We narrowed to 16,261 results for: igh

1. pDSP15 (5’Δ HygR + loxP + MCS + 5’Δ mScarlet-I)

   Plasmid

   #193853

   Purpose

   Promoterless insertion vector for split HygR/mScarlet-I landing pad

   Depositor

   Patrick Phillips

   Article

   Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

   Insert

   5' rps-0::HygR + 5' mScarlet-I
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   rps-0p

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGWB420

   Plasmid

   #74814

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-CMV-TetO2_mVenus

   Plasmid

   #113893

   Purpose

   Mammalian (inducible) expression of cytosolic mVenus

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Insert

   monomeric Venus
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV-MIE-TetO2

   Available since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGWB410

   Plasmid

   #74804

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with FLAG (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-N1 SENP6

   Plasmid

   #14583

   Depositor

   Mary Dasso

   Article

   Mukhopadhyay et al J Cell Biol. 2006 Sep 25. 174(7):939-49.

   Insert

   SENP6 (SENP6 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pGWB520

   Plasmid

   #74862

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAGM35171

   Plasmid

   #153222

   Purpose

   Level 1 position 1 part containing the Nos promoter, the BAR gene, and the Ocs terminator

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Insert

   Nos promoter, BAR gene, Ocs terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV_FLAG-NFL(WT)

   Plasmid

   #182659

   Purpose

   Expresses wild-type mouse neurofilament light chain with an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Insert

   wild type mouse neurofilament light chain (Nefl Mouse)
   

   Use

   Tags

   FLAG tag (DYKDDDDK)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWB450

   Plasmid

   #74832

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ccas9LacZ

    Plasmid

    #113083

    Purpose

    Enhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.

    Depositor

    Tibor Vellai

    Article

    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYFAC-CH3

    Plasmid

    #168979

    Purpose

    AMA1 based expression in filamentous fungi pYFAC-riboB carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA

    Depositor

    Yit Heng Chooi

    Article

    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

    Type

    Empty backbone

    Use

    Synthetic Biology; Heterologous expression in asp…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. mEos2-FilaminA-N-9

    Plasmid

    #57379

    Purpose

    Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    FilaminA (FLNA Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    D2626H

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. p-mCherry-C1-PsACR1

    Plasmid

    #85465

    Purpose

    Natural anion conducting Channelrhodopsin with red shifted absorption. Closing kinetics 80ms. Codon optimized for mammalian expression (human/mouse)

    Depositor

    Peter Hegemann

    Article

    Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.

    Insert

    Synthetic construct PsChR1 gene
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (+enhancer)

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMMK ENTR 2

    Plasmid

    #206261

    Purpose

    ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Type

    Empty backbone

    Use

    Multimate/gateway entr 2

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSFDuet-1_VNp-LZ-mNeongreen

    Plasmid

    #182392

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper-mNeongreen fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-LZ-mNeongreen
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDest-521

    Plasmid

    #159688

    Purpose

    Gateway destination vector for E. coli protein expression

    Depositor

    Dominic Esposito

    Article

    Tran et al Nat Commun. 2021 Feb 19;12(1):1176. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a_Pro-IL-36γ

    Plasmid

    #214345

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL36G (IL36G Human)
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET41a iGluSnFR

    Plasmid

    #119828

    Purpose

    Production of recombinant iGluSnFR, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. CVS-N2c-nl.mCherry-FlpO

    Plasmid

    #172378

    Purpose

    Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    nuclear-localized mCherry + FlpO
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPBT-peRNA_GG-Puro

    Plasmid

    #173220

    Purpose

    piggyBac transposon containing hU6 promotor and pegRNA cloning site

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

    Inserts

    pegRNA cloning cassette
    PuroR
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a and hU6

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pHH0103_YWHAZ_14-3-3

    Plasmid

    #109808

    Purpose

    Protein expression and purification of YWHAZ_14-3-3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    YWHAZ_14-3-3 (YWHAZ Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHR-PGK-LexA-WW-CIB1-biLINuS

    Plasmid

    #171757

    Purpose

    Lentiviral vector for constitutive expression of fusion protein LexA-WW-CIB1-biLINuS

    Depositor

    Yingxiao Wang

    Article

    Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

    Insert

    Synthetic gene containing LexA-WW-CIB1-biLINuS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUC57-Amp_CRISPIE donor B1

    Plasmid

    #172842

    Purpose

    CRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    CRISPIE designer exon (phase 0-0) encoding mEGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28a-ec_bPAC

    Plasmid

    #28135

    Purpose

    Expression of photoactivated cyclase bPAC of Beggiatoa sp. optimized for E. coli

    Depositor

    Peter Hegemann

    Article

    Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

    Insert

    E.coli adapted photoactivated adenylyl cyclase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCS2+PIF6CreN

    Plasmid

    #131780

    Purpose

    Mammalian, Zebrafish, or Xenopus expression of PIF6CreN, one half of the CreLite system

    Depositor

    George Eisenhoffer

    Article

    Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

    Insert

    PIF6CreN
    

    Use

    Cre/Lox; Zebrafish expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDL002_Hec1-ms_IgG_LC

    Plasmid

    #193619

    Purpose

    Hec1 light chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL001 in HEK293 cells.

    Depositor

    Jennifer DeLuca

    Article

    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

    Insert

    Anti-Hec1 light chain (Hec1-ms_IgG_LC)
    

    Use

    Tags

    signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pGWB508

    Plasmid

    #74850

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Oct. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDGE670

    Plasmid

    #153236

    Purpose

    Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHH0103_PDCD6_EF-hand

    Plasmid

    #110009

    Purpose

    Protein expression and purification of PDCD6_EF-hand

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    PDCD6_EF-hand (PDCD6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGWB418

    Plasmid

    #74812

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with 4xMyc (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge