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Showing: 3151 - 3160 of 164800 results

  1. pmCherry-3xNLS-OptoJNKi-dsm

    Plasmid
    #89743
    Purpose
    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11
    Depositor
    Michael Courtney
    Article
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Insert
    NLS-OptoJNKi dark-state mutant
    Use
    Fluorescent protein
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    C450A mutation of AsLov2Jalpha
    Promoter
    CMV
    Available Since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    89743-plasmid-map-sequence-id-192503

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  2. pmCherry-3xNLS-OptoJNKi-lsm

    Plasmid
    #89742
    Purpose
    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11
    Depositor
    Michael Courtney
    Article
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Insert
    NLS-OptoJNKi lit-state mutant
    Use
    Fluorescent protein
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    I539E mutation of AsLov2Jalpha
    Promoter
    CMV
    Available Since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    89742-plasmid-map-sequence-id-192502

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  3. pGTM_SUMO_PLpro_C111S

    Plasmid
    #233740
    Purpose
    Expresses SARS-CoV-2 Nsp3 residues 746-1063 for the purification of PLpro protease with a C111S active site mutation that abolishes protease activity. Following Sumo Protease cleavage no nonnative res
    Depositor
    Gaetano Montelione
    Article
    De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do
    Insert
    PLpro (ORF1ab SARS-CoV-2)
    Tags
    6xHis-Sumo
    Expression
    Bacterial
    Mutation
    Cys111Ser active site mutation of the Papain-Likeā€¦
    Promoter
    T7 lac
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    233740-plasmid-map-sequence-id-469323

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  4. pGTM_Sumo2-N-8XHis-Plpro

    Plasmid
    #233803
    Purpose
    Expresses SARS-CoV-2 Nsp3 residues 746-1063 for the purification of an active PLpro protease. Following Sumo Protease Cleavage an 8xHis Tag is retained at the N-terminus
    Depositor
    Gaetano Montelione
    Article
    De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do
    Insert
    orf1ab (ORF1ab SARS-CoV-2)
    Tags
    6xHis-Sumo and 8xHis
    Expression
    Bacterial
    Promoter
    T7 lac
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    233803-plasmid-map-sequence-id-469494

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  5. pGTM_Sumo2-C-8XHis-PLpro

    Plasmid
    #234321
    Purpose
    Expresses SARS-CoV-2 Nsp3 residues 746-1063 for the purification of an active PLpro protease. Following Sumo Protease Cleavage an 8xHis Tag is retained at the C-terminus
    Depositor
    Gaetano Montelione
    Article
    De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do
    Insert
    PLpro (ORF1ab SARS-CoV-2)
    Tags
    6xHis-Sumo and 8xHis
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7 lac
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    234321-plasmid-map-sequence-id-469248

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  6. pGTM_Sumo2-N-StrepII-PLpro

    Plasmid
    #234322
    Purpose
    Expresses SARS-CoV-2 Nsp3 residues 746-1063 for the purification of an active PLpro protease. Following Sumo Protease Cleavage a Strep-tag II sequence is retained at the N-terminus
    Depositor
    Gaetano Montelione
    Article
    De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do
    Insert
    PLpro (ORF1ab SARS-CoV-2)
    Tags
    6xHis-Sumo and Strep-tag II
    Expression
    Bacterial
    Promoter
    T7 lac
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    234322-plasmid-map-sequence-id-469910

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  7. pGTM_Sumo2-N-8XHis-Plpro-C111S

    Plasmid
    #234472
    Purpose
    Expresses SARS-CoV-2 Nsp3 residues 746-1063 C111S with an activity abolishing mutation for the purification of this PLpro protease. Following Sumo Protease Cleavage an N-term 8xHis Tag is retained
    Depositor
    Gaetano Montelione
    Article
    De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do
    Insert
    PLpro (ORF1ab SARS-CoV-2)
    Tags
    6xHis-Sumo and 8xHis
    Expression
    Bacterial
    Promoter
    T7 lac
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    234472-plasmid-map-sequence-id-469247

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  8. pGTM_Sumo_PLpro_C111A

    Plasmid
    #234489
    Purpose
    Expresses SARS-CoV-2 Nsp3 residues 746-1063 for the purification of PLpro protease with a C111A active site mutation that abolishes protease activity. Following Sumo Protease cleavage no nonnative res
    Depositor
    Gaetano Montelione
    Article
    De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do
    Insert
    PLpro (ORF1ab SARS-CoV-2)
    Tags
    6xHis-Sumo
    Expression
    Bacterial
    Promoter
    T7 lac
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    234489-plasmid-map-sequence-id-469250

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Showing: 3151 - 3160 of 164800 results