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Plasmid#226964PurposeCBh-eSpCas9(1.1)-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only
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PX459v3-eSpCas9(1.1)
Plasmid#178800PurposeHigh-fidelity eSpCas9(1.1) with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCbh (Cas9-2A Puro) and U6 (gRNA)Available sinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX459v3-SpCas9-HF1
Plasmid#178801PurposeHigh-fidelity SpCas9-HF1 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCbh (Cas9-2A Puro) and U6 (gRNA)Available sinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-SaKKH-spA-miniU6-miniSdd6-MmHPD-T2
Plasmid#204852PurposeAAV vector for cytosine base editing using miniSdd6 at HPD-T2 target site in mouseDepositorInsertminiSdd6-nSaCas9KKH(D10A)-UGI
UseAAV and CRISPRTagsExpressionMutationD10A, E782K, N968K, R1015H in SaCas9PromoterEFS, miniU6Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH15
Plasmid#125800PurposeLevel0 StCas9, Arabidopsis codon optimizedDepositorInsertSaCas9, Arabidopsis codon optimized
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
p426*-SaSgH
Plasmid#136994PurposeHelper plasmid for SaCas9 gRNA cloning and expressionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterSNR52Available sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSPneoSagRNAH
Plasmid#123265PurposeExpresses gRNA of Staphylococcus aureus Cas9 (SaCas9) in LeishmaniaDepositorTypeEmpty backboneUseCRISPR; Leishmania expressionTagsExpressionMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNeoSagRNAH
Plasmid#123266PurposeExpresses gRNA of Staphylococcus aureus Cas9 (SaCas9) in LeishmaniaDepositorTypeEmpty backboneUseCRISPR; Leishmania expressionTagsExpressionMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
Mammalian_SaAID
Plasmid#188653PurposeExpresses SaAID in mammalian cellsDepositorInsertnSaCas9-AID-UGI
UseTagsUGIExpressionMammalianMutationD10A for SaCas9PromoterpCMVAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0040
Plasmid#117607PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_NbPDS(pEPOR1CB0058)DepositorInsert[35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only