We narrowed to 6,289 results for: ATS

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pCE059-SiT-ddCas12a(E993A)-3xNLS-[Activ]-triplex-DR-M13-DR-pA

Plasmid

#202043

Purpose

Encodes a DNase-dead AsCpf1 fused to a 3x SV40 nuclear localization signal (NLS) and [Activ] transactivation domain. 3' UTR has a MALAT1 triplex, dCas12a direct repeats (DR), and a crRNA cloning site.

Depositor

Darren Cusanovich

Article

Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1

Insert

ddCas12a(E993A)-3xNLS-[Activ]

Expression

Mammalian

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCE059-SiT-ddCas12a(E993A)-3xNLS-VP64-triplex-DR-M13-DR-pA

Plasmid

#202044

Purpose

Encodes a DNase-dead AsCpf1 fused to a 3x SV40 nuclear localization signal (NLS) and VP64 transactivation domain. 3' UTR has a MALAT1 triplex, dCas12a direct repeats (DR), and a crRNA cloning site.

Depositor

Darren Cusanovich

Article

Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1

Insert

ddCas12a(E993A)-3xNLS-VP64

Expression

Mammalian

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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MZB127

Plasmid

#217836

Purpose

Bacterial expression of human Arf1 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

Arf1, rtxA (ARF1 Vibrio cholerae, Human)

Tags

10x His and CPD

Expression

Bacterial

Mutation

Q71L

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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poly H35S SUMO1

Plasmid

#221071

Purpose

Expression of poly H35S SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of H35S SUMO1 each separated by (GGS)4 (SUMO1 Human)

Tags

His and MBP

Expression

Bacterial

Mutation

H35S

Promoter

tac

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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poly E67R SUMO1

Plasmid

#221072

Purpose

Expression of poly E67R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of E67R SUMO1 each separated by (GGS)4 (SUMO1 Human)

Tags

His and MBP

Expression

Bacterial

Mutation

E67R

Promoter

tac

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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poly H35K SUMO1

Plasmid

#221070

Purpose

Expression of poly H35K SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of H35K SUMO1 each separated by (GGS)4 (SUMO1 Human)

Tags

His and MBP

Expression

Bacterial

Mutation

H35K

Promoter

tac

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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poly H35R SUMO1

Plasmid

#221069

Purpose

Expression of poly H35R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of H35R SUMO1 each separated by (GGS)4 (SUMO1 Human)

Tags

His and MBP

Expression

Bacterial

Mutation

H35R

Promoter

tac

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pGG-PIP06

Plasmid

#196744

Purpose

Contains BAK1 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pWT-IFl_WT-IFr

Plasmid

#192207

Purpose

Wildtype I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Wildtype I-F Leader and wildtype I-F Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pScr-IFl_WT-IFr

Plasmid

#192208

Purpose

Scrambled I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Scrambled I-F Leader and wildtype I-F Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-IFr

Plasmid

#192209

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus I-F Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-IEr

Plasmid

#192210

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus I-E Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-ICr

Plasmid

#192211

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus I-C Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-IIAr

Plasmid

#192212

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus II-A Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IEl_Con-IEr

Plasmid

#192213

Purpose

Consensus I-E CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-E Leader and Consensus I-E Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-ICl_Con-ICr

Plasmid

#192214

Purpose

Consensus I-C CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-C Leader and Consensus I-C Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IIAl_Con-IIAr

Plasmid

#192215

Purpose

Consensus II-A CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus II-A Leader and Consensus II-A Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pX1-IFl_Con-IFr

Plasmid

#192216

Purpose

Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

I-F leader and I-F repeat construct not studied further in this paper (X1)

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pX2-IFl_Con-IFr

Plasmid

#192217

Purpose

Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

I-F leader and I-F repeat construct not studied further in this paper (X2)

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-455-WT

Plasmid

#215881

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-455-WT (MIR455 Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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