wtGATA6DepositorInsertGATA Binding Protein 6 (GATA6 Human)
UseGateway CloningTags3XAU1 and 3XFLAGExpressionBacterialPromoterTRE tightAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT S6K (E389-deltaCT)
Plasmid#58507PurposeGateway entry vector for an inducible HA-tagged rat S6K1-E389-deltaCTDepositorInsertRps6kb1 (Rps6kb1 Rat)
UseGateway CloningTagsHAMutationT389E, stop codon at amino acid 402Available sinceAug. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT Tgfbr3-HA
Plasmid#58497PurposeGateway entry vector for an inducible HA-tagged mouse Tgfbr3DepositorAvailable sinceAug. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEG R1-ChlorR-R4 #BGV049
Plasmid#48943PurposeContains the Gateway selection cassette (ccdB ChlorR) flanked by AttR1 and AttR4 sites for the creation of Destination vectorsDepositorTypeEmpty backboneUseGateway CloningPromoternaAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_HyPer7RgDAAO
Plasmid#217746PurposeDonor vector for gateway cloning of HyPer7 DAAO, a fusion protein used to measure the uptake of D-amino acids across the plasma membrane.DepositorInsertHyPer7 D amino acid oxidase
TagsNuclear export signalExpressionMammalianMutationFused DAAO to the C-terminus of HyPer7 using a Gl…Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-ING2
Plasmid#109554PurposeMultisite gateway vector for 3' tagging with 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5PDepositorInsert3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised
UseZebrafish plasmidsAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW/TOPO-ELK3
Plasmid#98622PurposeGateway entry TOPO TA cloning vector for ELK3DepositorAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only