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pQCXIP-Pex3-EGFP-APEX2-V5

Plasmid

#236401

Purpose

Expresses a fluorescent peroxisome-localized APEX2

Depositor

Jared Rutter

Article

Cunningham et al J Cell Sci. 2025 Mar 13:jcs.263693. doi:

Insert

Pex3(fragment)-EGFP-APEX2-V5

Use

Retroviral

Tags

APEX2, EGFP, and V5

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pQCXIP-EGFP-ADRP-APEX2-V5

Plasmid

#236405

Purpose

Expresses a fluorescent lipid droplet-localized APEX2

Depositor

Jared Rutter

Article

Cunningham et al J Cell Sci. 2025 Mar 13:jcs.263693. doi:

Insert

EGFP-ADRP-APEX2-V5

Use

Retroviral

Tags

APEX2, EGFP, and V5

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-Cas13bt1-non-targeting (pJK2)

Plasmid

#229054

Purpose

Cloning backbone for Cas13bt1 crRNA (U6 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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phU1-Cas13bt1-non-targeting (pEZ25)

Plasmid

#229055

Purpose

Cloning backbone for Cas13bt1 crRNA (Human U1 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-RanCas13b-non-targeting (pJK6)

Plasmid

#229056

Purpose

Cloning backbone for RanCas13b crRNA (U6 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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phU1-RanCas13b-non-targeting (pEZ45)

Plasmid

#229057

Purpose

Cloning backbone for RanCas13b crRNA (Human U1 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+-Zeo

Plasmid

#221725

Purpose

Expression of Zeocin resistance marker in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

BleoR

Use

Fish expression

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pB4GWnY-MpPHOT

Plasmid

#228472

Purpose

Vector for the expression of MpPHOT fused to the N-terminal half of YFP in plants (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

MpPHOT

Expression

Bacterial and Plant

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB605-MpRTN1

Plasmid

#228475

Purpose

Binary vector for the expression of MpRTN1 fused to the sGFP in plants (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

MpRTN1

Expression

Bacterial and Plant

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pB4GWnY-MpRTN1

Plasmid

#228477

Purpose

Vector for the expression of MpRTN1 fused to the N-terminal half of YFP in plants (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

MpRTN1

Expression

Bacterial and Plant

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pB4GWcY-MpRTN1

Plasmid

#228478

Purpose

Vector for the expression of MpRTN1 fused to the C-terminal half of YFP in plants (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

MpRTN1

Expression

Bacterial and Plant

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pB4GWcY-MpPHOT

Plasmid

#228473

Purpose

Vector for the expression of MpPHOT fused to the C-terminal half of YFP in plants (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

MpPHOT

Expression

Bacterial and Plant

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pNB_AR_Pllaco 1E2crimson

Plasmid

#205216

Purpose

Expresses E2crimson

Depositor

Sangram Bagh

Article

Srivastava et al ACS Synth Biol. 2022 Mar 18;11(3):1040-1

Insert

E2-Crimson

Use

Synthetic Biology

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLPC-C-SF-TAP-DEST-TLK1- Q479E

Plasmid

#218830

Purpose

Expresses C-terminal Strep-FLAG-tagged Q479E human TLK1 variant 5 in a retrovirus vector.

Depositor

Travis Stracker

Insert

TLK1 Q479E (TLK1 Human)

Tags

FLAG-STREP

Expression

Mammalian

Mutation

Q479E

Promoter

CMV

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-5

Plasmid

#221491

Purpose

NKX2-5 lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-5 (NKX2-5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1

Plasmid

#221472

Purpose

Gateway cloning of NKX2-1 short isoform overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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hTspan12(1-305)-1D4_pTT5

Plasmid

#216381

Purpose

Mammalian expression vector to transiently express full-length human Tspan12 with a Rho1D4 tag

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Tetraspanin-12 (TSPAN12 Human)

Tags

Rho1D4

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB11785

Plasmid

#212699

Purpose

gRNA targeting to the intergradtion site E4

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site E4

Expression

Yeast

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB10769

Plasmid

#212694

Purpose

gRNA targeting to the intergradtion site E2

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site E2

Expression

Yeast

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB12719

Plasmid

#212702

Purpose

gRNA targeting to the intergradtion site F3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site F3

Expression

Yeast

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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