We narrowed to 9,173 results for: Pol

1. pTU1-A-pdh_RiboJ_mCherry_Bba_B0015

   Plasmid

   #107581

   Purpose

   B. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

   Depositor

   Paul Freemont

   Article

   Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pdh promoter Bacillus megaterium DSM319

   Available Since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. SaCas9 GFP V3

   Plasmid

   #226962

   Purpose

   CBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRE-Lepr_FL-IRES-eGFP

   Plasmid

   #196635

   Purpose

   Doxycycline inducible expression of full length mouse Lepr with eGFP marker

   Depositor

   Elaine Fuchs

   Article

   Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

   Insert

   Lepr (Lepr Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZac2.1-GfaABC1D-mCherry-hPMCA2w/b

   Plasmid

   #111568

   Purpose

   Expresses a plasma membrane calcium pump hPMCA2w/b

   Depositor

   Baljit Khakh

   Article

   Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

   Has Service

   AAV5

   Insert

   Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
   

   Use

   AAV

   Tags

   mCherry

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. PDG459 V3

   Plasmid

   #226958

   Purpose

   CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. peGFP C3-SPG20 8xV

   Plasmid

   #218579

   Purpose

   Expresses GFP-tagged human SPG20 bulky hydrophobic amino acid mutant in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

   Insert

   SPG20 (SPART Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   changed Tryptophan 419, Leucine 438, Isoleucine 4…

   Promoter

   CMV

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Frt-hspB3

   Plasmid

   #63094

   Purpose

   Expression of HSPB3 (small HSP) in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb3 (HSPB3 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. EBFP2-UtrCH

   Plasmid

   #124656

   Purpose

   F-actin marker. EBFP2 is fused to the calponin homology domain of utrophin (UtrCH).

   Depositor

   Cheng-han Yu

   Article

   Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

   Insert

   Utrophin (UTRN Human)
   

   Tags

   EBFP2

   Expression

   Mammalian

   Mutation

   see depositor comments below

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. SaCas9v3-Puro

   Plasmid

   #178813

   Purpose

   SaCas9 with 2A-Puro, and a cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Cbh (Cas9-2A Puro) and U6 (gRNA)

   Available Since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pN094

    Plasmid

    #167155

    Purpose

    MoClo-compatible Level1 (position 6) vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA under control of 0.4 kb 35S promoter, for expression in plants

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    p35s-npgA-ocsT
    

    Expression

    Plant

    Promoter

    p35s

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLIB-HIS-TEV-CKS1

    Plasmid

    #177013

    Purpose

    Generate baculovirus for insect cell expression of CKS1 with N-terminal Polyhistidine-TEV

    Depositor

    Andrea Musacchio

    Article

    Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

    Insert

    CKS1 (CKS1B Human, Synthetic)
    

    Tags

    Polyhistidine-TEV

    Expression

    Insect

    Mutation

    codon-optimised for insect cell expression

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. HaloTag-LaminA

    Plasmid

    #231894

    Purpose

    Expression of HaloTag-tagged LaminA to visualize the nuclear lamina

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    LaminA (LMNA Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmCherry-N1-EXO1b

    Plasmid

    #111621

    Purpose

    Expression of human WT Exonuclease 1 (EXO1) tagged with mCherry in mammalian cells

    Depositor

    Marco Muzi-Falconi

    Article

    Sertic et al Mol Cell. 2018 Apr 5;70(1):34-47.e4. doi

    Insert

    Exonuclease 1b (EXO1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZac2.1 GfaABC1D hM3D mCherry SV40

    Plasmid

    #92284

    Purpose

    AAV DREADD expression

    Depositor

    Baljit Khakh

    Article

    Chai et al Neuron. 2017 Aug 2;95(3):531-549.e9. doi

    Insert

    hM3D (CHRM3 Human)
    

    Use

    AAV

    Tags

    mCherry

    Promoter

    GfaABC1D

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV6-AN-DDK_WT RAD23A

    Plasmid

    #201438

    Purpose

    Expresses human RAD23A with an N-terminal FLAG tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET32a-HD46Q

    Plasmid

    #11515

    Depositor

    Pamela Bjorkman

    Article

    Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

    Insert

    huntingtin, exon 1, 46Q glutamines (HTT Human)
    

    Tags

    His, His (out of frame), and Trx

    Expression

    Bacterial

    Mutation

    huntingtin exon 1 with 46 glutamines, vector m…

    Available Since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP Cep170 (Nigg PID200)

    Plasmid

    #41150

    Depositor

    Erich Nigg

    Article

    Guarguaglini et al Mol Biol Cell. 2005 Mar;16(3):1095-107.

    Insert

    Cep170 (CEP170 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-UbC

    Plasmid

    #62806

    Purpose

    To express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.

    Depositor

    Jae Lee, Pantelis Tsoulfas

    Article

    Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

    Type

    Empty backbone

    Use

    AAV; Eucaryotic

    Expression

    Mammalian

    Promoter

    hUbC

    Available Since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. ABEmax-Puro V3

    Plasmid

    #226956

    Purpose

    ABEmax plasmid enabling A:T to G:C base editing using a single plasmid. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCI-MS2V5-eIF4G delN83

    Plasmid

    #65812

    Purpose

    expression of eIF4G-MS2 fusion.

    Depositor

    Niels Gehring

    Article

    Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

    Insert

    eIF4G (EIF4G1 Human)
    

    Tags

    MS2 stem loops and V5

    Expression

    Mammalian

    Mutation

    delN83 (functionally behaves as the WT protein)

    Promoter

    CMV

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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