We narrowed to 7,672 results for: CCH

1. pOBD2 Pab1 E181R (123-204)

   Plasmid

   #196835

   Purpose

   Expresses Pab1 RRM2 fragment E181R fused to GAL4 DNA binding domain

   Depositor

   Stan Fields

   Article

   Melamed et al PLoS Genet. 2015 Feb 11;11(2):e1004918.

   Insert

   Pab1 (PAB1 Budding Yeast)
   

   Tags

   Gal4

   Expression

   Yeast

   Mutation

   123-204 amino acids & E181R

   Promoter

   ADH

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15b I-SceI (Ctermhis) K223I

   Plasmid

   #195615

   Purpose

   Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K223I mutation that results in nicking activity

   Depositor

   Frederick Gimble

   Article

   Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

   Insert

   I-SceI
   

   Expression

   Bacterial

   Mutation

   C2694G, G2345C, G2351C, A3365T, A3366T

   Promoter

   T7

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET15b I-SceI (Ctermhis) K122I

   Plasmid

   #195614

   Purpose

   Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K122I mutation that results in nicking activity

   Depositor

   Frederick Gimble

   Article

   Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

   Insert

   I-SceI
   

   Expression

   Bacterial

   Mutation

   C2458G, A2826T, A2827T

   Promoter

   T7

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET PI-SceI C-His K301A

   Plasmid

   #195611

   Purpose

   Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K301A active site mutation

   Depositor

   Frederick Gimble

   Article

   He et al J Biol Chem. 1998 Feb 20;273(8):4607-15.

   Insert

   PI-SceI
   

   Expression

   Bacterial

   Mutation

   A822G, A823C, A824T

   Promoter

   T7

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. KAN(G418)-TET-OFF – ubiquitin – LEU – 4MYC

   Plasmid

   #193318

   Purpose

   Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   Ubiquitin - Leucine - 4MYC N-terminal tagging
   

   Tags

   4MYC

   Expression

   Bacterial

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. KAN(G418)-TET-OFF – ubiquitin – LEU – 4FLAG

   Plasmid

   #193317

   Purpose

   Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   Ubiquitin - Leucine - 4FLAG N-terminal tagging
   

   Tags

   4FLAG

   Expression

   Bacterial

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET PI-SceI C-His

   Plasmid

   #195610

   Purpose

   Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter with a carboxyl-terminal histidine tag

   Depositor

   Frederick Gimble

   Article

   He et al J Biol Chem. 1998 Feb 20;273(8):4607-15.

   Insert

   PI-SceI
   

   Expression

   Bacterial

   Mutation

   T825C, C900G, A901C, A903C, T1131G, T1137C, T1227…

   Promoter

   T7

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Linked Free NES (M)

   Plasmid

   #182436

   Purpose

   Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Insert

   FPPS(M)-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Expression

   Yeast

   Promoter

   GAL10

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Coexpressed Free NES (M)

   Plasmid

   #182437

   Purpose

   Yeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Inserts

   FPPS(M)
   NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Expression

   Yeast

   Promoter

   GAL10 and GAL7

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Hygro

    Plasmid

    #167899

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Mutation

    D10A, D839A, H840A, N863A

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EBFP2

    Plasmid

    #167897

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Mutation

    D10A, D839A, H840A, N863A

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

12. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Puro

    Plasmid

    #167903

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Mutation

    D10A, D839A, H840A, N863A

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

13. pMM4_14

    Plasmid

    #183062

    Purpose

    Plasmid expressing a biosensor for acetic acid: BM3R1-Haa1-mTurquoise2 under RET2p and mCherry under HREsYGP1p with flanks for genome integration into HO locus

    Depositor

    Yvonne Nygård

    Article

    Mormino et al Microb Cell Fact. 2022 Oct 15;21(1):214.

    Inserts

    mCherry
    BM3R1-HAA1-mTurquoise2
    

    Use

    Synthetic Biology

    Tags

    mCherry and mTurquoise2

    Expression

    Yeast

    Promoter

    HREsYGP1p and RET2

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pILGFP4D41

    Plasmid

    #185841

    Purpose

    Testing the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJCble

    Plasmid

    #185861

    Purpose

    The CRISPR/Cas9-and-gRNA-expressing plasmid with a phleomycin-resistent selectable marker

    Depositor

    Claudia Vickers

    Article

    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

    Insert

    PTDH3-Cas9(N); PSNR52-srRNA-TSUP4; ble
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pILGFP10CF72 (B11)

    Plasmid

    #185859

    Purpose

    Testing the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2M3(2*PZ4)>(BamHI)yEGFP>TURA3
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pILGFP8EFA

    Plasmid

    #185854

    Purpose

    Testing a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3
    

    Expression

    Yeast

    Mutation

    DHFR P66L

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. KBB221

    Plasmid

    #185071

    Purpose

    SST::ACT2 "SA236"

    Depositor

    Ken Belanger

    Article

    Belanger et al Planta. 2003 Oct;217(6):931-50. doi: 10.

    Insert

    ACT2
    

    Expression

    Yeast

    Mutation

    ACT2-SA236

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. KBB215

    Plasmid

    #185068

    Purpose

    SST::WAK2 EcoRI/ScaI fragment (secretion confirmed)

    Depositor

    Ken Belanger

    Article

    Belanger et al Planta. 2003 Oct;217(6):931-50. doi: 10.

    Insert

    WAK2
    

    Expression

    Yeast

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. KBB430

    Plasmid

    #185091

    Purpose

    NMA111-GFP with first nuclear localization signal mutated (amino acids 28 - 30, KRK -> AAA)

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    First nuclear localization signal mutated (amino …

    Available Since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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