164,802 results

1. pDM1383

   Plasmid

   #207423

   Purpose

   Extrachromosomal expression HSPC300-GFP and mCherry-Hs Raf1 (1–134 RBD)

   Depositor

   Rob Kay

   Article

   Veltman et al Elife. 2016 Dec 13;5:e20085. doi: 10.755

   Inserts

   HSPC300
   Raf1 (RAF1 Human)
   

   Use

   Unspecified

   Tags

   GFP and mCherry

   Expression

   Mutation

   aa 1–134 (RBD) only

   Promoter

   Available Since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. XX01CAPN1A-c001

   Plasmid

   #175491

   Purpose

   Protein expression in bacterial cells. Entire CDS, M1-A714 (CAPN1); EF hand 1-5, H86-S268 (CAPNS1). CAPN1 can be biotinylated. Codon optimised.

   Depositor

   Opher Gileadi

   Insert

   CAPN1 / CAPNS1 (CAPN1 Human)
   

   Use

   Tags

   AviTag and His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-eGFP-pA

   Plasmid

   #107281

   Purpose

   donor vector backbone with constitutive eGFP

   Depositor

   Knut Woltjen

   Article

   Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

   Insert

   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRT043-CLYBL-DDdcas9-VPH

   Plasmid

   #158091

   Purpose

   Inducible expression of dCas9-VPH from the CLYBL locus for CRISPR activation

   Depositor

   Martin Kampmann

   Article

   Tian et al Nat Neurosci. 2021 May 24. pii: 10.1038/

   Insert

   CLYBL-CAG-DDdCas9VPH-T2A-GFP
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   June 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV FLEX Synaptophysin GFP

   Plasmid

   #137188

   Purpose

   Cre-dependent expression of synaptophysin-GFP

   Depositor

   Matthew Nolan

   Article

   Surmeli et al Neuron. 2016 Nov 23;92(4):929. doi: 10.1

   Insert

   Synaptophysin GFP (Syp Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRSF-G1mCNPRS

   Plasmid

   #174718

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-4-pyridyl)alanine (mCNP), into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Abdelkader et al Angew Chem Int Ed Engl. 2022 Jan 31. doi

   Insert

   tRNA synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSR33.4r

   Plasmid

   #63198

   Purpose

   Expresses Cph8, Ho1, and PcyA constitutively

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

   Inserts

   Cph8
   Ho1
   PcyA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p5xATF6-GL3

   Plasmid

   #11976

   Purpose

   Reporter gene with five ATF6 sites that is highly sensitive to ER stress

   Depositor

   Ron Prywes

   Article

   Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2

   Insert

   5xATF6 binding site
   

   Use

   Tags

   Luc and c-fos promoter

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mTAGBFP2 Peroxisome visual barcode

   Plasmid

   #158668

   Purpose

   tagging clonal populations with Peroxisomal mTAGBFP2 fluorescent protein. Adding Peroxisome localization signal to mTAGBFP2 WC

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   mTAGBFP2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pU6-mir451 EF1Alpha-puro-T2A-BFP

    Plasmid

    #164792

    Purpose

    Express miR-451 under the U6 promoter in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    microRNA 451a (MIR451A Human)
    puro-T2A-BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha and U6

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMRE-Tn5-160

    Plasmid

    #118540

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    TagBFP2
    

    Use

    Minitn5 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEHIShqprt

    Plasmid

    #92166

    Purpose

    Plasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vector

    Depositor

    James Naismith, Huanting Liu

    Article

    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

    Insert

    Quinolinate Phosphoribosyltransferase (QPRT Human)
    

    Use

    Tags

    TEV protease -cleavable N-terminal 6His

    Expression

    Bacterial

    Mutation

    changed Asp 2 to Asn to create BspHI site

    Promoter

    T7

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Tol2_B-act_dCas9-Vp64-T2A-Citrine

    Plasmid

    #200248

    Purpose

    Zebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesis

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Insert

    dCas9-Vp64-T2A-Citrine
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p20StAD

    Plasmid

    #190268

    Purpose

    Constitutively expresses a protein of interest fused to a GAL4 transcription activating domain. Targeted integration to genomic locus YPRC-delta15, Chromosome XVI.

    Depositor

    Assaf Mosquna

    Article

    Zimran et al ACS Synth Biol. 2022 Aug 19;11(8):2880-2

    Type

    Empty backbone

    Use

    Synthetic Biology; Yeast genome-integrating vector

    Tags

    GAL4-AD

    Expression

    Mutation

    Promoter

    TDH3

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCytERM-mTagBFP2

    Plasmid

    #184940

    Purpose

    ER Visualization (OSER assay) Expresses mTagBFP2 in fusion with N-terminal part of p450

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    CytERM-mTagBFP2
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEBTet-SNAP-Cep72

    Plasmid

    #136819

    Purpose

    Mammalian expression of the centrosomal protein Cep72 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep72 (CEP72 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5/FRT/TO_H2B-HaloTag11-T2A-EGFP

    Plasmid

    #175522

    Purpose

    Nuclear expression of HaloTag11 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag11
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag11 = HaloTag7-M175W

    Promoter

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSOX11-donor

    Plasmid

    #176360

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White, Barbara Stranger

    Insert

    SOX11 (SOX11 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE

    Plasmid

    #162381

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    A25G F286Y

    Promoter

    CAG

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHR_PGK_SNIPR_Hinge Notch SNIPR (Notch2 JMD)

    Plasmid

    #188375

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge