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Prham-M.PstI-p15A-aadA

Plasmid

#149340

Purpose

expresses M.PstI DNA methyltransferase in E. coli

Depositor

Ichiro Matsumura

Article

Matsumura et al PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717

Insert

M.,PstI

Expression

Bacterial

Promoter

rhamnose

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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Prham-M.XbaI-p15A-aadA

Plasmid

#149338

Purpose

expresses M.XbaI DNA methyltransferase in E. coli

Depositor

Ichiro Matsumura

Article

Matsumura et al PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717

Insert

M.XbaI

Expression

Bacterial

Promoter

rhamnose

Available Since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046613

Plasmid

#149200

Purpose

Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pTEF1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046481

Plasmid

#149093

Purpose

Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

wt

Promoter

pTEF1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046265

Plasmid

#149045

Purpose

Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pTEF1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046487

Plasmid

#149027

Purpose

Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;MBP

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046522

Plasmid

#145530

Purpose

Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;StrepII

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046364

Plasmid

#145491

Purpose

Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046214

Plasmid

#145399

Purpose

Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;StrepII

Expression

Yeast

Mutation

wt

Promoter

pTEF1

Available Since

April 28, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pJK593

Plasmid

#71587

Purpose

Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag (H6YfdE)

Depositor

T. J. Kappock

Article

Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

Insert

acetyl-CoA:oxalate CoA-transferase (yfdE )

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pJK594

Plasmid

#71588

Purpose

Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with C-terminal His6 tag (YfdEH6)

Depositor

T. J. Kappock

Article

Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

Insert

acetyl-CoA:oxalate CoA-transferase (yfdE )

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pWZ414-F15

Plasmid

#23064

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H4 deleted amino acids 4 - 19

Available Since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F23

Plasmid

#23065

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H4 changed Lysine 5 to Glutamine

Available Since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F33

Plasmid

#23068

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H4 changed Lysine 5 to Glutamine H4 K5Q

Available Since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F47

Plasmid

#23071

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H4 changed Lysines 8 and 16 to Glutamines…

Available Since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F49

Plasmid

#23073

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H4 changed Lysines 8 and 16 to Arginine, …

Available Since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F51

Plasmid

#23075

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H4 changed Lysines 5 and 12 to Glutamine,…

Available Since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F52

Plasmid

#23076

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H4 changed Lysines 5 and 12 to Arginines,…

Available Since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F36

Plasmid

#23069

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 14 to Glycine, H3 K14G

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F43

Plasmid

#23070

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 14 to Arginine, H3 K14R

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only