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pAV0757

Plasmid

#133509

Purpose

Expression of 3GFP tagged CRIB domain to monitor active Cdc42

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pHis5(StuI)-p(pak1)-CRIB(gic2aa2-181)-3GFP-terminator(ScADH1)-kanMX

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCRU5HT1-inLuc

Plasmid

#58954

Purpose

HTLV-1 transfer vector that encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is transduced only after the completion of viral cycle replication.

Depositor

David Derse, Dmitriy Mazurov

Article

Mazurov et al PLoS Pathog. 2010 Feb 26;6(2):e1000788.

Insert

inLuc

Use

Retroviral

Promoter

CMV/R/U5

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pMRX-INU-TEX264-FLAG

Plasmid

#128258

Purpose

Expresses TEX264 in mammalian cells

Depositor

Noboru Mizushima

Article

Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

Insert

TEX264 (TEX264 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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Pprm1-luciferase; his-3

Plasmid

#137728

Purpose

Communication-activated luciferase in Neurospora crassa

Depositor

Louise Glass

Article

Fischer et al Genetics. 2019 Apr;211(4):1255-1267. doi

Inserts

Luciferase
Pprm-1 Promoter

Use

Luciferase; Neurospora crassa expression

Mutation

Codon-optimized for N. crassa

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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CRY2high-mCherry

Plasmid

#104063

Purpose

Expresses fusion of CRY2high mutant (CRY2PHR E490R) with mCherry

Depositor

Bianxiao Cui

Article

Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

Insert

CRY2high-mCherry

Expression

Mammalian

Mutation

CRY2PHR E490R

Promoter

CMV

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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Plas-gRNA-Z3EV

Plasmid

#157659

Purpose

GFP gene and gRNA under Z3EV expression control (estradiol sensor), with Z3EV transcription factor to insert in the genome.

Depositor

Frank Albert

Article

Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

Insert

GFP-gRNA NatMX Z3EV

Use

Insert storage (replicative in e. coli)

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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6x-His_A1aY1_GFP

Plasmid

#158753

Purpose

Bacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purification

Depositor

Minhajuddin Sirajuddin

Article

Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

Insert

6xHistidine_A1aY1_GFP

Tags

6x-Histidine tag, GFP, T7 leader sequence, and Th…

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pMRX-INU-FLAG-FAM134Bs

Plasmid

#128261

Purpose

Expresses FAM134Bs in mammalian cells

Depositor

Noboru Mizushima

Article

Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

Insert

FAM134Bs (RETREG1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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G555

Plasmid

#216483

Purpose

Expression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.

Depositor

Christophe Danelon

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

plsB, plsC, cdsA, pssA

Use

Cell-free expression

Promoter

T7 for each gene

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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CRY2low-tdTomato

Plasmid

#104067

Purpose

Expresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with tdTomato

Depositor

Bianxiao Cui

Article

Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

Insert

CRY2low-tdTomato

Expression

Mammalian

Mutation

CRY2 aa 1-491 truncation, R489E A491D

Promoter

CMV

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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