Addgene

Showing: 601 - 620 of 761 results

Results per page:

Sort results by:

pHD-mW-attPCC-FRT (pTL717)

Plasmid

#115158

Purpose

Vector for generating dsDNA donors for HDR. Contains an attP-CC docking site, the visible GMR-white for identifying backbone incorporation flanked by FRT sites for FLP-mediated removal.

Depositor

Tobias Langenhan

Article

Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.

Insert

mW attP-CC FRT

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBRC169

Plasmid

#79670

Purpose

DsREDmonomer-based BiFC vector for transient expression of RC (169-225) in plants.

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Type

Empty backbone

Use

Reporter plasmid

Tags

DsRED-monomer (169–225)/RC169

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBRN168

Plasmid

#79668

Purpose

DsREDmonomer-based BiFC vector for transient expression of RN (1-168) in plants.

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Type

Empty backbone

Use

Reporter plasmid

Tags

DsRED-monomer (1–168)/RN168

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
OA-986B

Plasmid

#124999

Purpose

Expresses dCas9-VPR driven by the Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

dCas9-VPR

Use

Piggyback

Tags

Expression

Insect

Mutation

Promoter

Ubiquitin

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pUASt-ssBiP-ESCargo

Plasmid

#140166

Purpose

Expresses a regulatable secretory cargo for Drosophila melanogaster

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

ssBip-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Use

Tags

Expression

Insect

Mutation

Promoter

hs70

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDSARN

Plasmid

#62295

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, DsRed-NLS marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
p155ME-loxP-dsR-loxP-Cas9-t2A-GFP

Plasmid

#227774

Purpose

Middle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloning

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Insert

loxP-dsR-loxP

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
OA-986C

Plasmid

#125000

Purpose

Expresses dCas9-VPR driven by the bottleneck promoter

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

dCas9-VPR

Use

Piggyback

Tags

Expression

Insect

Mutation

Promoter

bottleneck

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
OA-986D

Plasmid

#125001

Purpose

Expresses dCas9-VP64 driven by the Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

dCas9-VP64

Use

Piggyback

Tags

Expression

Insect

Mutation

Promoter

ubiquitin

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
OA-986E

Plasmid

#125002

Purpose

Expresses dCas9-VP64 driven by the bottleneck promoter

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

dCas9-VP64

Use

Piggyback

Tags

Expression

Insect

Mutation

Promoter

bottleneck

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-fSST-RFP

Plasmid

#22913

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

fugu somatostatin promoter driving DsRed 2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-KS-attB1-2-PT-SA-SD-0-2xTY1-V5

Plasmid

#61255

Purpose

CRISPR-RMCE, second step attB plasmid, intron phase 0, exchange dsRed with 2xTY1 and V5 tags cassette

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Insert

2xTY1-V5

Use

CRISPR

Tags

TY1 and V5

Expression

Mutation

Promoter

Available since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRES RED/SP M87C448Y

Plasmid

#89324

Purpose

Expression of RFP and M87C448Y spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-460 deleted

Promoter

CMV

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Ubiq-Cas9.874W

Plasmid

#112686

Purpose

Express hSpCas9 under Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

hSpCas9

Use

Tags

3x FLAG, EGFP, and NLS

Expression

Insect

Mutation

Humanized Cas9 bacterial sequence

Promoter

Ubiquitin-63E promoter

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
vas-Cas9.874Z

Plasmid

#112687

Purpose

Express hSpCas9 under vasa promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

hSpCas9

Use

Tags

3x FLAG, EGFP, and NLS

Expression

Insect

Mutation

Humanized Cas9 bacterial sequence

Promoter

vasa

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
nos-Cas9.874Z1

Plasmid

#112685

Purpose

Express hSpCas9 under nanos promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

hSpCas9

Use

Tags

3x FLAG, EGFP, and NLS

Expression

Insect

Mutation

Humanized Cas9 bacterial sequence

Promoter

nanos

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
RT3REVIR

Plasmid

#111168

Purpose

Tet-regulated miR-E (miR-30 variant)-based RNAi

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Type

Empty backbone

Use

Retroviral

Tags

dsRed and mVenus

Expression

Mutation

Promoter

T3G

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LT3REV

Plasmid

#111174

Purpose

Tet-regulated miR-E (miR-30 variant)-based RNAi

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Type

Empty backbone

Use

Lentiviral

Tags

DsRed and Venus

Expression

Mutation

Promoter

T3G

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-KS-attB1-2-PT-SA-SD-2-2xTY1-V5

Plasmid

#61257

Purpose

CRISPR-RMCE, second step attB plasmid, intron phase 2, exchange dsRed with 2xTY1 and V5 tags cassette

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Insert

2xTY1-V5

Use

CRISPR

Tags

TY1 and V5

Expression

Mutation

Promoter

Available since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
NCVB-ssGrl1-ESCargo*

Plasmid

#140167

Purpose

Expresses a regulatable secretory cargo (without an ER export signal) for Tetrahymena thermophila

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

ssGrl1-DsRed-Express2-FKBP(LV)(C22V)

Use

Other

Tags

Expression

Mutation

Promoter

MTT1

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge