We narrowed to 11,247 results for: SHA

1. pFE-Selo-FI-prk

   Plasmid

   #162698

   Purpose

   Expresses S. elongatus Form IB rubisco and S. elongatus Prk

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   S. elongatus Form IB rubisco and S. elongatus Prk
   

   Use

   Tags

   N terminal 6x His tag on PRK

   Expression

   Bacterial

   Mutation

   Promoter

   PLtet0-1 promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. NSUN2

   Plasmid

   #155793

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   NSUN2 (NSUN2 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. VEGF binding scFv G6

   Plasmid

   #111716

   Purpose

   Yeast Surface Display of G6 as a reference starting point for design

   Depositor

   Sarel Fleishman

   Article

   Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

   Insert

   G6
   

   Use

   Tags

   cmyc tag

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV V5-hRnf185

   Plasmid

   #175127

   Purpose

   Lentiviral expression of V5-tagged human RNF185

   Depositor

   Larry Gerace

   Article

   Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

   Insert

   RNF185 (RNF185 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. TIA1 RRM-mCherry-iLIDx6

   Plasmid

   #103782

   Purpose

   expresses iPOLYMER component in mammalian cells

   Depositor

   Takanari Inoue

   Article

   Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

   Insert

   TIA1 RRM-mCherry-iLIDx6
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-kanMX6-P41nmt-3HA

   Plasmid

   #39284

   Depositor

   Jurg Bahler, John Pringle

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   nmt1 promoter (nmt1 Fission Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   3xHA, A. gossypii translation elongation factor 1…

   Expression

   Mutation

   -1163 to +6 of nmt1 locus with a 4bp deletion (TA…

   Promoter

   Available since

   Aug. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pFA6a-kanMX6-P81nmt1-3HA

   Plasmid

   #39285

   Depositor

   Jurg Bahler, John Pringle

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   nmt1 promoter (nmt1 Fission Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   3xHA, A. gossypii translation elongation factor 1…

   Expression

   Mutation

   -1163 to +6 of nmt1 locus with a 7bp deletion (AT…

   Promoter

   Available since

   Aug. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pGZ109 (pFA6a-3FLAG-MNase-HIS3MX6)

   Plasmid

   #70232

   Purpose

   ChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in pFA6a-3HA- HIS3MX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope

   Depositor

   Steven Henikoff

   Article

   Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

   Insert

   3xFLAG tag and aa 83-231 of Mnase
   

   Use

   Yeast genomic targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. E. coli expression: Z-lock cofilin dark state mutant

   Plasmid

   #141135

   Purpose

   a control for optogenetic cofilin analog

   Depositor

   Klaus Hahn

   Article

   Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

   Insert

   Zdk2, Cofilin, LOV2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D3 deletion, I32F in Zdk2

   Promoter

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBLO 62.4

    Plasmid

    #123123

    Purpose

    Human expression vector for deltaproteobacteria CasX and sgRNA

    Depositor

    Jennifer Doudna, Benjamin Oakes

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Inserts

    CasX protein
    CasX sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCS2-hDKK1-flag

    Plasmid

    #15494

    Depositor

    Sergei Sokol

    Article

    Krupnik et al Gene. 1999 Oct 1. 238(2):301-13.

    Insert

    DKK1 (DKK1 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2007

    Availability

    Academic Institutions and Nonprofits only

12. SR140

    Plasmid

    #156037

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SR140 (U2SURP Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. sgTrack-BFP

    Plasmid

    #164273

    Purpose

    Empty sgRNA expression vector with co-expression of BFP reporter

    Depositor

    Charles Rudin

    Article

    Wohlhieter et al Cell Rep. 2020 Dec 1;33(9):108444. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EFS

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-PITCh-MYO1C

    Plasmid

    #227305

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of MYO1C for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of MYO1C (MYO1C Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-ITR-PytR-mCherry

    Plasmid

    #204868

    Purpose

    Expresses pyrrolysyl tRNA and a wild-type mCherry reporter; can be packaged into AAV

    Depositor

    Abhishek Chatterjee

    Article

    Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

    Insert

    M. mazei pyrrolysyl tRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    U25C

    Promoter

    U6

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFE-Rrub-FII-prk

    Plasmid

    #162695

    Purpose

    Expresses R. rubrum Form II rubisco and S. elongatus Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Inserts

    Rubrum Form II rubisco
    S. elongatus PRK
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter and under same promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBLO 62.5

    Plasmid

    #123124

    Purpose

    Human expression vector for planctomyces CasX and sgRNA

    Depositor

    Jennifer Doudna, Benjamin Oakes

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Inserts

    CasX protein
    CasX sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-cofilin-S3D

    Plasmid

    #78297

    Purpose

    Mammalian expression of mouse cofilin S3D fused to EGFP

    Depositor

    Michael Frotscher

    Article

    Chai et al Development. 2016 Mar 15;143(6):1029-40.

    Insert

    cofilin 1 (Cfl1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S3D

    Promoter

    CAG

    Available since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE2 GFP-Atl1

    Plasmid

    #86679

    Purpose

    Lentivirus to stably express fluorescent protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    ATL1 (ATL1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEJS887_pTetR-P2A-BFPnls/sgAlpha

    Plasmid

    #108651

    Purpose

    Alpha satellite repeats targeting Spy sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Inserts

    Alpha satellite repeats targeting sgRNA
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hPGK

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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