We narrowed to 1,631 results for: prep

1. pcDNA5-FRT/TO-N-mEGFP-PARP7

   Plasmid

   #178004

   Purpose

   Expresses N-terminally mEGFP-tagged PARP7

   Depositor

   Karla Feijs

   Article

   Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

   Insert

   PARP7 (TIPARP Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. FAM177A1isoform2-GFP

   Plasmid

   #224589

   Purpose

   For expression of FAM177A1-GFP in mammalian cells

   Depositor

   Pietro De Camilli

   Article

   Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

   Insert

   FAM177A1 (FAM177A1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAD-LyseR (in lacZ deficient strain)

   Plasmid

   #99245

   Purpose

   For autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)

   Depositor

   Jeff Hasty

   Article

   Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

   Insert

   phage lambda gene R (R phage lambda)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   beta-lactamase promoter

   Available since

   Oct. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. VPS13B^GFP

   Plasmid

   #224588

   Purpose

   codon optimized GFP tagged VPS13B for expression in mammalian cells

   Depositor

   Pietro De Camilli

   Article

   Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

   Insert

   VPS13B^GFP (VPS13B Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE

   Plasmid

   #223175

   Purpose

   For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

   Insert

   hU6_Rfxcas13d-DR1_BsmBI
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   DR1 A>T

   Promoter

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET30a-SARS-CoV2-NP

   Plasmid

   #165090

   Purpose

   protein expression in E. coli

   Depositor

   Rick Tarleton

   Article

   Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

   Insert

   Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal (N SARS-CoV2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. MBC2-null

   Plasmid

   #162749

   Purpose

   MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2) for expression of TCR genes

   Depositor

   Maki Nakayama

   Article

   Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

   Insert

   murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MBC1-null

   Plasmid

   #162748

   Purpose

   MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1) for expression of TCR genes

   Depositor

   Maki Nakayama

   Article

   Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

   Insert

   murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. WT USP7 catalytic

   Plasmid

   #227201

   Purpose

   Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Article

   Valles et al bioRxiv [Preprint]. 2023 Jan 12:2023.01.

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   aa 207-560

   Promoter

   T7-promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA5-FRT/TO-N-mEGFP-PARP6

    Plasmid

    #178005

    Purpose

    Expresses N-terminally mEGFP-tagged PARP6

    Depositor

    Karla Feijs

    Article

    Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

    Insert

    PARP6 (PARP6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5-FRT/TO-N-mEGFP-PARP15

    Plasmid

    #178000

    Purpose

    Expresses N-terminally mEGFP-tagged PARP15

    Depositor

    Karla Feijs

    Article

    Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

    Insert

    PARP15 (PARP15 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET30a-Spike-E

    Plasmid

    #165096

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 spike protein (S SARS-CoV2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 11, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pLentiRNACRISPR_008-TetO-NLS-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #223173

    Purpose

    Expresses hfCas13d (N2V8) in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus.

    Depositor

    Neville Sanjana

    Article

    Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

    Insert

    hfCas13d (N2V8)
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSpCas9-2A-Puro-TBK1-gRNA (PX459)

    Plasmid

    #221550

    Purpose

    Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human TBK1 (TBK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    u6

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MBC1-PuroIII

    Plasmid

    #153419

    Purpose

    MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1) for expression of TCR genes

    Depositor

    Maki Nakayama

    Article

    Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSBL_DK202

    Plasmid

    #156426

    Purpose

    Mammalian expression of 3a delta N(aa 42-275)-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells

    Depositor

    Stephen Brohawn

    Article

    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

    Insert

    SARS-CoV-2 3a protein (aa 42-275)
    

    Use

    Tags

    EGFP-His

    Expression

    Mammalian

    Mutation

    aa 42-275

    Promoter

    CMV

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET30a-SARS-CoV2-ORF8

    Plasmid

    #165089

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 ORF8 (ORF8 SARS-CoV2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. MBC2-PuroIII

    Plasmid

    #153420

    Purpose

    MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2) for expression of TCR genes

    Depositor

    Maki Nakayama

    Article

    Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ATP5F1B-turboGFP

    Plasmid

    #239795

    Purpose

    Expresses human ATP5F1B tagged with turboGFP

    Depositor

    Samantha Lewis

    Article

    Rose et al bioRxiv [Preprint]. 2025 Mar 25:2025.03.

    Insert

    ATP5F1b (ATP5F1B Human)
    

    Use

    Tags

    turboGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRCA905 - KLF4 Puro-T2A-BFP (pRCA847 backbone)

    Plasmid

    #238185

    Purpose

    Lentiviral vector for overexpression KLF4 ORF from a EF1a promoter

    Depositor

    Thomas Norman

    Article

    Southard et al bioRxiv [Preprint]. 2024 Aug 3:2024.07.3

    Insert

    KLF4 (KLF4 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge