163,354 results

1. pCAG-CFP-SaCas9-HF (without sgRNA)

   Plasmid

   #134470

   Purpose

   Human expression vector for SaCas9-HF variant.

   Depositor

   Zongli Zheng

   Article

   Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. p

   Insert

   Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
   

   Use

   CRISPR

   Tags

   SV40NLS-3xFLAG

   Expression

   Mammalian

   Mutation

   R245A, N413A, N419A, R654A

   Promoter

   CAG

   Available since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 6691 Bicistronic_ires_puro

   Plasmid

   #64335

   Purpose

   This a retroviral expression plasmid with a minimal polylinker followed by IRES and puro resistance gene

   Depositor

   Georgios Stathopoulos

   Article

   Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pB1H2w2-zif268

   Plasmid

   #18045

   Depositor

   Scot Wolfe

   Article

   Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

   Insert

   zif268
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Zif268 is fused to the omega subunit of RNA polym…

   Promoter

   Available since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1-3'TwStrep

   Plasmid

   #208618

   Purpose

   Empty vector for mammalian expression, insert Twin Strep tag using BamHI and AflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with Twin Strep tag

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Use

   Tags

   Twin Strep

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pmCherryC1 Human WIP

   Plasmid

   #29573

   Depositor

   Anna Huttenlocher

   Article

   Cortesio et al Mol Biol Cell. 2010 Jan 1. 21(1):186-97.

   Insert

   WASP-interacting protein (WIPF1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Myc-RILPL1

   Plasmid

   #201470

   Purpose

   For overexpression in cultured cells to visualize RILPL1 protein interactions.

   Depositor

   Suzanne Pfeffer

   Article

   Pal et al Sci Adv. 2023 Dec 15;9(50):eadj1205. doi

   Insert

   RILPL1 (RILPL1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. N1-mSin1.1-GFP

   Plasmid

   #72907

   Purpose

   GFP-tagged mSin1 (isoform 1)

   Depositor

   Ivan Yudushkin

   Article

   Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

   Insert

   mSin1.1 (MAPKAP1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pmCellFree_KA1_SARS-COV-2_S

   Plasmid

   #169402

   Purpose

   Cell-free/mammalian expression vector for SARS-COV-2 virus S with N-term eGFP

   Depositor

   Kirill Alexandrov

   Insert

   SARS-COV-2 _S (S SARS-Cov2)
   

   Use

   Cell-free expression vector

   Tags

   His and eGFP

   Expression

   Mutation

   Promoter

   T7- CMV/CAG

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIB184-Km

   Plasmid

   #90195

   Purpose

   E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

   Depositor

   Indranil Biswas

   Article

   Hossain et al J Bacteriol. 2012 Jul;194(13):3486-94. d

   Type

   Empty backbone

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Flag-FOXC2

    Plasmid

    #153115

    Purpose

    Expression of Flag tagged human FOXC2 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXC2 (FOXC2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge