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pXN22-Dest

Plasmid

#105085

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

Depositor

Christopher Grefen

Publication

Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

Type

Empty backbone

Tags

NubG-3xHA

Expression

Yeast

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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YIPlac204TKC-DsRed-Express2-HDEL

Plasmid

#21770

Depositor

Benjamin Glick

Insert

DsRed-Express2

Tags

HDEL and preKar2

Expression

Yeast

Available since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pICSL11059

Plasmid

#68263

Purpose

Level 1 Golden Gate Cassette: Plant hygromycin (with intron) resistance cassette

Depositor

Nicola Patron

Publication

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter:35s + 5'UTR:Omega + CDS:hptIIwintron + 3'UTR/terminator:35s

Use

Synthetic Biology

Expression

Plant

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pcDNA3 P-cad

Plasmid

#47502

Purpose

Expresses P-cadherin in mammalian cells

Depositor

Alice Wong

Publication

Cheung et al Oncogene. 2010 Apr 22;29(16):2427-40. do

Insert

cadherin 3, type 1, P-cadherin (placental) (CDH3 Human)

Expression

Mammalian

Promoter

CMV

Available since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pcDNA3-3myc-6His-EZH2 21A

Plasmid

#42663

Depositor

Mien-Chie Hung

Publication

Cha et al Science. 2005 Oct 14;310(5746):306-10.

Insert

EZH2 (EZH2 Human)

Tags

3Myc and 6His

Expression

Mammalian

Mutation

S21A

Available since

July 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pMK782 Septin6-GFP

Plasmid

#38296

Depositor

Matthew Krummel

Publication

Gilden et al J Cell Biol. 2012 Jan 9;196(1):103-14.

Insert

Septin 6 (Septin6 Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

22 amino acid linker sequence (added by PCR)

Promoter

CMV

Available since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3.1-(empty)-TAG

Plasmid

#138209

Purpose

To facilitate restriction cloning of an RNA of interest with a TGT recognition element in the 3' region

Depositor

Neal Devaraj

Publication

Busby et al Methods Enzymol. 2020;641:373-399. doi:

Type

Empty backbone

Use

T7 transcription

Tags

TAG sequence

Expression

Mammalian

Promoter

CMV Promoter, T7 Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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2Flag-pDEST-C

Plasmid

#118372

Purpose

Gateway compatible mammalian expression vector with C-terminal 2Flag to tag proteins of interest.

Depositor

Maria Vartiainen

Publication

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

2Flag

Expression

Mammalian

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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EB3-mScarlet-I

Plasmid

#98826

Purpose

In vivo visualization of microtuble plus ends (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

EB3

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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R-pre-GFP

Plasmid

#17274

Depositor

Sergio Grinstein

Publication

Yeung et al Science. 2006 Jul 21. 313(5785):347-51.

Insert

R-pre

Tags

EGFP

Expression

Mammalian

Available since

April 2, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles
PX852

Plasmid

#62884

Purpose

C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).

Depositor

Feng Zhang

Publication

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa536-1368)

Use

CRISPR

Tags

FKBP12, NLS SV40, and NLS nucleoplasmin

Expression

Mammalian

Promoter

CBh

Available since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBEVY-GL

Plasmid

#51225

Purpose

bi-directional expression vector for yeast, galactose inducible, LEU2 selection

Depositor

Charles Miller

Publication

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Expression

Yeast

Promoter

GAL1,10

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pUC8 human IFN-gamma

Plasmid

#17600

Depositor

Howard Young

Publication

Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

Insert

interferon-gamma cDNA (IFNG Human)

Expression

Bacterial

Available since

May 11, 2012

Availability

Academic Institutions and Nonprofits only
pFUGW mCherry-KASH

Plasmid

#131505

Purpose

mCherry_KASH epression under hUBC promotor; serves as backbone for ORANGE lenti knock-in

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

mCherry_KASH

Use

Lentiviral

Expression

Mammalian

Promoter

hUBC

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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7b sgRNA for EJ7-GFP reporter

Plasmid

#113624

Purpose

sgRNA/CAS9 expression plasmid to induce the 3’ double-strand break in the EJ7-GFP reporter

Depositor

Jeremy Stark

Publication

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7b sgRNA

Use

CRISPR

Expression

Mammalian

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles
pLV_Zika_NS4B_Flag

Plasmid

#79640

Purpose

Third generation Self-inactivating lentivector expressing NS4B from Zika virus

Depositor

Vaithi Arumugaswami

Insert

NS4B

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF1

Available since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Brachyury-eGFP

Plasmid

#21063

Depositor

Mark Mercola

Publication

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

Brachyury promoter

Use

Lentiviral

Tags

eGFP

Available since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRVGP

Plasmid

#15377

Depositor

Stephen Smale

Publication

Ramirez-Carrozzi et al Genes Dev. 2006 Feb 1. 20(3):282-96.

Insert

d2EGFP

Use

Retroviral

Expression

Mammalian

Available since

April 18, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCMV5 HA FoxO1

Plasmid

#14936

Depositor

Joan Massague

Publication

Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

Insert

FoxO1 (Foxo1 Mouse)

Tags

HA

Expression

Mammalian

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
1479 pcDNA3 flag HA 14-3-3 gamma

Plasmid

#9000

Depositor

William Sellers

Insert

14-3-3 gamma (Ywhag Rat)

Tags

HA and flag

Expression

Mammalian

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article