We narrowed to 11,691 results for: NSI

1. pGEX6P1-sr1(SIRPT1)

   Plasmid

   #75297

   Purpose

   Expresses Sacsin sr1 (SIRPT1 repeat) domain (84-339) in fusion with GST (bacterial)

   Depositor

   Kalle Gehring

   Article

   Menade et al J Biol Chem. 2018 Aug 17;293(33):12832-1

   Insert

   Human Sacsin 84-339
   

   Tags

   GST-Tag

   Expression

   Bacterial

   Available Since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHH0103_UBASH3B-1/1

   Plasmid

   #91462

   Purpose

   Protein expression and purification of human SH3 domain construct UBASH3B-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   UBASH3B-1/1 (UBASH3B Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-REPORT (PGK) 5xGRE-NanoLuc

   Plasmid

   #207170

   Purpose

   GAl4 response element driven NanoLuc Lentiviral reporter

   Depositor

   David Bersten

   Article

   Roennfeldt et al Biomolecules. 2023 Oct 19;13(10):1545. d

   Inserts

   NanoLuciferase
   d2eGFP
   5x GRE
   

   Use

   Lentiviral, Luciferase, and Synthetic Biology

   Tags

   3x SV40 NLS and PEST

   Promoter

   Gal4 response element minP and PGK

   Available Since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCA528 SPASTIN , residues 112-196

   Plasmid

   #180627

   Purpose

   Bacterial expression for SPASTIN MIT, residues 112-196. Has N-term HIS-SUMO tag. Used for X-ray crystallography. Internal ID:WISP21-111.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SPASTIN
   

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 112-196

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXPR_BRD003 sgKEAP1-2

   Plasmid

   #74986

   Purpose

   sgKEAP1-2, puromycin selection

   Depositor

   William Hahn

   Article

   Krall et al Elife. 2017 Feb 1;6:e18970. doi: 10.7554

   Insert

   KEAP1
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBeast-pAhpC-RBS- sfGFP

   Plasmid

   #190051

   Purpose

   GFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoter

   Depositor

   Jerome Bonnet

   Article

   Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

   Insert

   sfGFP
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCold TDP43 G294A

   Plasmid

   #27452

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Tags

   Factor Xa and His6

   Expression

   Bacterial

   Mutation

   Glycine 294 mutated to Alanine (G294A)

   Available Since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

8. RGD-sfGFP-I27-CC

   Plasmid

   #85392

   Purpose

   MTFM sensor to measure integrin mechanical forces. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines (CC) for immobilization and sfGFP for fluorescent labeling

   Depositor

   Khalid Salaita

   Article

   Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:

   Insert

   RGD motif for integrin binding fused to sfGFP and titin immunoglobulin domain (I27)
   

   Tags

   6x His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCA528 SPASTIN , residues 108-200

   Plasmid

   #180628

   Purpose

   Bacterial expression for SPASTIN MIT, residues 108-200. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-10.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SPASTIN
   

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 108-200

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-GFP

    Plasmid

    #75129

    Purpose

    Retroviral expression plasmid encoding shBrd9_1061 (targeting murine Brd9)

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    shBrd9_1061
    

    Use

    Retroviral

    Available Since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pMA7

    Plasmid

    #79967

    Purpose

    TM-MAGE strain

    Depositor

    Morten Sommer

    Article

    Lennen et al Nucleic Acids Res. 2016 Feb 29;44(4):e36

    Inserts

    Lambda Red recombinase beta subunit
    DNA adenine methylase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_12xTEAD_luc2

    Plasmid

    #173004

    Purpose

    firefly luciferase reporter vector with 12x clustered TEAD binding sites

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    12xTEAD-luc2
    

    Use

    AAV and Luciferase

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. psgRNA (GB0645)

    Plasmid

    #68210

    Purpose

    Provides a Short guide RNA which is the combination of the bacterial crRNA and tracrRNA into a single guide transcript; it does not contain any target site

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    SgRNA
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1-blast shGAC

    Plasmid

    #110410

    Purpose

    shGAC (Target CCTCTGTTCTGTCAGAGTT), silence glutaminase isoform GAC, blasticidin selection.

    Depositor

    Sandra Martha Gomes Dias

    Insert

    GLS glutaminase (GLS Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6 (RNA Pol III)

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEX- HcRed -hLC3ΔG

    Plasmid

    #24992

    Depositor

    Isei Tanida

    Article

    Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

    Insert

    a mutant human MAP1-LC3B fused with HcRed (MAP1LC3B Human)
    

    Tags

    HcRed

    Expression

    Mammalian

    Mutation

    deleted Gly at 121

    Available Since

    June 28, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pCMV7.1_3xFLAG-MOB1A

    Plasmid

    #172988

    Purpose

    constitutive expression of FLAG-tagged MOB1A

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-MOB1A (MOB1A Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. tdp43-EGFP construct11

    Plasmid

    #28204

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa193-274 only

    Available Since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-Syn-ChromeQ-GFP

    Plasmid

    #123317

    Purpose

    AAV-mediated expression of ChromeQ-GFP under the Syn promoter.

    Depositor

    Edward Boyden

    Article

    Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

    Insert

    ChromeQ-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Channelrhodopsin-2 (ChR2) with mutations A71S/ E9…

    Promoter

    Syn

    Available Since

    March 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMJA104

    Plasmid

    #128520

    Purpose

    Expresses amyloid human Lysozyme in Pichia pastoris

    Depositor

    Marcos Alcocer

    Article

    Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

    Insert

    Artificial gene for human lysozyme I56T (LYZ Human)
    

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH0103_PIK3R2-1/1

    Plasmid

    #91415

    Purpose

    Protein expression and purification of human SH3 domain construct PIK3R2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    PIK3R2-1/1 (PIK3R2 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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