We narrowed to 28,505 results for: PLE

1. pLenti-v2-ALDH1A3gRNA2

   Plasmid

   #189747

   Purpose

   The construct was used for expression of gRNA and Cas9 for knocking out of the ALDH1A3 gene.

   Depositor

   Robert Sobol

   Article

   Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

   Insert

   Cas9, ALDH1A3 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-ALDH1A3gRNA3

   Plasmid

   #189748

   Purpose

   The construct was used for expression of gRNA for knocking out of the ALDH1A3 gene in Cas9 expressing cells.

   Depositor

   Robert Sobol

   Article

   Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

   Insert

   ALDH1A3 gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-v2-ALDH1A3gRNA1

   Plasmid

   #189746

   Purpose

   The construct was used for expression of gRNA and Cas9 for knocking out of the ALDH1A3 gene.

   Depositor

   Robert Sobol

   Article

   Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

   Insert

   Cas9, ALDH1A3 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBS Coupler 6

   Plasmid

   #185829

   Purpose

   Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

   Depositor

   Atsushi Miyawaki

   Article

   Shimozono et al Methods Cell Biol. 2008;85:381-93. doi:

   Insert

   (GlyGlyGlyGlySer)x3 linker
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBS Coupler 5

   Plasmid

   #185828

   Purpose

   Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

   Depositor

   Atsushi Miyawaki

   Article

   Shimozono et al Methods Cell Biol. 2008;85:381-93. doi:

   Insert

   (GlyGlyGlyGlySer)x3 linker
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBS Coupler 4

   Plasmid

   #185827

   Purpose

   Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

   Depositor

   Atsushi Miyawaki

   Article

   Shimozono et al Methods Cell Biol. 2008;85:381-93. doi:

   Insert

   (GlyGlyGlyGlySer)x3 linker
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBS Coupler 2

   Plasmid

   #185825

   Purpose

   Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

   Depositor

   Atsushi Miyawaki

   Article

   Shimozono et al Methods Cell Biol. 2008;85:381-93. doi:

   Insert

   (GlyGlyGlyGlySer)x3 linker
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBS Coupler 3

   Plasmid

   #185826

   Purpose

   Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

   Depositor

   Atsushi Miyawaki

   Article

   Shimozono et al Methods Cell Biol. 2008;85:381-93. doi:

   Insert

   (GlyGlyGlyGlySer)x3 linker
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLEX307_PDHA2S291D/S293D

   Plasmid

   #115195

   Purpose

   Lentiviral transduction and expression of PDHA2S291D/S293D into any mammalian cell

   Depositor

   Mohan Babu

   Article

   Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

   Insert

   PDHA2S291D/S293D (PDHA2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   p.S291D/S293D

   Promoter

   EF1alpha

   Available since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLEX307_CR-PDHA2S291D

    Plasmid

    #115200

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

    Depositor

    Mohan Babu

    Article

    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

    Insert

    CR (CRISPR/Cas9-resistant)-PDHA2S291D (PDHA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    c.321C>T (silent when translated to protein, c…

    Promoter

    EF1alpha

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEX307_CR-PDHA2S293D

    Plasmid

    #115201

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

    Depositor

    Mohan Babu

    Article

    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

    Insert

    CR (CRISPR/Cas9-resistant)-PDHA2S293D (PDHA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    c.321C>T (silent when translated to protein, c…

    Promoter

    EF1alpha

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCJH026_proD_Cas12c-multiplexing_CAM

    Plasmid

    #183073

    Purpose

    Plasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repression

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Expressing two Cas12c sgRNAs (targeting GFP or RFP) in one transcript
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLeGO.sgGata1.4.RUNX1A.iG2

    Plasmid

    #181976

    Purpose

    gene knock out of mouse GATA1, overexpression of 3xFLAG-RUNX1A (human)

    Depositor

    Jan-Henning Klusmann

    Inserts

    GATA1 gRNA: CCTAGACCAGGAAAATCCAT (Gata1 Mouse)
    RUNX1A (RUNX1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mEYFP-(L)-mApple

    Plasmid

    #182860

    Purpose

    Hetero-dimer expression vector

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

    Insert

    mEYFP-mApple
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mEGFP-(L)-mApple

    Plasmid

    #182856

    Purpose

    Hetero-dimer expression vector

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

    Insert

    mEGFP-mApple
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLentiCRISPRv2-POLB-KO-g1

    Plasmid

    #176090

    Purpose

    Cas9 plus POLB gRNA #1; contains a puromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    POLB sgRNA #1 (POLB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLentiguide-puro_hNKD2sg#3

    Plasmid

    #174325

    Purpose

    gRNA targeting hNKD2 - encodes puromycin resistance

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    human NKD2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLentiguide-puro_hNKD2sg#2

    Plasmid

    #174324

    Purpose

    gRNA targeting hNKD2 - encodes puromycin resistance

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    human NKD2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLentiguide-puro_hNKD2sg#1

    Plasmid

    #174323

    Purpose

    gRNA targeting hNKD2 - encodes puromycin resistance

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    human NKD2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC19-pleCopyCatcher

    Plasmid

    #174061

    Purpose

    CopyCatcher insertion donor for the pale locus

    Depositor

    Ethan Bier

    Article

    Li et al Nat Commun. 2021 May 11;12(1):2625. doi:

    Insert

    Pale (Pale Fly, Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge