We narrowed to 11,284 results for: VARS

1. K365-iLid

   Plasmid

   #188456

   Purpose

   Component of optically-induced motor dimerization system

   Depositor

   Zvonimir Dogic

   Article

   Lemma et al PNAS Nexus. 2023 Apr 12;2(5):pgad130. do

   Insert

   Kinesin 1-365 (Khc Fly)
   

   Tags

   6xHis and iLid

   Expression

   Bacterial

   Mutation

   Kinesin amino acids 1-365

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. K365-micro

   Plasmid

   #188457

   Purpose

   Component of optically-induced motor dimerization system

   Depositor

   Zvonimir Dogic

   Article

   Lemma et al PNAS Nexus. 2023 Apr 12;2(5):pgad130. do

   Insert

   Kinesin 1-365 (Khc Fly)
   

   Tags

   6xHis-MBP-TEV and SspB micro

   Expression

   Bacterial

   Mutation

   Kinesin amino acids 1-365

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGBT-RP8.3

   Plasmid

   #189848

   Purpose

   Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 3.

   Depositor

   Stephen Ekker

   Article

   Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   mRFP and tagBFP

   Promoter

   beta Actin

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7-EGFP-C1-HseIF4G1-L640AV646A_AD

   Plasmid

   #148520

   Purpose

   Mammalian Expression of HseIF4G1-L640AV646A

   Depositor

   Elisa Izaurralde

   Article

   Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

   Insert

   HseIF4G1-L640AV646A (EIF4G1 Human)
   

   Expression

   Mammalian

   Mutation

   one non silent mutation M432V (natural variant is…

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT7-EGFP-C1-HseIF4G1-LIVVtoAAAA_AD

   Plasmid

   #148521

   Purpose

   Mammalian Expression of HseIF4G1-LIVVtoAAAA

   Depositor

   Elisa Izaurralde

   Article

   Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

   Insert

   HseIF4G1-LIVVtoAAAA (EIF4G1 Human)
   

   Expression

   Mammalian

   Mutation

   one non silent mutation M432V (natural variant is…

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7-EGFP-C1-HseIF4G1-Y619AL624AL625A_AD

   Plasmid

   #148522

   Purpose

   Mammalian Expression of HseIF4G1-Y619AL624AL625A

   Depositor

   Elisa Izaurralde

   Article

   Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

   Insert

   HseIF4G1-Y619AL624AL625A (EIF4G1 Human)
   

   Expression

   Mammalian

   Mutation

   one non silent mutation M432V (natural variant is…

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. RAT +D122H

   Plasmid

   #187426

   Purpose

   Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al bioRxiv 2021.11.29.470343

   Inserts

   ATP1A1 (Atp1a1 Rat)
   ATP1B1 (Atp1b1 Rat)
   

   Expression

   Insect

   Mutation

   changed aspartic acid at 129 for histidine

   Promoter

   PH and p10

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. RAT +R111E

   Plasmid

   #187425

   Purpose

   Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al bioRxiv 2021.11.29.470343

   Inserts

   ATP1A1 (Atp1a1 Rat)
   ATP1B1 (Atp1b1 Rat)
   

   Expression

   Insect

   Mutation

   changed arginine at 118 to glutamic acid

   Promoter

   PH and p10

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. IL2-mCherry-rGBD in pmCherryN1

   Plasmid

   #187286

   Purpose

   Express IL2-Rhotekin GTPase-binding domain-mCherry

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   interleukin-2 receptor alpha-subunit, Rhotekin (IL2, Rtkn Mouse, Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET15b MOSPD2 [315-445]

    Plasmid

    #186474

    Purpose

    Expression of human MOSPD2 (MSP domain from residue 315 to 445), fused to a cleavable 6HIS tag, in bacteria

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO J. 2020 Dec 1;39(23):e104369. doi:

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    MSP domain [315-445]

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. EGFP-SopB(172-554)

    Plasmid

    #183664

    Purpose

    N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Amino acids 172-554

    Promoter

    CMV

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. EGFP-SopB(211-554)

    Plasmid

    #183665

    Purpose

    N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Amino acids 211-554

    Promoter

    CMV

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B2

    Plasmid

    #165083

    Purpose

    gRNA 2 of pair B for Stx5L-specific knockout

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C1

    Plasmid

    #165085

    Purpose

    gRNA 1 of pair C for Stx5L-specific knockout

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C2

    Plasmid

    #165086

    Purpose

    gRNA 2 of pair C for Stx5L-specific knockout

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTwist-SARS-CoV-2 Δ18 B.1.351v3/wtRBD

    Plasmid

    #169468

    Purpose

    Encodes SARS-CoV-2 B.1.351v3/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.351v3/wtRBD Spike truncated to remove 18 amino acids (S )
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTwist-SARS-CoV-2 Δ18 B.1.351v1/wtRBD

    Plasmid

    #169466

    Purpose

    Encodes SARS-CoV-2 B.1.351v1/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.351v1/wtRBD Spike truncated to remove 18 amino acids (S )
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSico_U6-PGC1a1 sgRNA

    Plasmid

    #165425

    Purpose

    Expression of gRNA against human PGC-1a variant 1

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    gRNA against PGC-1a variant 1 (PPARGC1A Human, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

19. pCMV ShadowY-AURKA-mTurq2

    Plasmid

    #157770

    Purpose

    Expression of AuroraA kinase biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    mTurquoise2 and shadowY

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPGK-T7/2-CD44v2-10 (-cyt)

    Plasmid

    #137825

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v2-10 (-cyt) (CD44 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    without cytoplasmic region - N213S on the CD44 re…

    Promoter

    PGK

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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