We narrowed to 716 results for: Bacillus
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Plasmid#210872PurposeExpresses GstTnpB3 and native ωRNA from constitutive promotersDepositorInsertGstTnpB3
ExpressionBacterialAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFE-dabAB2
Plasmid#133002PurposepFE carrying both the dabA2 (Uniprot: D0KWS7) and dabB2 genes (Uniprot: D0KWS8)DepositorInsertsdabB2
dabA2
ExpressionBacterialPromotertetRAvailable SinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF1-Pls1-sfGFP
Plasmid#205748PurposeExpresses sfGFP under IPTG inducible promoter Pls1 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Low strength.DepositorTypeEmpty backboneUseShuttle vector gram+ gram-ExpressionBacterialPromoterPls1 (Phyperspank mutant, IPTG inducible)Available SinceAug. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292 (AaCas12b)-D570A-TV
Plasmid#136380PurposeCRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation systemDepositorInsertAaCas12b (D570A)-TV
UseCRISPRExpressionPlantMutationD570AAvailable SinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD02-SsMenshen
Plasmid#254577PurposeFor the expression of the Menshen system encoded by Solibacillus silvestris StLB046DepositorInsertSsNsnA, SsNsnB, SsNsnC
ExpressionBacterialAvailable SinceMay 1, 2026AvailabilityAcademic Institutions and Nonprofits only -
pBlu-CGT-1
Plasmid#170158PurposeExpresses bacterial Cyclomaltodextrin Glucanotransferase from Paenibacillus sp. c36 in E.coliDepositorInsertCyclomaltodextrin Glucanotransferase
ExpressionBacterialPromoterTacAvailable SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ290-D570A
Plasmid#129673PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAacCas12b
UseCRISPR; Gateway compatible aaccas12b-d570a entry …ExpressionPlantMutationD570A; AacCas12b is rice codon optimizedAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292-D570A-SRDX
Plasmid#129684PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAaCas12b-SRDX
UseCRISPR; Gateway compatible aacas12b entry cloneExpressionPlantMutationD570A; AaCas12b is rice codon optimizedAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTCas9_scr
Plasmid#207566PurposeThermoCas9-mediated cleavage of genomic DNA in E. coliDepositorInsertPlac/tet_ThermoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)
ExpressionBacterialMutationWild-typeAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
enDR3-TEV-mEGFP-NLS (pRS1451)
Plasmid#240546PurposeExpression of the engineered N-terminal hybrid-binding domain of RNase H3 (enDR3) for capturing R-loops. A TEV cleavage site, mEGFP and nuclear localization signal (NLS) are included in the fusion.DepositorInsertenDR3
ExpressionMammalianMutationL52MAvailable SinceAug. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLz74_Rubisco_CsoS2_Npeptide_fusion
Plasmid#140840PurposeSame as pBz15 but with N*-peptide fusion on CbbL, construct used for protein crystallizationDepositorInsertForm IA Rubisco with CsoS2 N-peptide fusion; CbbL-Npeptide, CbbS
TagsCsoS2 N-peptide consensus to CbbL C-terminus and …ExpressionBacterialPromoterT7Available SinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
Catalytically Enhanced (E655G and N696I) -ThermoCas9-3xNLS
Plasmid#254684PurposeExpresses Catalytically Enhanced (E655G and N696I) ThermoCas9 with 3x NLS tags. Utilized for RNP transfectionDepositorInsertCatalytically Enhanced (E655G and N696I) ThermoCas9
TagsNLS, SV40 NLS, and nucleoplasmin NLSExpressionBacterialAvailable SinceMay 6, 2026AvailabilityAcademic Institutions and Nonprofits only -
nb-enDR3-TEV-mEGFP-NLS (pRS1458)
Plasmid#240547PurposeExpression of negative control for R-loops capture experiments using enDR3. A mutated, non-R-loops binding version of enDR3 is expressed in fusion with the TEV cleavage site, mEGFP, and NLS.DepositorInsertnb-enDR3
ExpressionMammalianMutationS48A, K50AAvailable SinceAug. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)_co_L-HicDH
Plasmid#185453PurposeCDS of L-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 in pET21a(+).DepositorInsertL-HicDH from Lactobacillus confusus DSM 20196
ExpressionBacterialPromoterT7Available SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEVA251_ Ptrc.x.tetO2::D-HicDH
Plasmid#185456PurposeCDS of D-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 under the control of the synthetic promoter Ptrc.x.tetO2 and the RBS BBa_B0030 in pSEVA251 plasmid.DepositorInsertD-HicDH from Lactobacillus paracasei DSM 20008
ExpressionBacterialPromoterPtrc.x.tetO2Available SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM7
Plasmid#201213PurposeIntegrative GFP-based shuttle vector for genetic engineering of Parageobacillus thermoglucosidasius. Carries homologous flanks for deletion of key sporulation regulator spo0A.DepositorInsertssfGFP
Spo0A right flank
Spo0A left flank
UseSynthetic BiologyExpressionBacterialMutationF11V, T13P, N39D, A179AAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGMβ1
Plasmid#216202PurposeChromosomal gene manipulation (gene insertion, conversion, deletion) of dairy used Lactobacillus bulgaricus, a difficult gene to manipulate, is now possible by conjugation using this pGMB1 plasmid.DepositorTypeEmpty backboneUseShuttle vector, conjugal plasmid, theta type rep…ExpressionBacterialPromoterlac promoter in pGEM-Teasy, Promoters in pAMbeta1…Available SinceJan. 21, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
LVIS_1595
Plasmid#255508PurposeDERA enzyme production under control of T7 promoter and inducible lac operatorDepositorInsert2-deoxyribose-5-phosphate aldolase (DERA) from Lactobacillus brevis
Tags6xHis tag-TEV protease recognition site (ENLYFQ/G…ExpressionBacterialMutationWTPromoterT7Available SinceMay 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pMZ7
Plasmid#223180PurposepORI28 derivative with lacZ and upp gene inserted into the backbone; for generating gene-specific knockouts.DepositorTypeEmpty backboneUseChromosomal integrationAvailable SinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
BlatCas9
Plasmid#163792PurposeThe plasmid expresses human codon-optimized BlatCas9 and gRNA expression elements.DepositorTypeEmpty backboneUseAAV and CRISPRExpressionMammalianAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only