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pCMV-PE7-SB2

Plasmid

#228868

Purpose

PE7 connected to MLH1-SB and MLH1-SB-NLS via 2A sequence

Depositor

Sangsu Bae

Publication

Park et al Cell. 2025 Aug 1:S0092-8674(25)00799-8.

Insert

PE7-2A-MLH1-SB-NLS-2A-MLH1-SB

Use

CRISPR

Available since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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v5 ABE-eVLP

Plasmid

#228467

Purpose

Expresses MMLVgag(C507V)–ABE8e for producing v5 ABE-eVLPs

Depositor

David Liu

Publication

Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

Insert

MMLVgag(C507V)–ABE8e

Tags

FLAG

Expression

Mammalian

Mutation

MMLVgag(C507V)

Promoter

CMV

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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eeBxb1_IVT

Plasmid

#222342

Purpose

Plasmid for evolved and engineered Bxb1 mRNA in vitro transcription template

Depositor

David Liu

Publication

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved and engineered Bxb1

Use

Template for in vitro transcription

Promoter

T7 (inactivated)

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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Cytoplasmic PancACe

Plasmid

#215708

Purpose

Expresses acetyl-CoA biosensor in the cytoplasm

Depositor

Katharine Diehl

Publication

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

PancACe

Use

Lentiviral

Tags

FLAG, HA, and cytoplasmic localization sequence

Expression

Mammalian

Promoter

CMV

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGD-p19

Plasmid

#196326

Purpose

35S promoter-driven expression of the tomato bushy stunt virus p19 RNA silencing suppressor

Depositor

Zhenghe Li

Publication

Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

Insert

tomato bushy stunt virus p19

Expression

Plant

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBR322-PVCpnf17-22

Plasmid

#198272

Purpose

Unmodified pPayload; contains WT PVC payloads/regulatory genes

Depositor

Feng Zhang

Publication

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf17-22

Expression

Bacterial

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p3xnls-mScarlet3_N1

Plasmid

#189775

Purpose

Construct for labelling cell nuclei in mammalian cells using nuclear targeted mScarlet3

Depositor

Dorus Gadella

Publication

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

3xnls-mScarlet3

Expression

Mammalian

Promoter

CMV

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAV-EFS-SauriABE8e-bGH-U6-sgRNA-BsmBI

Plasmid

#189925

Purpose

AAV genome encoding SauriABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

Depositor

David Liu

Publication

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

SauriABE8e

Use

AAV

Mutation

SauriCas9 D15A

Promoter

EFS

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSBbi 41BBL-CD86 mIL-15

Plasmid

#183245

Purpose

Expression of human 41BBL, CD86, and membrane bound human IL-15 (IL-15-IL-15Ra) in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

Depositor

Alexander McLellan

Publication

Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

Insert

41BBL, CD86, mbIL-15 (IL-15-IL-15Ra)

Expression

Mammalian

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP

Plasmid

#176015

Purpose

Mammalian expression of EGFP. Parton lab clone DFS

Depositor

Rob Parton

Publication

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

EGFP

Expression

Mammalian

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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PEA1-Puro

Plasmid

#171991

Purpose

Delivers all prime editing nickase components in a single, puromycin selectable plasmid

Depositor

Paul Thomas

Publication

Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

Insert

CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)

Expression

Mammalian

Promoter

CMV for Cas9, U6 for gRNAs

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNeae2

Plasmid

#168300

Purpose

Bacterial surface display of polypeptides (nanobodies) in E. coli

Depositor

Luis Ángel Fernández

Publication

Salema et al PLoS One. 2013 Sep 23;8(9):e75126. doi:

Insert

Enterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus

Tags

6xHis, E-tag, and myc-tag

Expression

Bacterial

Promoter

LacI-Plac

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE

Plasmid

#125712

Purpose

Expresses an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

Depositor

Ofer Yizhar

Publication

Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

Has service

AAV1, AAV5, and AAV Retrograde

Insert

eOPN3

Use

AAV

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Mutation

deleted amino acids 331-429

Promoter

short version of αCamKinase II promoter (0.4kb)

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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RED

Plasmid

#139835

Purpose

Expresses RNase H1-EGFP- dCas9 fusion protein in human cells

Depositor

Karim Mekhail

Publication

Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:

Insert

RNase H1-EGFP-dCas9 (RNASEH1 RNase H1 (H. sapiens), dCas9 (S. pyogenes))

Expression

Mammalian

Mutation

dCas9 protein contains two mutations: D10A and H8…

Promoter

tet-inducible CMV

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE-NLS-dPspCas13b-3xmRuby3-NLS

Plasmid

#132405

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Expression

Mammalian

Mutation

H133A; H1059A

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.SFFV/TMPRSS2 (variant 1).IRES-neo.WPRE (MT130)

Plasmid

#145843

Purpose

Lentiviral vector genome to ectopically express human TMPRSS2 variant 1

Depositor

Caroline Goujon

Publication

Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

Insert

human TMPRSS2 variant 1 (TMPRSS2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

G8V

Promoter

SFFV

Available since

May 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLX_lenti_osTir1_9Myc_P2A_Bsd

Plasmid

#139746

Purpose

lentiviral vector expressing rice E3 ligase Tir1 in mammalian cells with selection marker

Depositor

Robert Tjian

Publication

Xie et al Nat Methods. 2020 Apr;17(4):430-436. doi

Insert

rice E3 ligase Tir1 (osTir1)

Expression

Mammalian

Promoter

EF1alpha

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP15

Plasmid

#141268

Purpose

Gateway-compatible Entry vector, with insert of NSP15 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP15 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR223 SARS-CoV-2 NSP8

Plasmid

#141262

Purpose

Gateway-compatible Entry vector, with insert of NSP8 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP8 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR223 SARS-CoV-2 NSP7

Plasmid

#141261

Purpose

Gateway-compatible Entry vector, with insert of NSP7 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP7 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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