We narrowed to 14,132 results for: flag

1. pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A-NLS-mut

   Plasmid

   #42045

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   TNRC6A (TNRC6A Human)
   

   Tags

   FLAG, HA, and SBP

   Expression

   Mammalian

   Mutation

   R930A, R931A, R932A, R934A

   Available Since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKT-mIDH1(R132H)-Myc-FLAG-IRES-Kat

   Plasmid

   #133779

   Purpose

   Expresses IDH1 with a R132H mutation and has Myc and FLAG tags. IDH1(R132H) also has a katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

   Depositor

   Maria Castro

   Insert

   Isocitrate dehydrogenase 1 (IDH1 Human)
   

   Tags

   Myc and FLAG

   Expression

   Mammalian

   Mutation

   Arginine is mutated to histidine at amino acid po…

   Available Since

   Oct. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. RRL.sin.cPPT.SFFV/Flag-NCOA7 variant 6.IRES-puro.WPRE (CG536)

   Plasmid

   #139443

   Purpose

   Lentiviral vector to ectopically express human NCOA7 isoform 4 from SFFV promoter

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   FLAG-tag NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti CMV-SLC7A11-sh926R-FLAG-IRES-Hygro

   Plasmid

   #118702

   Purpose

   lentiviral vector experessing SLC7A11 insensitive to hairpin TRCN0000288926

   Depositor

   William Kaelin

   Article

   Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

   Insert

   SLC7A11 (SLC7A11 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Mutation

   wt , SLC7A11 sh926R

   Available Since

   Dec. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Flag::UbvG08 P69L, L70V, I44A, deltaGG

   Plasmid

   #74940

   Purpose

   Ubiquitin variant with mutations L69P and V70L reverted to wild type (i53-DM mutant)

   Depositor

   Daniel Durocher

   Article

   Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

   Insert

   Ubiquitin
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   UbvG08 with I44A, P69L, and L70V mutations and no…

   Promoter

   CMV

   Available Since

   May 13, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pLenti HsATP10B_WT-T2A-His-flag-TMEM30A variant1

   Plasmid

   #191675

   Purpose

   transfer plasmid for lentiviral vector production expressing Hs ATP10B WT and His/Flag tagged Hs TMEM30A

   Depositor

   Veerle Baekelandt

   Inserts

   ATP10B (ATP10B Human)
   TMEM30A (TMEM30A Human)
   

   Use

   Lentiviral

   Tags

   6X his and flag

   Available Since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCBh_3x Flag-NLS-OsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

   Plasmid

   #197024

   Purpose

   Vector encoding a human codon-optimized OsCas12f1 driven by CBh promoter, guide RNAs compatible with OsCas12f1 driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Xiangfeng Kong

   Article

   Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

   Insert

   humanized OsCas12f1
   

   Expression

   Mammalian

   Promoter

   CBh, CMV, hU6

   Available Since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-2xFLAG-2xSTREP-CCND1(T286A)-IRES-mCherry

   Plasmid

   #172639

   Purpose

   Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286A) and mCherry in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Lentiviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Thr286Ala

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. p6348 MSCV-CMV-Flag-HA-Brd4 1047-1362

   Plasmid

   #31354

   Depositor

   Peter Howley

   Article

   Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

   Insert

   Brd4 1047-1362 (BRD4 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Available Since

   Sept. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-GFP

    Plasmid

    #90012

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-GFP

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    CBirA*-Cdc25C
    NBirA*-GFP
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. p6347 MSCV-CMV-Flag-HA-Brd4-444-722

    Plasmid

    #31353

    Depositor

    Peter Howley

    Article

    Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

    Insert

    Brd4 444-722 (BRD4 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Available Since

    Oct. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBIG2abc_nsp12-His6-3xFlag/nsp7/nsp8 (SARS-CoV-2)

    Plasmid

    #169185

    Purpose

    Baculoviral transfer vector to co-express nsp12-His6-3xFlag, nsp7 and nsp8 (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    nsp12-His6-3xFlag/nsp7/nsp8 (ORF1ab SARS-CoV-2, Synthetic)
    

    Tags

    His6-3xFlag (nsp12 C-terminus)

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcFLAG-CSK-delta SH3 (73 to450 amino acids)

    Plasmid

    #74504

    Purpose

    Overexpression of CSK delta SH3 in mammalian cells

    Depositor

    Lars Rönnstrand

    Article

    Kazi et al Cell Signal. 2013 Sep;25(9):1852-60. doi

    Insert

    CSK (CSK Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    del-SH3 (73 to450 amino acids)

    Promoter

    CMV

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pUC57-2xFLAG-mAID-AMBRA1 HDR donor template

    Plasmid

    #172609

    Purpose

    Donor plasmid containing the HDR template for the in-frame, N-terminal 2xFLAG-mAID tagging of AMBRA1 gene

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    2xFLAG-mAID-AMBRA1 (AMBRA1 Human, Synthetic)
    

    Use

    CRISPR; Human targeting

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. JGE301_pAAV-hSyn-DIO-Flag-hM3Dq-V2(2D)-ABIcs-mCherry

    Plasmid

    #107246

    Purpose

    hM3DREADD fused to ABIcs for abscisic acid mediated recruitment of PYLcs labelled proteins.

    Depositor

    Bryan Roth

    Article

    Roth et al bioRxiv 251769

    Insert

    hM3DREADD
    

    Use

    AAV

    Tags

    ABIcs, FLAG, and mCherry

    Mutation

    T to D and S to D mutations in V2-tail

    Promoter

    hSYN

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N-D231G

    Plasmid

    #113953

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    delete N-terminal 21 residues, I132S, S212N, and …

    Promoter

    cmv

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEF5/FRT-DEST EVC2-TEV-YFP-Flag

    Plasmid

    #41004

    Depositor

    Rajat Rohatgi

    Article

    Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

    Insert

    Ellis van Creveld syndrome 2 (EVC2 Mouse)
    

    Tags

    TEV-YFP-Flag

    Expression

    Mammalian

    Available Since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. p6349 MSCV-CMV-Flag-HA-Brd4 1224-1362

    Plasmid

    #31355

    Depositor

    Peter Howley

    Article

    Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

    Insert

    Brd4 1224-1362 (BRD4 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Available Since

    Sept. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTRIP-MND-HsMAN1C1-1-630-cMycFlag-hPGK-GFP

    Plasmid

    #183505

    Purpose

    Express GFP and MAN1C1 proteins

    Depositor

    Olivier Guipaud

    Article

    Ladaigue et al iScience. 2022 Nov 2;25(12):105482. doi:

    Inserts

    man1C1 (MAN1C1 Human)
    EGFP
    

    Use

    Lentiviral

    Tags

    Myc-DDK

    Expression

    Mammalian

    Promoter

    MND and hPGK

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSCV-Neo-GFP/V5-Foxa1-(P2A)-Flag-Gata5

    Plasmid

    #105509

    Purpose

    MSCV-driven retroviral Foxa1 and Gata5 expression (P2A-linked, V5 and Flag-tagged each)

    Depositor

    Christopher Vakoc

    Article

    Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

    Inserts

    Foxa1 (Foxa1 Mouse)
    Gata5 (Gata5 Mouse)
    

    Use

    Retroviral

    Tags

    Flag and V5

    Available Since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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