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pMKO.1-shEsco2

Plasmid

#160957

Purpose

Esco2 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Esco2 shRNA (Esco2 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe-ER-E2F2-dRb

Plasmid

#62805

Purpose

expresses an inducible E2F2 mutant that lacks the Rb binding domain

Depositor

Gerardo Ferbeyre

Article

Vernier et al Genes Dev. 2011 Jan 1;25(1):41-50. doi:

Insert

E2F2 (E2F2 Human)

Use

Retroviral

Tags

HA-Er

Expression

Mammalian

Mutation

deletion of amino acids 302-437, including the Rb…

Promoter

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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HOXB1 (human) HIS-tag pET

Plasmid

#8520

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXB1 (HOXB1 Human)

Use

Tags

His and T7

Expression

Bacterial

Mutation

PCR from cDNA clone

Promoter

Available since

July 6, 2006

Availability

Academic Institutions and Nonprofits only
pLVX-EF1a-EGFP-TA Bik-IRES-Puromycin

Plasmid

#133026

Purpose

Expresses EGFP-tagged tail anchor sequence of Bik

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Bcl-2-interacting killer (BIK Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-110

Promoter

Human elongation factor 1 alpha

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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p2Flag CMV2-ZO-2-N

Plasmid

#27418

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

Deletion of the carboxy-terminus of ZO-2, so only…

Promoter

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only
pFLAG-4S Tetherin

Plasmid

#41073

Depositor

Paul Spearman

Article

Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

Insert

Tetherin (BST2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S74, S84, 137S, and 144S

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pMKO.1-shBub1

Plasmid

#160948

Purpose

Bub1 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Bub1 shRNA (Bub1 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBB0323-TEV-His12

Plasmid

#137039

Purpose

expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0323 with C-terminal TEV-protease-cleavable His12

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Insert

AAC66700.1

Use

Tags

His12 (TEV protease cleavable)

Expression

Bacterial

Mutation

wild type, full-length protein, contains signal p…

Promoter

T7

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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HS-DN-Cep152-mKate2

Plasmid

#105943

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

Cep152 (CEP152 Human)

Use

Tags

mKate2

Expression

Bacterial

Mutation

dominant negative (AA 1-512)

Promoter

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCdk1

Plasmid

#160953

Purpose

Cdk1 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Cdk1 shRNA (Cdk1 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pFLAG-137.144S Tetherin

Plasmid

#41072

Depositor

Paul Spearman

Article

Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

Insert

Tetherin (BST2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

137S, 144S

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
MAP1LC3A-V1

Plasmid

#98569

Purpose

Expresses MAP1LC3A-V1

Depositor

Shahenda El-Naggar

Article

Nassar et al Sci Rep. 2017 Aug 14;7(1):8022. doi: 10.

Insert

MAP1LC3A (MAP1LC3A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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API5-CRISPR

Plasmid

#157664

Purpose

Expresses Cas9 and sgRNA targeting API5

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Apoptosis inhibitor 5 (API5 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-α-COPI-WD40

Plasmid

#180758

Purpose

Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 (residues 1-327)

Depositor

Syed Saif Hasan

Article

Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

Insert

α-COPI-WD40

Use

Tags

C-terminal strep tag

Expression

Mammalian

Mutation

Encodes residues 1-327; mutations: Leu181Lys, Leu…

Promoter

CMV

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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HOXB3 (human) HIS-tag pET

Plasmid

#8524

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXB3 (HOXB3 Human)

Use

Tags

His and T7

Expression

Bacterial

Mutation

Contains 5 additional amino acids before ATG from…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1L16A/L17A

Plasmid

#13478

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1L16A/L17A (Dusp1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Changed Leucine 16 to Alanine; changed Leucine 1…

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pLV-EF1a-V5-Arl6ip6

Plasmid

#120235

Purpose

Expresses V5-tagged Arl6ip6 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Arl6ip6 (Arl6ip6 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PARL-FLAG-CT H335G

Plasmid

#13749

Depositor

Luca Pellegrini

Article

Cipolat et al Cell. 2006 Jul 14. 126(1):163-75.

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Changed catalytic His 335 to Gly

Promoter

Available since

Feb. 1, 2007

Availability

Academic Institutions and Nonprofits only
GFP-Drosha218-1374

Plasmid

#62523

Purpose

N-terminal aa1-217 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 1-217 deleted (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-217

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
GFP-Drosha270-1374

Plasmid

#62524

Purpose

N-terminal aa1-269 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 1-269 deleted (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-269

Promoter

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only