We narrowed to 10,337 results for: plasmids 101

1. pcDNA3-HCMVgL-FLAG (TB40/E)

   Plasmid

   #136449

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein L (TB40/E strain) with FLAG tag

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein L (UL115 Human betaherpesvirus 5)
   

   Use

   Tags

   Short SGGSG linker and FLAG tag

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Syn1-fDIO-mScarlet-KASH-2a-dCre

   Plasmid

   #203839

   Purpose

   Flp-dependent enzymatically inactive Cre and mScarlet-KASH expression

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   dCre
   

   Use

   AAV

   Tags

   mScarlet-KASH-2A

   Expression

   Mutation

   Enzymatically inactive Cre

   Promoter

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-CREB

   Plasmid

   #68550

   Purpose

   AAV expression of CREB

   Depositor

   Eric Nestler

   Article

   Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

   Insert

   CREB
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMW96

   Plasmid

   #178066

   Purpose

   Expression of His6-SUMO-TEV-GFP-PreScission-γ-TuNA in E. coli

   Depositor

   Tarun Kapoor

   Article

   Wieczorek et al Cell. 2020 Jan 9;180(1):165-175.e16. doi

   Insert

   CDK5RAP2 (CDK5RAP2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9_gRNA1TERTKO_BsagRNA5tertko_2A-GFP

   Plasmid

   #198863

   Purpose

   Plasmid encoding for 2 gRNAs targeting the human TERT gene

   Depositor

   Agnel Sfeir

   Article

   Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

   Insert

   TERT sgRNA (TERT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   u6

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. gh90

   Plasmid

   #106761

   Purpose

   expression of gRNA targeting FUT6

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   FUT6 (FUT6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLENTI-CMV-GFP-TREX1 delta C (aa1-235) BLAST

   Plasmid

   #164229

   Purpose

   lentiviral plasmid for GFP-hTREX1 delta C (aa1-235)

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   TREX1 (TREX1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 235-314

   Promoter

   CMV

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-mCREB

   Plasmid

   #68551

   Purpose

   AAV expression of mCREB

   Depositor

   Eric Nestler

   Article

   Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

   Insert

   mCREB
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S133A

   Promoter

   CMV

   Available Since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pAS_4xEcoLeuT(CUA)_EF1_sfGFP150TAG

   Plasmid

   #174892

   Purpose

   amber suppression reporter sfGFP150TAG with EcoLeuT(CUA) amber suppressor tRNA cassette

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   150TAG in sfGFP

   Promoter

   EF1

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. TERTpWT_Gluc_CCR5

    Plasmid

    #192270

    Purpose

    Donor template for insertion of hTERT promoter driven Gaussia luciferase reporter

    Depositor

    Shantanu Chowdhury

    Article

    Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

    Insert

    Promoter Reporter Insert within donor template
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    TERT promoter driving expression of gaussia

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLENTI CMV GFP-TREX1 delta N (aa236-314) BLAST

    Plasmid

    #164235

    Purpose

    lentiviral plasmid for GFP-TREX1 delta N (aa236-314)

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deletion of amino acids 1-234

    Promoter

    CMV

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAS_FLAG-G1 PylRS

    Plasmid

    #154763

    Purpose

    plasmid with G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+)/GUG-nLuc-3XFLAG-PEST

    Plasmid

    #127314

    Purpose

    Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from GUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG and PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CAGGS-Flex/5'USS-GFP

    Plasmid

    #197884

    Purpose

    Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is reduced without Cre

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pscAAV-Tyr

    Plasmid

    #135616

    Purpose

    Donor vector for genomic targeting of an EcoRI restriction site to the mouse Tyrosinase locus

    Depositor

    Lin He

    Article

    Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

    Insert

    EcoRI restriction site flanked by Tyr homology arms (Tyr Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS_4xBstTyrT(CUA)_EF1 sfGFP150TAG

    Plasmid

    #174891

    Purpose

    amber suppression reporter sfGFP150TAG expression, with BstTyr(CUA) amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG in sfGFP

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKG139

    Plasmid

    #69755

    Purpose

    Retroviral expression of CENP-C

    Depositor

    Iain Cheeseman

    Article

    Gascoigne et al Cell. 2011 Apr 29;145(3):410-22. doi: 10

    Insert

    CENP-C (CENPC Human)
    

    Use

    Retroviral

    Tags

    mCherry-TEV-S

    Expression

    Mammalian

    Mutation

    L341F, N570D relative to NP_001803.2 (numbering b…

    Promoter

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLPC-NMYC-mTRF1

    Plasmid

    #64162

    Purpose

    Retroviral vector expressing mouse TRF1 with N-terminal Myc tag

    Depositor

    Titia de Lange

    Article

    Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

    Insert

    mTRF1 (Terf1 Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

19. Bxb1_miRFP703

    Plasmid

    #183759

    Purpose

    Bxb1-specific donor plasmid for intergrating a transcription unit for expression of membrane-localised miRFP703

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    miRFP703
    

    Use

    Tags

    Lck lipidation signal

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EnhancedSynapsin-DIO-TVA950-EYFP-WPRE

    Plasmid

    #120269

    Purpose

    Allows for initial cellular transduction of EnvA-pseudotyped Rb via Cre-dependent expression of an avian TVA receptor fused with yellow fluorescent protein

    Depositor

    Edward Callaway

    Article

    Faget et al Cell Rep. 2016 Jun 21;15(12):2796-808. d

    Insert

    eSyn-DIO-TVA950:YFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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