We narrowed to 9,173 results for: Pol

1. pX028

   Plasmid

   #167151

   Purpose

   MoClo-compatible Level 1 (position 2) vector encoding Neonothopanus nambi hispidin synthase nnHispS under control of 0.4 kb 35S promoter, for expression in plants

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   p35s-nnHispS-ocsT
   

   Expression

   Plant

   Promoter

   p35s

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-2T-TRR-C421-Y2404F

   Plasmid

   #182846

   Purpose

   mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG induction

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2014 Mar;141(5):1129-39. do

   Insert

   TRR 2011-2431 (trr Fly)
   

   Expression

   Bacterial

   Mutation

   mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-DN-RFX

   Plasmid

   #52296

   Purpose

   Dominant-negatively inhibits RFX (regulatory factor X) transcription factor. Made of mouse RFX1 DNA binding domain (400-527 aa) and C-terminal myc tag.

   Depositor

   Christopher A Walsh

   Article

   Bae et al Science. 2014 Feb 14;343(6172):764-8. do

   Insert

   Dominant negative RFX
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5 GST MetaP2

   Plasmid

   #112750

   Purpose

   express HA-GST-FRB-MetaP2

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   HA-GST-FRB-MetaP2 (METAP2 Human)
   

   Tags

   GST

   Expression

   Mammalian

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQCXIB Flag MFF WT

   Plasmid

   #74384

   Purpose

   Retroviral expression vector containing Flag tagged WT MFF isoform 5

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   Flag-MFF (MFF Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 10, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pX018

   Plasmid

   #167144

   Purpose

   MoClo-compatible Level 0-SP promoterless vector encoding Neonothopanus nambi luciferase nnLuz codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   fungal luciferase, nnLuz
   

   Use

   Luciferase and Synthetic Biology

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF

   Plasmid

   #119864

   Purpose

   Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

   Insert

   BirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Tags

   HA-tagged biotin ligase (BirA), Thosea asigna vir…

   Expression

   Bacterial

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST27-GST-ATXN1 FL(30Q)-S776D

   Plasmid

   #21755

   Depositor

   Huda Zoghbi

   Article

   Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Tags

   GST

   Expression

   Mammalian

   Mutation

   insert contains a stretch of 32 amino acids (30 Q…

   Promoter

   CMV

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pX029

   Plasmid

   #167152

   Purpose

   MoClo-compatible Level 1 (position 3) vector encoding Neonothopanus nambi hispidin-3-hydroxylase nnH3H under control of 0.4 kb 35S promoter, for expression in plants

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   p35s-nnH3H-ocsT
   

   Expression

   Plant

   Promoter

   p35s

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX019

    Plasmid

    #167145

    Purpose

    MoClo-compatible Level 0-CDS1 promoterless vector encoding Neonothopanus nambi hispidin-3-hydroxylase nnH3H codon-optimised for expression in Nicotiana benthamiana

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    fungal hispidin-3-hydroxylase, nnH3H
    

    Use

    Synthetic Biology

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Frt-V5-hspB3

    Plasmid

    #63104

    Purpose

    Expression of HSPB3 (small HSP) tagged with V5 in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb3 (HSPB3 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLJM1 Flag GFP 4E-BP1 Omp25

    Plasmid

    #112760

    Purpose

    express GFP-4EBP1-Omp25

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-GFP-4EBP1-Omp25 (EIF4EBP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag eGFP and Omp25

    Available Since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3 Flag MFF iso1 S172A

    Plasmid

    #74391

    Purpose

    Flag tagged human S172A MFF isoform 1 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    delta exon 1 (1-26) and Ser 172 Ala.

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

14. PCRII apoEa

    Plasmid

    #121900

    Purpose

    Plasmid for making zebrafish apoEa in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    ApoEa (apoea Zebrafish)
    

    Use

    Unspecified

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pScalps_puro_mIL-2Rα RK

    Plasmid

    #59920

    Purpose

    Expresses mouse interleukin-2 receptor alpha chain mutated

    Depositor

    Silvia Monticelli

    Article

    Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

    Insert

    interleukin 2 receptor alpha (Il2ra Mouse)
    

    Use

    Lentiviral

    Mutation

    changed sequence of the intracellular (C-terminal…

    Available Since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

16. psicheck-2-LPPRC

    Plasmid

    #48167

    Purpose

    contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    LRPPRC leucine rich pentatricopeptide repeat containing (Lrpprc Human)
    

    Use

    Luciferase

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

17. CRY-GalVP16l (B694)

    Plasmid

    #92030

    Purpose

    expresses CRY2 (Full length, mutant NLS) fused to Gal4DNA (residues 1-147) fused to VP16 activation domain (long form)

    Depositor

    Chandra Tucker

    Article

    Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Tags

    GalBD-VP16

    Expression

    Mammalian

    Mutation

    NLS sequence of CRY2 mutated

    Promoter

    CMV

    Available Since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCR2.1-INS-904

    Plasmid

    #53970

    Purpose

    904 bp insert from human INS gene used as a control for methylation-specific PCR assays

    Depositor

    Kevin Ferreri

    Article

    Husseiny et al PLoS One. 2012;7(10):e47942. doi: 10.137

    Insert

    human INS gene partial (INS Human)
    

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3 NDUFS6(5’UTR)-FF

    Plasmid

    #85488

    Purpose

    Firefly luciferase under the control of NDUFS6 5'UTR

    Depositor

    Ivan Topisirovic

    Article

    Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

    Insert

    5'UTR of NDUFS6 (NDUFS6 Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Available Since

    Feb. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3 Flag MFF iso4 S172A

    Plasmid

    #74393

    Purpose

    Flag tagged human S172A MFF isoform 4 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 172 Ala (numbering based on MFF isoform 1)

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only