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CAG-mRuby2(miRE.FF4)-TS.FF6x1-bGH

Plasmid

#235297

Purpose

ComMAND open-loop circuit regulating mRuby2, CAG-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG (synthetic cytomegalovirus early enhancer + c…

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miRE.FF4)-TS.FF4x4-bGH

Plasmid

#235295

Purpose

ComMAND closed-loop circuit regulating mRuby2 with 4 copies of microRNA target sites

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pSE89: pFuGW-G5p-EYFP

Plasmid

#240461

Purpose

Synthetic GAL4p comprising 5 tandem GAL4 binding sites (G5p) regulating EYFP

Depositor

Lior Nissim

Article

Edri et al Cell Rep Methods. 2025 Aug 5:101139. doi

Inserts

G5p
EYFP

Use

Lentiviral

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pSE92: pFuGW-G14p-EYFP

Plasmid

#240462

Purpose

A synthetic GAL4 promoter comprising 14 tandem GAL4 binding sites (G14p) regulating EYFP

Depositor

Lior Nissim

Article

Edri et al Cell Rep Methods. 2025 Aug 5:101139. doi

Inserts

EYFP
G14p

Use

Lentiviral

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pJV170

Plasmid

#180077

Purpose

Backbone to clone MTase genes for expression in TXTL

Depositor

Chase Beisel

Article

Vento et al Mol Cell. 2024 Jul 25;84(14):2785-2796.e

Type

Empty backbone

Expression

Bacterial

Promoter

P70a

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pPBpuro-chGrb2/eDHFR(69K6)-iRFP713

Plasmid

#214828

Purpose

Encoding Grb2-eDHFR(69K6) chimera fused to iRFP713

Depositor

Michiyuki Matsuda

Article

Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

Insert

Grb2(1-59)-eDHFR(69K6)-Grb2(152-216)-iRFP713

Tags

iRFP713

Expression

Mammalian

Promoter

CAG promoter

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-TS.FF5x1-mRuby2-miRE.FF5-bGH

Plasmid

#235259

Purpose

ComMAND closed-loop circuit regulating mRuby2 with miRE-FF5 design 3

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-TS.FF6x1-mRuby2-miRE.FF5-bGH

Plasmid

#235258

Purpose

ComMAND open-loop circuit regulating mRuby2 with miRE-FF5 design 3

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-TS.FF6x1-mRuby2(miR.FF4)-bGH

Plasmid

#235284

Purpose

ComMAND open-loop circuit regulating mRuby2 with miR-FF4 design 2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miRE.FF4)-TS.FF6x4-bGH

Plasmid

#235293

Purpose

ComMAND open-loop circuit regulating mRuby2 with 4 copies of microRNA target sites

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-TS.FF6x1-mRuby2(miR.FF5)-bGH

Plasmid

#235278

Purpose

ComMAND open-loop circuit regulating mRuby2 with miR-FF5 design 2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-TS.FF5x1-mRuby2-miR.FF5-bGH

Plasmid

#235281

Purpose

ComMAND closed-loop circuit regulating mRuby2 with miR-FF5 design 3

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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EFS-mRuby2(miRE.FF4)-TS.FF6x1-bGH

Plasmid

#235309

Purpose

ComMAND open-loop circuit regulating mRuby2, EFS-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EFS (human EF1a short, intronless)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-TS.FF6x1-mRuby2-miR.FF5-bGH

Plasmid

#235280

Purpose

ComMAND open-loop circuit regulating mRuby2 with miR-FF5 design 3

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-TS.FF5x1-mRuby2(miR.FF5)-bGH

Plasmid

#235279

Purpose

ComMAND closed-loop circuit regulating mRuby2 with miR-FF5 design 2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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hPGK-mRuby2(miRE.FF4)-TS.FF6x1-bGH

Plasmid

#235311

Purpose

ComMAND open-loop circuit regulating mRuby2, hPGK-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

hPGK (human phosphoglycerate kinase)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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hPGK-mRuby2(miRE.FF4)-TS.FF4x1-bGH

Plasmid

#235312

Purpose

ComMAND closed-loop circuit regulating mRuby2, hPGK-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

hPGK (human phosphoglycerate kinase)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG-mRuby2(miRE.FF4)-TS.FF4x1-bGH

Plasmid

#235298

Purpose

ComMAND closed-loop circuit regulating mRuby2, CAG-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG (synthetic cytomegalovirus early enhancer + c…

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-[EF1a-mRuby2-bGH]-UbC-PuroR-T2A-mGL-SV40pA

Plasmid

#235325

Purpose

PiggyBac integration vector for ComMAND base gene regulating mRuby2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR221-Illusia(YYEE)

Plasmid

#222591

Purpose

Gateway entry vector with a phospho-mimetic mutant (YYEE) of the Illusia FRET reporter for integrin phosphorylation

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Illusia(YYEE)

Use

Gateway entry vector

Mutation

Mutations in the NPxY sites equivolent to Y783E, …

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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