We narrowed to 10,132 results for: yeast

1. p426 3-F GW

   Plasmid

   #58556

   Purpose

   pRS426 carrying Gateway cassette for three inserts

   Depositor

   Leonid Kruglyak

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Nov. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS415-SSM1

   Plasmid

   #11003

   Depositor

   Francisco Malagon

   Insert

   SSM1 (SDT1 Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   Wild type

   Available Since

   Dec. 29, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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3. YEplac181-SSM1

   Plasmid

   #11002

   Depositor

   Francisco Malagon

   Insert

   SSM1 (SDT1 Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   Wild type

   Available Since

   Dec. 16, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. BB3cK_pGAP_23*_pTEF_Cas9

   Plasmid

   #104909

   Purpose

   hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   June 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFBP-2_6.sensor

   Plasmid

   #162800

   Purpose

   Yeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #2_6

   Depositor

   Matthias Heinemann

   Article

   Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

   Insert

   2_6_RNA-device
   

   Expression

   Yeast

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. BB3cN_pGAP_23*_pPFK300_Cas9

   Plasmid

   #104911

   Purpose

   hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. BB3cH_pGAP_23*_pLAT1_Cas9

   Plasmid

   #104907

   Purpose

   hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-C1-hApg5

   Plasmid

   #22952

   Depositor

   Noboru Mizushima

   Article

   Mizushima et al J Biol Chem. 1998 Dec 18. 273(51):33889-

   Insert

   Homo sapiens ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   July 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGTQL1211YN

   Plasmid

   #61704

   Purpose

   Gateway compatible BiFC vector (pEarleyGate201-YN)

   Depositor

   Yuhai Cui

   Article

   Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

   Type

   Empty backbone

   Use

   Bifc assay

   Tags

   YFP-N and HA

   Expression

   Plant

   Promoter

   35S

   Available Since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGTQL1221YC

    Plasmid

    #61705

    Purpose

    Gateway compatible BiFC vector (pEarleyGate202-YC)

    Depositor

    Yuhai Cui

    Article

    Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

    Type

    Empty backbone

    Use

    Bifc assay

    Tags

    YFP-C and FLAG

    Expression

    Plant

    Promoter

    35S

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pV1524

    Plasmid

    #111431

    Purpose

    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pS1643 (pFA6-NATMX-CDC42pr-yGFP1-10)

    Plasmid

    #86420

    Purpose

    N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    CDC42pr-GFP1-10
    

    Tags

    GFP1-10

    Expression

    Yeast

    Available Since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK-caCas9-NatMX-Neut5L

    Plasmid

    #89576

    Purpose

    caCas9 integrating vector into the Neut5L locus

    Depositor

    George Church

    Article

    Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYES260-sc.eEF2

    Plasmid

    #85119

    Purpose

    yeast expression of EEF2

    Depositor

    Pal Falnes

    Article

    Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

    Insert

    EFT1/EFT2 (EFT2, EFT1 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-FLAG-Rheb-N153T

    Plasmid

    #19997

    Depositor

    Fuyuhiko Tamanoi

    Article

    Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

    Insert

    Ras homolog enriched in brain 1 - N153T (RHEB Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed Asparagine 153 to Threonine

    Available Since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pYES260-sc.eEF2 K509A

    Plasmid

    #85120

    Purpose

    yeast expression of mutant EEF2

    Depositor

    Pal Falnes

    Article

    Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

    Insert

    EFT1/EFT2 (EFT2, EFT1 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    K509A

    Promoter

    GAL1

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pV1393

    Plasmid

    #111430

    Purpose

    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pfa6a PhoCl-TsCC(B)::KANMX

    Plasmid

    #185906

    Purpose

    Plasmid for cloning PhoCl fused to TsCC(B) into yeast.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    PhoCl-TsCC(B)
    

    Tags

    PhoCl fluorophore with C-terminal TsCC(B)

    Expression

    Yeast

    Promoter

    SP6

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. p415 TEF roGFP2‐Tsa2ΔCR

    Plasmid

    #83238

    Purpose

    The genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

    Depositor

    Tobias Dick

    Article

    Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

    Insert

    roGFP2-Tsa2dCr
    

    Tags

    roGFP2 fusion with Tsa2dCr

    Expression

    Yeast

    Promoter

    TEF

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET26b_Nb.b201

    Plasmid

    #131404

    Purpose

    E. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albumin

    Depositor

    Andrew Kruse

    Article

    McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

    Insert

    Nanobody b201
    

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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