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Showing: 621 - 640 of 7181 results

  3. Lenti-EF1alpha-dCas9-KRAB_Hygro

    Plasmid
    #192663
    Purpose
    3rd generation lenti vector encoding dCas9-KRAB with 2A Hygro resistance marker
    Depositor
    Jeffrey Miner
    Insert
    dCas9-KRAB
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A, D839A, H840A and N863A in Cas9
    Promoter
    EF1a
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192663-plasmid-map-sequence-id-379915

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  10. LMP AMPK alpha1 hp1

    Plasmid
    #89489
    Purpose
    shRNA against AMPK alpha1 in mouse/human to knock down protein expression
    Depositor
    Russell Jones
    Article
    Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi
    Insert
    hairpin against mouse/human AMPKalpha1 (PRKAA1, Prkaa1 Human, Mouse)
    Use
    Mouse Targeting and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (The GFP-Puro is from a PGK promoter; the shRN…
    Available since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only

  11. pcDNA5/FRT/TO-GAlphaoA-RLuc8

    Plasmid
    #140976
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphaoA-RLuc8 (GNAO1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140976-plasmid-map-sequence-id-300659

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  16. pcDNA5/FRT/TO-GAlpha15-RLuc8

    Plasmid
    #140984
    Purpose
    Encodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlpha15-RLuc8 (GNA15 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140984-plasmid-map-sequence-id-300775

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  17. pSCALPs-HA-NFAT1 (4-460)-GFP

    Plasmid
    #88879
    Purpose
    Lentiviral vector expressing the fusion protein HA-NFAT1 (4-460)-GFP
    Depositor
    Silvia Monticelli
    Article
    Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi
    Insert
    HA-NFAT1 (4-460)-GFP fusion protein (Nfatc2 Mouse)
    Use
    Lentiviral
    Tags
    GFP fusion protein and HA tag
    Expression
    Mammalian
    Mutation
    amino acids 4-460 only; L78P & L287P *see bel…
    Promoter
    SFFV (spleen focus forming virus)
    Available since
    May 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    88879-plasmid-map-sequence-id-177202

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Showing: 621 - 640 of 7181 results