We narrowed to 10,454 results for: ESP

1. pLV-mScarlet-SARS-CoV-2-5'UTRΔSL1

   Plasmid

   #191484

   Purpose

   Lentiviral expression of SARS-CoV-2 5' UTR ΔSL1

   Depositor

   Hao Wu

   Article

   Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

   Insert

   SARS-CoV-2 5' UTR ΔSL1 (NEWENTRY Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDN-G1Gh

   Plasmid

   #44507

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

   Inserts

   PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
   yEGFP
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Expression

   Yeast

   Mutation

   Two tandem tet operators (2XtetO2) downstream of …

   Promoter

   PGal1-D12 promoter

   Available Since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. mEos3.2trCD4o

   Plasmid

   #203751

   Purpose

   Encodes the transmembrane domain from CD4, including its 2 palmitoylation sites, with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   trCD4o (CD4 Human)
   

   Tags

   mEos3.2

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-tight-DDdCas9VPP300-T2A-GFP-IRES-Neo

   Plasmid

   #102891

   Purpose

   PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-P300-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE-tight

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5FRT/TO-YOD1-Strep-HA

   Plasmid

   #113483

   Purpose

   Expression of human YOD1 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   YOD1 (YOD1 Human)
   

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. tet-pLKO shMKP1.v2 puro

   Plasmid

   #87791

   Purpose

   Expresses an inducible short hairpin targeting human MKP1 sequence

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   shMKP1 (DUSP1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE tight

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330A-Yap1-E1-#1

   Plasmid

   #171517

   Purpose

   deletion of a genomic locus in Yap1 gene

   Depositor

   Hans Schöler

   Article

   Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

   Insert

   Yap1 (Yap1 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330A-Yap1-E2-#1

   Plasmid

   #171518

   Purpose

   deletion of a genomic locus in Yap1 gene

   Depositor

   Hans Schöler

   Article

   Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

   Insert

   Yap1 (Yap1 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-FRT/TO-neo-C11orf51-IRES2-mRuby (2763)

   Plasmid

   #39858

   Depositor

   Jonathon Pines

   Article

   Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

   Insert

   C11orf51 (ANAPC15 Human)
   

   Tags

   IRES2-mRuby

   Expression

   Mammalian

   Available Since

   Oct. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

10. mEos3.2trLAT-2P

    Plasmid

    #203748

    Purpose

    Encodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. Palmitoylation sites at C27 and C30 mutated to A. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trLAToC27AC30A (Lat Mouse)
    

    Tags

    ER translocation signal - mEos3.2

    Expression

    Mammalian

    Mutation

    C27A, C30A

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3 Flag MFF iso5 WT

    Plasmid

    #74378

    Purpose

    Flag tagged human MFF isoform 5

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pWay21-MKP-1 ASA

    Plasmid

    #13479

    Depositor

    Anton Bennett

    Article

    Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

    Insert

    MKP-1ASA (Dusp1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed Arginine 53 to Alanine, Changed Arginine…

    Available Since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pMSCV-puro-AS9 (Mito MTS)-Delta N67-mMCL1

    Plasmid

    #45822

    Depositor

    Joseph Opferman

    Article

    Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

    Insert

    AS9(Mito MTS)-Delta N67-mMCL1
    

    Expression

    Mammalian

    Mutation

    ATP Synthase subunit 9 is used as a Mito Targetin…

    Available Since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pEGFP-RNF8-C-terminus

    Plasmid

    #64673

    Purpose

    expresses only C terminus of RNF8, amino acids 402-485

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    Ring finger protein 8 (RNF8 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    containing only C-terminus amino acids 402 to 485

    Promoter

    CMV

    Available Since

    May 21, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pDual-eGFP(H66)

    Plasmid

    #63558

    Purpose

    Expression of the Azure (blue) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

    Depositor

    Richard S Myers

    Article

    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

    Insert

    His-T7-eGFP(H66)
    

    Use

    Lentiviral

    Tags

    His6 and T7

    Expression

    Bacterial and Mammalian

    Mutation

    Changed tyrosine at position 66 with respect to t…

    Promoter

    CMV-EF1α hybrid (CEF)

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

16. TRS6E1b-luc(-732/-721) mutant 5

    Plasmid

    #45388

    Depositor

    Thomas Gelehrter

    Article

    Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

    Insert

    6 copies of hPAI-1 promoter -732/-721 mutated at -725/723
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    -725/-723 mutated from gtt to ccc

    Available Since

    June 20, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pRD408

    Plasmid

    #178199

    Purpose

    Expression of H-NS from its natural promoter

    Depositor

    Remus Dame

    Article

    Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

    Insert

    phns + hns (hns Escherichia coli)
    

    Expression

    Bacterial

    Promoter

    hns promoter

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Foxf2 pcDNA HisC

    Plasmid

    #122121

    Purpose

    Expression of Foxf2 in mammalian cells

    Depositor

    Paul Herring

    Article

    Hoggatt et al J Biol Chem. 2013 Oct 4;288(40):28477-87

    Insert

    Foxf2 (Foxf2 Mouse)
    

    Tags

    6xHis and Express

    Expression

    Mammalian

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETDuet-1-6xHis-TEV-Nsp8

    Plasmid

    #201022

    Purpose

    Bacterial expression of codon optimized SARS-CoV-2 nsp8 tagged with an N-terminus His-tag followed by a TEV protease cleavage site.

    Depositor

    Sharon Rozovsky

    Article

    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

    Insert

    non-structural protein 8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-2TK cyclin T1 (1-250) (P#467)

    Plasmid

    #14606

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

    Insert

    cyclin T1 (1-250) (CCNT1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 1-250

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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