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Plasmid#159724PurposeExpress MPGIL-G6-Dhfr in yeast two hybrid plasmid, pGBKCgDepositorInsertmDHFR (Dhfr Mouse)
UseTagsGal4DBD and MPGIL-GGGGGGExpressionYeastMutationremove start and stop codon of DhfrPromoterADH1Available sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM1674
Plasmid#159726PurposeExpress MPTLV-G6-Dhfr in yeast two hybrid plasmid, pGBKCgDepositorInsertmDHFR (Dhfr Mouse)
UseTagsGal4DBD and MPTLV-GGGGGGExpressionYeastMutationremove start and stop codon of DhfrPromoterADH1Available sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM1479a3
Plasmid#159725PurposeExpress MYITV-G6-Dhfr in yeast two hybrid plasmid, pGBKCgDepositorInsertmDHFR (Dhfr Mouse)
UseTagsGal4DBD and MYITV-GGGGGGExpressionYeastMutationremove start and stop codon of DhfrPromoterADH1Available sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJB02
Plasmid#111959PurposeExpresses GST-GFP-DHFR; construct generated for pull-down and localization experimentsDepositorInsertsmEGFP
eDHFR
UseTagsDHFRExpressionBacterialMutationeDHFR;residues 2-159 and enhanced GFP with monome…PromoterT7Available sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJB03
Plasmid#111961PurposeExpresses MBP-GFP-DHFR; construct generated for pull-down and localization experimentsDepositorInsertsmEGFP
eDHFR
UseTagsDHFRExpressionBacterialMutationeDHFR;residues 2-159 and enhanced GFP with monome…PromoterTacAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRT029
Plasmid#127969Purposedouble-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locusDepositorInsertCLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
UseTALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDBD-M
Plasmid#50572PurposeDouble Ub M-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50571DepositorInsertDHFR-Ubiquitin-MetBetaGal-Ubiquitin-DHFR-Ubiquitin
UseTagsExpressionYeastMutationPromoterGal1Available sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDBD-R
Plasmid#50571PurposeDouble Ub R-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50572DepositorInsertDHFR-Ubiquitin-ArgBetaGal-Ubiquitin-DHFR-Ubiquitin
UseTagsExpressionYeastMutationM1R (B-gal)PromoterGal1Available sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pERB215
Plasmid#61499PurposeConst. Halotag-GFP-Nuf2 + mCherry-DHFRDepositorInsertsHaloTag-GFP-Nuf2
mCherry-DHFR
UseTagsHaloTag and mCherryExpressionMammalianMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pERB221
Plasmid#61502Purposeconst. CenpB-GFP-Halo + mCherry-DHFRDepositorInsertsCenpB-GFP-Halo
mCherry-DHFR
UseTagsHaloTag and mCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only