We narrowed to 7,201 results for: mme

1. pAAV-CAG-EGFP-P2A-FusionRed

   Plasmid

   #191108

   Purpose

   Mammalian cell line expression plasmids for production of EGFP and FusionRed proteins

   Depositor

   Kiryl Piatkevich

   Article

   Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

   Insert

   pAAV-CAG-EGFP-P2A-FusionRed
   

   Use

   AAV

   Tags

   FusionRed

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   chicken β-actin promoter

   Available Since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-Flag-Kdm6b(1025-End)_H1390A-pA

   Plasmid

   #100279

   Purpose

   In vitro transcription template (T7 promoter) for Kdm6b catalytic domain mutant

   Depositor

   Yi Zhang

   Article

   Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

   Insert

   Kdm6b (Kdm6b Mouse)
   

   Tags

   Flag epitope tag

   Expression

   Mammalian

   Mutation

   Mutated catalytic domain (1025aa-End) (please see…

   Available Since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti_CamKIIa_mKate2

   Plasmid

   #96942

   Purpose

   To label mature excitatory iPSC-derived human neurons

   Depositor

   Oleksandr Shcheglovitov, Ricardo Dolmetsch

   Article

   Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

   Insert

   far-red fluorescent protein
   

   Use

   Lentiviral

   Mutation

   E87D (please see depositor comments below)

   Promoter

   CaMKIIa

   Available Since

   Aug. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTrc99S-ssDsbA-MBP-4xDQNAT

   Plasmid

   #128398

   Purpose

   Periplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Maltose binding protein
   

   Tags

   4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

   Expression

   Bacterial

   Mutation

   Y342H- please see depositor comments

   Promoter

   trc

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET29a-hNABP2

   Plasmid

   #128307

   Purpose

   Express hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tag

   Depositor

   Yuliang Wu

   Article

   Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

   Insert

   Human Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Y85C- please see depositor comments below

   Promoter

   T7

   Available Since

   Aug. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFUW-tetO-NR4A3

   Plasmid

   #139818

   Purpose

   doxycycline-inducible expression of Human NR4A3 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   NR4A3 (NR4A3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   S240G- please see depositor comment below

   Promoter

   TRE-mCMV

   Available Since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBTK569

   Plasmid

   #110614

   Purpose

   BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by CP25 promoter

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   E2-Crimson
   

   Expression

   Bacterial

   Mutation

   V32M (see comments below)

   Available Since

   Oct. 2, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pEMI91

   Plasmid

   #74888

   Purpose

   Shuffled 9I27 polyprotein with shuffled codons to allow facile sequencing and unique RE for the 9 cassettes for versatile cloning. See Depositor Comments for more information

   Depositor

   Piotr Marszalek

   Article

   Scholl et al Biomacromolecules. 2016 Jun 8.

   Insert

   Titin I27 x 9 (TTN Human)
   

   Tags

   His-tag and Strep-tag II

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV6M-Pak1 H83L H86L

   Plasmid

   #12211

   Depositor

   Jonathan Chernoff

   Article

   Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

   Insert

   Pak1 (PAK1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   PBD (p21-binding domain) mutant: H83L, H86L (see …

   Available Since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRB117.2

    Plasmid

    #181949

    Purpose

    aTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    mNG-NarL; mCherry; tetR (narL, AX04_RS23485 narL - E. coli, Synthetic)
    

    Tags

    mNeonGreen

    Expression

    Bacterial

    Mutation

    See Depositor Comments Below

    Promoter

    mNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7-EGFP-C1-HsNXF-1-5xmut_O

    Plasmid

    #147116

    Purpose

    Mammalian Expression of HsNxf-1-5xmut

    Depositor

    Elisa Izaurralde

    Article

    Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

    Insert

    HsNxf-1-5xmut (NXF1 Human)
    

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBMN mCherry-TAX1BP1(V142S/ΔUBD)

    Plasmid

    #119690

    Purpose

    For mammalian expression of LIR mutant TAX1BP1 without its UBD

    Depositor

    Michael Lazarou

    Article

    Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

    Insert

    TAX1BP1 (TAX1BP1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    V142S & UBD deletion (1-755), See depositor c…

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. VVT1

    Plasmid

    #65779

    Purpose

    The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    SaCas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF6 VPS51-V5-6His

    Plasmid

    #175502

    Purpose

    V5 tagged VPS51 is expressed in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Perez-Victoria et al Mol Biol Cell. 2010 Oct 1;21(19):3386-95

    Insert

    VPS51 (VPS51 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    A661V- please see depositor comments

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLJC6-GPT1-3xFLAG

    Plasmid

    #163448

    Purpose

    lentiviral overexpression vector for human GPT1

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    GPT1 (GPT Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    See: comments

    Promoter

    Ubc

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. SEP-Nanoluc-cyOFP1

    Plasmid

    #141082

    Purpose

    prototype of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    SEP-Nanoluc-cyOFP1
    

    Use

    Lentiviral

    Mutation

    Deletion of amino acids DISGG from positions 673 …

    Promoter

    EF1α

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. DJ-1 TEV site pET15b

    Plasmid

    #60687

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    DJ-1 (PARK7 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    TEV site was inserted (see comments below).

    Promoter

    T7

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

18. Sumo3 PolQM1 deltains2

    Plasmid

    #78460

    Purpose

    Inducible expression in E. coli

    Depositor

    Susan Wallace, Sylvie Doublie

    Article

    Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

    Insert

    Polymerase theta (POLQ Human)
    

    Tags

    His and SUMO3

    Expression

    Bacterial

    Mutation

    delta 1-1791; delta 2264-2315; Q2513R (see deposi…

    Available Since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pGL3-24p3/LCN2-282kB mut-LUC

    Plasmid

    #46867

    Purpose

    murine 24p3 minimal promoter with NF-kB mutation as a luciferase reporter

    Depositor

    Sarah Gaffen

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    24p3/lipocalin 2 proximal promoter
    

    Use

    Luciferase

    Mutation

    NF- kB site is mutated (see comments)

    Promoter

    24p3 promoter (inactive)

    Available Since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pGL3-24p3/LCN2-282C/EBP mut-LUC

    Plasmid

    #46868

    Purpose

    murine 24p3 minimal promoter with C/EBP mutation k as a luciferase reporter

    Depositor

    Sarah Gaffen

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    24p3/lipocalin 2 proximal promoter
    

    Use

    Luciferase

    Mutation

    CEBP site is mutated (see comments)

    Promoter

    24p3 promoter (inactive)

    Available Since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only