We narrowed to 10,454 results for: ESP

1. pET28a-RBP-GFP(S45C)-ZdK1

   Plasmid

   #185791

   Purpose

   Encodes green flourescent protein linked to RNA binding peptide (on N terminal end ) and Z domain fragment (variant 1) of Staphylococcal protein A (on C-terminal) via GS-linker

   Depositor

   Lizhong He

   Article

   Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

   Insert

   RBP-sfGFP(S45C)-ZdK1
   

   Tags

   10xHis, TEV protease cleavage site after His Tag

   Expression

   Bacterial

   Mutation

   Ser to Cys mutation at start of GFP, residue 45 i…

   Promoter

   T7

   Available Since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. LRRK2-K1906M

   Plasmid

   #35946

   Depositor

   Zhaoyang Feng

   Article

   Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

   Insert

   LRRK2 (LRRK2 Human)
   

   Tags

   His and Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

3. SHIP2 WT pBABE-His-Xpress

   Plasmid

   #126584

   Purpose

   Retroviral infection of MEF cells (puromycin selection)

   Depositor

   Christophe Erneux

   Article

   Zhang et al Cell Signal. 2007 Oct;19(10):2194-200. d

   Insert

   SHIP2 (INPPL1 Human)
   

   Use

   Retroviral

   Tags

   His Xpress (N-terminal)

   Available Since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500)

   Plasmid

   #115142

   Purpose

   CAG promoter expression plasmid for human codon optimized Mb3Cas12a nuclease with C-terminal NLS and HA tag

   Depositor

   Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized Mb3Cas12a
   

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL2 p15 (-756/+68) SBR2 Mut 3' box

   Plasmid

   #15711

   Depositor

   Joan Massague

   Article

   Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

   Insert

   p15 promoter (CDKN2B Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Mut 3' box in SBR2. The 3' box contains…

   Available Since

   Sept. 7, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pT2ADW-lox-mCherryNLS-pA-lox-4493NES

   Plasmid

   #138374

   Purpose

   Booster-PKA for the generation of mice

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

   Insert

   lox-mCherry-lox-Booster-PKA
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNAIIIB rac1 QL

   Plasmid

   #61633

   Purpose

   mammalian expression of rac1 constitutively active mutant

   Depositor

   JS Gutkind

   Article

   Coso et al Cell. 1995 Jun 30;81(7):1137-46.

   Insert

   Rac1 (RAC1 Human)
   

   Expression

   Mammalian

   Mutation

   Q61L, constitutively active

   Promoter

   CMV

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pCMV-Tag2-Sox9 T236A/T240A

   Plasmid

   #111458

   Purpose

   Transient expression of Flag-tagged human Sox9 with T236A/T240A mutation

   Depositor

   Sharon Pine

   Article

   Hong et al Nucleic Acids Res. 2016 Oct 14;44(18):88

   Insert

   Sox9 (SOX9 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T236A and T240A

   Promoter

   CMV

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5FRT/TO-PLAA-Strep-HA

   Plasmid

   #113484

   Purpose

   Expression of human PLAA with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   PLAA (PLAA Human)
   

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFRT_TO_FlagHA_CELF1

    Plasmid

    #106099

    Purpose

    Inducible gene expression of FlagHA - CELF1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    CELF1 (CELF1 Human)
    

    Tags

    Flag and HA

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-tight-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid

    #102890

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST27-GST-ATXN1 FL(30Q)

    Plasmid

    #21753

    Depositor

    Huda Zoghbi

    Article

    Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 32 amino acids (30 Q…

    Promoter

    CMV

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

13. PB-tight-DDdCas9VPPH-T2A-GFP-IRES-Neo

    Plasmid

    #102892

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCin4-3xFLAG-MKL1 S454A

    Plasmid

    #19844

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 S454A (MRTFA Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Serine 454 to Alanine

    Available Since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

15. gRNA-hIRF-1 #12/pSIR

    Plasmid

    #61080

    Purpose

    Expresses a guide RNA (gRNA) to target human IRF-1 promoter

    Depositor

    Hodaka Fujii

    Article

    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

    Insert

    gRNA_hIRF1 promoter #12 (IRF1 Human)
    

    Use

    CRISPR and Retroviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-RNF8-N-terminus(1-111)

    Plasmid

    #64671

    Purpose

    Expresses only N terminus of RNF8, amino acids 1 to 111

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    ring finger protein 8 (RNF8 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    containing only N-terminus amino acids 1 to 111

    Promoter

    CMV

    Available Since

    May 21, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pRevTRE MKL1 S449A/T450A/S454A

    Plasmid

    #19847

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 S449A/T450A/S454A (MRTFA Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Mutation

    changed Serine 449, Threonine 450 and Serine 454 …

    Available Since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

18. tet-pLKO shMKP5.v2 puro

    Plasmid

    #87793

    Purpose

    Expresses an inducible short hairpin targeting human MKP5 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shMKP5 (DUSP10 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE tight

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. tet-pLKO shMKP5.v1 puro

    Plasmid

    #87792

    Purpose

    Expresses an inducible short hairpin targeting human MKP5 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shMKP5 (DUSP10 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE tight

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAR-9.47_VP1,3

    Plasmid

    #74240

    Purpose

    Original plasmid described in Choudhury et al. 2015 Mol Ther. for production of AAV-AS vectors. Encodes VP1 and VP3 of AAV9.47

    Depositor

    Miguel Sena-Esteves

    Article

    Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

    Inserts

    AAV2 Rep
    AAV9.47 VP1
    AAV9.47 VP3
    

    Use

    AAV

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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