We narrowed to 24,446 results for: lars

1. dGFP-Granuphilin

   Plasmid

   #170868

   Purpose

   Mammalian expression of dGFP-Granuphilin.

   Depositor

   Justin Taraska

   Article

   Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

   Insert

   Synaptotagmin-like 5 (SYTL5 Human)
   

   Use

   Tags

   dEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCh-LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)

   Plasmid

   #165029

   Purpose

   Express cpFRB1-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry

   Depositor

   Yubin Zhou

   Article

   Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

   Insert

   LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GFP-LaM(N)-cpFRB1-T2A-FKBP-LaM(C)

   Plasmid

   #165026

   Purpose

   Express cpFRB1-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFP

   Depositor

   Yubin Zhou

   Article

   Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

   Insert

   LaM8(N)-cpFRB1-T2A-FKBP-LaM8(C)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GFP-LaM(N)-cpFRB2-T2A-FKBP-LaM(C)

   Plasmid

   #165028

   Purpose

   Express cpFRB2-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFP

   Depositor

   Yubin Zhou

   Article

   Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

   Insert

   LaM8(N)-cpFRB2-T2A-FKBP-LaM8(C)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET15b/GVcl 1-1066 /*TBM

   Plasmid

   #162781

   Purpose

   amplification of Gallus gallus Vcl mutated in Tankyrase Binding Domain II

   Depositor

   Laura Lafon-Hughes

   Article

   et al PeerJ

   Insert

   Gallus gallus vinculin (VCL Chicken)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changed Gly 454 to Val

   Promoter

   Available since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Adeno-miR-23b

   Plasmid

   #112538

   Purpose

   Adenovirus overexpression

   Depositor

   Shusheng Wang

   Article

   Zhou et al Proc Natl Acad Sci U S A. 2011 May 17;10

   Insert

   miR-23b (MIR23B Human)
   

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GP821

   Plasmid

   #164552

   Purpose

   Expresses Smo-FLAG K434R

   Depositor

   Gregory Pazour

   Article

   Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Insert

   Smoothened K434R (Smo Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   One of the three Lysines in loop three of Smoothe…

   Promoter

   GgCryD1

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. GP823

   Plasmid

   #164554

   Purpose

   Expresses Smo-FLAG K448R

   Depositor

   Gregory Pazour

   Article

   Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Insert

   Smoothened K448R (Smo Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   One of the three Lysines in loop three of Smoothe…

   Promoter

   GgCryD1

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GP822

   Plasmid

   #164553

   Purpose

   Expresses Smo-FLAG K444R

   Depositor

   Gregory Pazour

   Article

   Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Insert

   Smoothened K444R (Smo Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   One of the three Lysines in loop three of Smoothe…

   Promoter

   GgCryD1

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GP806

    Plasmid

    #164534

    Purpose

    Expresses Smo noK-FLAG-Ub

    Depositor

    Gregory Pazour

    Article

    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    Smoothened noK (Smo Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG-Ub

    Expression

    Mutation

    All 21 cytoplasmic Lysines of Smoothened changed …

    Promoter

    CMV

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAGM9121_Strep II

    Plasmid

    #153512

    Purpose

    Strep II C-terminal Tag Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Strep II
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAGM9121_TwinStrep

    Plasmid

    #153513

    Purpose

    TwinStrep C-terminal Tag Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    TwinStrep
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAGM9121_Aaw-Glucoamylase SP

    Plasmid

    #153497

    Purpose

    Aspergillus awamori Glucoamylase Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Glucoamylase SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAGM9121_Ani- α Amylase SP

    Plasmid

    #153503

    Purpose

    Aspergillus niger α Amylase Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    α Amylase SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAGM9121_Sce- α Galactosidase SP

    Plasmid

    #153504

    Purpose

    α Galactosidase Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    α Galactosidase SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAGM9121_Gga-Lysozym SP

    Plasmid

    #153505

    Purpose

    Chicken Lysozym Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Lysozym SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPAP003

    Plasmid

    #153490

    Purpose

    Level 2 integrative plasmid for stable expression in P. pastoris

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAGM9121_Sce-Prepro SP

    Plasmid

    #153491

    Purpose

    S. cerevisiae Prepro Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Prepro SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAGM9121_Sce-Invertase 2 SP

    Plasmid

    #153493

    Purpose

    S. cerevisiae Invertase Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Invertase 2 SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCL21-decoder

    Plasmid

    #159448

    Purpose

    Insert: Pact1-lexA-ER-B112-phosphodegron-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is degraded upon addition of alpha factor. Backbone: pRG201 (addGene). Marker: Met15.

    Depositor

    Joerg Stelling

    Article

    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

    Insert

    Pact1-lexA-ER-B112-phosphodegron-tCyc1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    act1

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCL33-decoder

    Plasmid

    #159451

    Purpose

    Insert: Pfus1mut-tetR-nls-malE-tCyc1. TetR-NLS-MBP (Azizoglu et al, bioRxiv 2019) is inducible by alpha-factor. Backbone: pRG203MX (addgene). Marker: His3.

    Depositor

    Joerg Stelling

    Article

    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

    Insert

    Pfus1mut-tetR-nls-malE-tCyc1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Pfus1mut

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCL38-decoder

    Plasmid

    #159452

    Purpose

    Insert: Pfus1tet-lexA-ER-B112-tCyc1.The transcription factor LexA-ER-B112 (see addgene FRP880) is inducible by alpha factor, and repressible by TetR. Backbone: pRG235 (addgene). Marker: Leu2

    Depositor

    Joerg Stelling

    Article

    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

    Insert

    Pfus1tet-LexA-ER-B112-tCyc1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Pfus1tet

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCL12-decoder

    Plasmid

    #159447

    Purpose

    Insert: PlexAtet-citrine-tCyc1. The fluorescent protein Citrine is inducible by LexA-ER-B112 upon addition of estradiol, and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.

    Depositor

    Joerg Stelling

    Article

    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

    Insert

    PlexAtet-Citrine-tCyc1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PlexAtet

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. plIVMD118

    Plasmid

    #160938

    Purpose

    Excess Capsid Protein in vivo mRNA display

    Depositor

    Saeed Tavazoie

    Article

    Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

    Insert

    MCP-mCherry-FLAG tag
    

    Use

    Tags

    MYC tag

    Expression

    Yeast

    Mutation

    Promoter

    MET25

    Available since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. plIVMD115

    Plasmid

    #160937

    Purpose

    Excess Capsid Protein for in vivo mRNA display

    Depositor

    Saeed Tavazoie

    Article

    Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

    Insert

    MCP-mCherry-MYC tag
    

    Use

    Tags

    MYC tag

    Expression

    Yeast

    Mutation

    Promoter

    MET25

    Available since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-11a (D3 - L39V)

    Plasmid

    #136607

    Purpose

    Expresses DANCER-3 mutant (reversion L39V)

    Depositor

    Roberto Chica

    Article

    Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

    Insert

    DANCER-3 mutant (L39V reversion)
    

    Use

    Tags

    5x-Histag

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: Y3F-L7I-A34F-V54I

    Promoter

    T7 promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHH0103_DLG2_PDZ_3

    Plasmid

    #110001

    Purpose

    Protein expression and purification of DLG2_PDZ_3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    DLG2_PDZ_3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHH0103_SFN_14-3-3

    Plasmid

    #109937

    Purpose

    Protein expression and purification of SFN_14-3-3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    SFN_14-3-3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHH0103_TLN2_IRS

    Plasmid

    #109939

    Purpose

    Protein expression and purification of TLN2_IRS

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    TLN2_IRS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHH0103_CCNE2_Cyclin

    Plasmid

    #109917

    Purpose

    Protein expression and purification of CCNE2_Cyclin

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    CCNE2_Cyclin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge