We narrowed to 7,168 results for: GFP expression plasmids

1. NaChBac pTracer CMV2

   Plasmid

   #60835

   Purpose

   Mammalian expression plasmid containing NaChBac gene with an EGFP reporter

   Depositor

   David Clapham

   Article

   Ren et al Science. 2001 Dec 14;294(5550):2372-5.

   Insert

   NaChBac
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

2. ρ1-EM GABAA receptor in pEZT-BM

   Plasmid

   #213072

   Purpose

   Express human GABA-A rho1 receptor with truncated intracellular domain and superfolder GFP insertion in ICD

   Depositor

   Erik Lindahl

   Article

   Cowgill et al Neuron. 2023 Nov 1;111(21):3450-3464.e5.

   Insert

   Human GABAa rho1 receptor (GABRR1 Human)
   

   Use

   Bacmam, baculovirus, oocyte expression

   Tags

   Twin-Strep tag and superfolderGFP

   Expression

   Mammalian

   Mutation

   Encodes residues S58–L383 and D451–S479, separate…

   Promoter

   CMV and T7

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PAJ379

   Plasmid

   #104143

   Purpose

   Expresses 3A igRNA and sfGFP

   Depositor

   Alfonso Jaramillo

   Article

   Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. PAJ407

   Plasmid

   #104144

   Purpose

   Expresses 3A_v9 igRNA and sfGFP

   Depositor

   Alfonso Jaramillo

   Article

   Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. PAJ426

   Plasmid

   #104145

   Purpose

   Expresses HIV3 igRNA and sfGFP

   Depositor

   Alfonso Jaramillo

   Article

   Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-SFFV-ZFYVE27-WPRE-UbC-Emerald

   Plasmid

   #214469

   Purpose

   Lentiviral vector plasmid expressing human zinc finger FYVE-type containing 27 (ZFYVE27) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis

   Inserts

   ZFYVE27 (ZFYVE27 Human)
   Emerald
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEPQDKN0248

   Plasmid

   #162313

   Purpose

   Reporter plasmid for T7-driven E. coli cell-free protein synthesis with detection via HiBiT

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Insert

   T7prom-RBS-sfGFP-HiBiT
   

   Use

   Synthetic Biology

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEPQDKN0332

   Plasmid

   #162314

   Purpose

   Reporter plasmid for TNT SP6 Coupled Wheat Germ Extract System with detection via HiBiT

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Insert

   SP6prom-EO1-sfGFP-HiBiT
   

   Use

   Synthetic Biology

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.U6.shRNA-Sdc1-1376

   Plasmid

   #195574

   Purpose

   Expresses shRNA to knockdown mouse Syndecan-1

   Depositor

   Kevin Park

   Article

   Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

   Insert

   shRNA, eGFP (Sdc1 Synthetic)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Lyn-dPlcR(K30/32L)

    Plasmid

    #181833

    Purpose

    Phospholipid binding-deficient Lyn-dPlcR negative-control plasmid.

    Depositor

    Jin Zhang

    Article

    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

    Insert

    Lyn-dPlcR (K30L,K32L)
    

    Tags

    N-terminal targeting sequence from Lyn kinase, dd…

    Expression

    Mammalian

    Mutation

    Lysines 30 and 32 in PH domain mutated to leucine.

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. BNLS

    Plasmid

    #50836

    Purpose

    Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP B
    

    Tags

    triplicated NLS sequence DPKKKRKV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. sTR056

    Plasmid

    #177369

    Purpose

    Toehold switch, with an unpaired toehold region at the 5′-end that interacts with the trigger RNA (sTR060, Addgene plasmid # 177370) to unfold the switch.

    Depositor

    Jerome Bonnet, Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Insert

    sfGFP
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. LRG-sgRNA.FOXA-ZEB2_CRISPRd_v2

    Plasmid

    #216173

    Purpose

    Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Insert

    sgRNA- FOXA.bs- ZEB2.locus
    

    Use

    Lentiviral

    Tags

    EGFP

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. SECURE miniABEmax-K20A/R21A (pJUL1774)

    Plasmid

    #131312

    Purpose

    CMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. hA3A-BE3 (pRZ215)

    Plasmid

    #131314

    Purpose

    CAG promoter expression plasmid for hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. hAID-BE3 (pRZ352)

    Plasmid

    #131316

    Purpose

    CAG promoter expression plasmid for hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. PX458-3xHA-en31FnCas9

    Plasmid

    #201949

    Purpose

    Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    3xHA-NLS-en31FnCas9-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    G1243T, E1369R, E1603H on FnCas9

    Promoter

    Cbh

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. PX458-3xHA-en1FnCas9

    Plasmid

    #201947

    Purpose

    Mammalian expression plasmid of enhanced activity FnCas9 variant en1FnCas9 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    3xHA-NLS-en1FnCas9-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    E1369R on FnCas9

    Promoter

    Cbh

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. PX458-3xHA-en15FnCas9

    Plasmid

    #201948

    Purpose

    Mammalian expression plasmid of enhanced activity FnCas9 variant en15FnCas9 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    3xHA-NLS-en15FnCas9-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    E1603H on FnCas9

    Promoter

    Cbh

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. PX458-3xHA-FnCas9ABEmax8.17d

    Plasmid

    #201955

    Purpose

    Mammalian expression plasmid of FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    bpNLS-FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D11A on FnCas9, V82S, V106W, Q154R on mutant TadA

    Promoter

    Cbh

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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