We narrowed to 11,691 results for: NSI

1. pMSCV-Flag-hOct4

   Plasmid

   #20072

   Depositor

   Juan Belmonte

   Article

   Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

   Insert

   hOct4 (POU5F1 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Jan. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-trGluc

   Plasmid

   #158959

   Purpose

   DNA donor plasmid containing repair template for Gaussia luciferase (Gluc) of bioluminescent repair reporter (BLRR).

   Depositor

   Charles P. Lai

   Article

   Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

   Insert

   trGluc
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEXtev-HMG54kd

   Plasmid

   #26030

   Depositor

   Johann Deisenhofer

   Article

   Istvan et al EMBO J. 2000 Mar 1. 19(5):819-30.

   Insert

   3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Catalytic domain (amino acids 426-488) Methion…

   Available Since

   Dec. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. His-SUMO-hDDI2_pET11a

   Plasmid

   #169120

   Purpose

   expression of protein

   Depositor

   Barbara Dirac-Svejstrup

   Article

   Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

   Insert

   hDDI (DDI2 Human)
   

   Expression

   Bacterial

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. LifeAct-14 (LA-14)

   Plasmid

   #158750

   Purpose

   Lentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.

   Depositor

   Minhajuddin Sirajuddin

   Article

   Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152

   Insert

   LifeAct-14
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Deletion mutation for last three amino acids from…

   Promoter

   CMV immediate early

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Lyso-ExRai-AktAR2

   Plasmid

   #184052

   Purpose

   Lysosome-targeted ExRai-AktAR2.

   Depositor

   Jin Zhang

   Article

   Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

   Insert

   Lyso-ExRai-AktAR2
   

   Tags

   Lysosome-associated membrane protein 1 (LAMP1).

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiCRISPR v2-sgMyc_1

   Plasmid

   #138324

   Purpose

   Express guide RNA 2 for mouse Myc

   Depositor

   Yi Zhang

   Article

   Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

   Insert

   cMyc sgRNA (Myc Synthetic)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCh-LgBiT-2A-EGFP-Vin-PILATeS

   Plasmid

   #216761

   Purpose

   PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS (700 pM) in mammalian cells. Requires transposase

   Depositor

   Alex Dunn

   Article

   Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

   Insert

   mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS
   

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Available Since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFP5370 ss-FIREmate-HA- KDEL-IVS-IRES- TNF-SBP- mCherry-FIREtag

   Plasmid

   #216696

   Purpose

   Synchronize trafficking of TNFalpha from the ER (RUCH system)

   Depositor

   Franck Perez

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Inserts

   FIREmate-KDEL
   Tumor Necrosis Factor alpha (TNFa)
   

   Tags

   FIREtag, Streptavidin Binding Protein (SBP), and …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX SH3(2)-4R

    Plasmid

    #112090

    Purpose

    Bacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    SH3(2)-4R (NCK1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3xFLAG-dCas9/pMXs-puro

    Plasmid

    #51240

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available Since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. tdp43-EGFP construct3

    Plasmid

    #28196

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa170-414 only

    Available Since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pDuet TDP-43 M337V

    Plasmid

    #27464

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Coexpression

    Expression

    Bacterial

    Mutation

    Methionine 337 mutated to Valine (M337V)

    Available Since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

14. mEGFP-HRas S17N

    Plasmid

    #18665

    Depositor

    Karel Svoboda

    Article

    Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

    Insert

    H-Ras (HRAS Human)
    

    Tags

    monomeric EGFP

    Expression

    Mammalian

    Mutation

    S17N mutation in H-Ras

    Available Since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiCRISPR v2-sgMyc_2

    Plasmid

    #138346

    Purpose

    Express guide RNA 1 for mouse Myc

    Depositor

    Yi Zhang

    Article

    Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

    Insert

    cMyc sgRNA (Myc Synthetic)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDV21_feedback_entry_TagBFP-2A-MCS-2A-NLuc-2A-MCP-ADAR2dd(E488Q)-NES

    Plasmid

    #200403

    Purpose

    Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses NanoLuc and MCP-ADAR upon target detection

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    Closed-loop DART VADAR sensor entry cassette
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCold TDP43 M337V

    Plasmid

    #27453

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    Factor Xa and His6

    Expression

    Bacterial

    Mutation

    Methionine 337 mutated to Valine (M337V)

    Available Since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pCMV7.1_3xFLAG-TAOK1

    Plasmid

    #172991

    Purpose

    constitutive expression of FLAG-tagged TAOK1

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-TAOK1 (TAOK1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-MLC2(CA)-IRES-GFP

    Plasmid

    #133928

    Purpose

    Bicistronic vector for the expression of EGFP reporter and untagged constitutive active myosin II light chain

    Depositor

    Frank Bradke, Sebastián Dupraz

    Article

    Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

    Insert

    EGFP and MLC2(CA) (MYL2 Human)
    

    Expression

    Mammalian

    Mutation

    MLC2 T18D, S19D

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. PAmCherry1-C1-LACT-C2

    Plasmid

    #202682

    Purpose

    Photoactivatable lipid biosensor

    Depositor

    Gerry Hammond

    Article

    Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

    Insert

    PAmCherry1: Bos taurus MFGE8 (274-431) (MFGE8 Bovine)
    

    Expression

    Mammalian

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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