We narrowed to 7,549 results for: URE

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Syntaxin1A(E49TAG)-mCherry

Plasmid

#241291

Purpose

Mammalian expression of syntaxin-1(E49TAG) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

E49TAG

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8∆60-C.a.URA3

Plasmid

#240970

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; C.a.URA3 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8d∆60-TRP1

Plasmid

#240968

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; TRP1 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8∆60-K.I.LEU2

Plasmid

#240969

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; K.l.LEU2 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8∆60-BleMX6

Plasmid

#240974

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; BleMX6 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

BleMX6

Expression

Bacterial

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8∆60-His3MX6

Plasmid

#240971

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; HIS3 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+her3WT-P2A-sfGFP

Plasmid

#240545

Purpose

Wild-type zebrafish Her3 (ortholog to human HES3) in a -2A-sfGFP backbone for visualization

Depositor

Genevieve Kendall

Article

Kent et al Dev Biol. 2023 Apr;496:1-14. doi: 10.101

Insert

her3 (her3 Zebrafish)

Expression

Mammalian

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pUltra-EGFP-P2A-Tr-NRL

Plasmid

#239100

Purpose

pUltra-EGFP bi-cistronic lentiviral vector expressing EGFP and truncated NRL.

Depositor

David Cobrinik

Article

Shayler et al eLife 13:RP101918

Insert

Neural Retina Leucine Zipper (truncated) (NRL Human)

Use

Lentiviral

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pZNp-2

Plasmid

#245373

Purpose

Negative control, firefly luciferase mutated

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-wt (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pZ_pF-3

Plasmid

#245374

Purpose

Negative control, NanoLuciferase mutated

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-wt (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pZNpF-wt

Plasmid

#245364

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-wt (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4-C-EF1α-Tr-NRL

Plasmid

#239096

Purpose

Expresses truncated NRL, driven by EF1α promoter.

Depositor

David Cobrinik

Article

Shayler et al eLife 13:RP101918

Insert

Neural Retina Leucine Zipper (truncated) (NRL Human)

Expression

Mammalian

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pACE-MBP-FBXL17 (310–701)

Plasmid

#228369

Purpose

express human FBXL17 in insect cells, such as Trichoplusia ni Hi5

Depositor

Ning Zheng

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

FBXL17 (FBXL17 Human)

Tags

MBP

Expression

Insect

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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MZB131

Plasmid

#217839

Purpose

Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged AP3D1 adaptin appendage (920-1153) for pulldown assays

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

AP3D1 (AP3D1 Human)

Tags

10x His, HaloTag, and SUMO (SMT3)

Expression

Bacterial

Mutation

Deleted residues 1-919

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-PGK-TetOn-Puro_MeltITSN1-37-mch

Plasmid

#234068

Purpose

Encodes the Dbl homology and pleckstrin homology (DH-PH) domain of Intersectin1 controlled by a Melt variant with a switching temperature of 37°C

Depositor

Lukasz Bugaj

Article

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

MeltITSN1-37-mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAM59

Plasmid

#227655

Purpose

pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, Δ78–81)

Depositor

Andreas Martin

Article

Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-

Inserts

Rpn11 (RPN11 Budding Yeast)
Rpn8 (RPN8 Budding Yeast)

Tags

H6-PreScission and PreScission-Strep

Expression

Bacterial

Mutation

1-179 and amino acids 2-239, Δ78–81

Promoter

T7

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM54

Plasmid

#227653

Purpose

pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, L132A)

Depositor

Andreas Martin

Article

Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-

Inserts

Rpn11 (RPN11 Budding Yeast)
Rpn8 (RPN8 Budding Yeast)

Tags

H6-PreScission and PreScission-Strep

Expression

Bacterial

Mutation

1-179 and amino acids 2-239, L132A

Promoter

T7

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL.mEGFP-CBX8

Plasmid

#224710

Purpose

Expresses N-terminal hexahistidine-tagged human CBX8 in insect cells, with a monomeric variant EGFP tag.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 (CBX8 Human)

Tags

mEGFP

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pET28-a(+)-6xHis-miRFP(-5)-HO1

Plasmid

#219424

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Inserts

miRFP682
HO1

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

Contains Ribosomal Binding Site for expression of…

Promoter

T7

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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MAC3_C_VPS35L(A830T)

Plasmid

#209693

Purpose

Expresses MAC3-tagged VPS35L(A830T) in mammalian cells

Depositor

Markku Varjosalo

Article

Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

Insert

VPS35L (VPS35L Human)

Tags

MAC3-tag

Expression

Mammalian

Mutation

A830T, changed Alanine 830 to Threonine

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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