Addgene

We narrowed to 1,215 results for: calcium

Showing: 601 - 620 of 1215 results

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pCAG-Synapto-pHluorin

Plasmid

#168510

Purpose

Expression of Synapto-pHluorin for optical monitoring of presynaptic release in neurons.

Depositor

Franck Polleux

Article

Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

Insert

Synaptophysin-pHluorin (Syp Mouse)

Use

Tags

pHluorin

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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Nrxn3b-Nav1.6-MKII-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16

Plasmid

#163029

Purpose

FLiCRE TF for neuronal expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

Nrxn3b-MKII-hLOV1-TEVcs-tTA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-RiboL1-jGCaMP8s

Plasmid

#192616

Purpose

AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8s.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

FLEX-RIboL1-jGCaMP8s

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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FMRP shRNA

Plasmid

#206074

Purpose

expression of FMRP RNA for gene silencing in mammalian cells

Depositor

Annette Dolphin

Article

Ferron et al Nat Commun. 2014 Apr 7;5:3628. doi: 10.1

Insert

FMRP (Fmr1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-RiboL1-jGCaMP8m

Plasmid

#192617

Purpose

AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

FLEX-RiboL1-jGCaMP8m

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Nrxn3b-Nav1.6-MKII-f-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16

Plasmid

#163031

Purpose

FLiCRE TF with fast kinetics for neuronal expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

Nrxn3b-MKII-f-hLOV1-TEVcs-tTA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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tetO-TBX5

Plasmid

#46032

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

TBX5 (TBX5 Human)

Use

Lentiviral

Tags

His and V5

Expression

Mammalian

Mutation

Promoter

tetO

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-ADP-MCS-FLAG

Plasmid

#192360

Purpose

Adipocyte-specific overexpression of genes

Depositor

Yifu Qiu

Article

Xue et al Cell Metab. 2022 Sep 6;34(9):1325-1341.e

Type

Empty backbone

Use

AAV

Tags

3xflag

Expression

Mammalian

Mutation

Promoter

adiponectin promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-RiboL1-jGCaMP8f

Plasmid

#192618

Purpose

AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8f.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

FLEX-RiboL1-jGCaMP8f

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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PH-PLCD1(R40L)-GFP

Plasmid

#51408

Purpose

Non-PI(4,5)P2 binding control for PH-PLCD1-GFP

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

PH-PLCD1 (aa 1–170) (PLCD1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Arginine 40 mutated to leucine

Promoter

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-RiboL1-jGCaMP7c

Plasmid

#192619

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted (RiboL1) jGCaMP7c.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP7c

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax

Plasmid

#173870

Purpose

Mammalian expression of mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax (opto-RhoA)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax

Use

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

Plasmid

#173872

Purpose

Mammalian expression of mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

Use

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Axon-jRex-GECO

Plasmid

#172714

Purpose

AAV transfer plasmid for neuronal expression of axon-targeted jRex-GECO.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Axon-jRex-GECO

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax

Plasmid

#173871

Purpose

Mammalian expression of mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax (opto-Rac1)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax

Use

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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CD4-MKII-f-hLOV1-TEVcs(ENLYFQ/M)- Gal4

Plasmid

#163027

Purpose

FLiCRE TF with fast kinetics for HEK293T expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

CD4-MKII-f-hLOV1-TEVcs-Gal4

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLYS5-SDHB-Flag

Plasmid

#50055

Purpose

Expression of human SDHB in mammalian cells

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

SDHB (SDHB Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pPBpuro-COX2-EBFP2

Plasmid

#184720

Purpose

Exogenous COX2

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

COX2-EBFP2 (PTGS2 Synthetic, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3

Plasmid

#112007

Purpose

For co-expression of GCaMP6s with mRuby3

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV9

Insert

GCaMP6s-P2A-mRuby3

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSynapsin1

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Htr5b(3.7)-tTA-sv40

Plasmid

#184412

Purpose

AAV expression of tTA from mouse 5-hydroxytryptamine (serotonin) receptor 5B (Htr5b) gene promoter.

Depositor

Marylka Yoe Uusisaari

Article

Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

Insert

Mouse Htr5b gene promoter-tTA

Use

AAV

Tags

tTA

Expression

Mammalian

Mutation

Promoter

Mouse Htr5b gene

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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