Addgene

Showing: 621 - 640 of 1352 results

Results per page:

Sort results by:

CHI +N122D

Plasmid

#187427

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al bioRxiv 2021.11.29.470343

Inserts

ATP1A1
ATP1B1

Use

Tags

Expression

Insect

Mutation

changed asparagine at 130 to aspartic acid

Promoter

PH and p10

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP.N3-myc-SLC35A2

Plasmid

#186285

Purpose

transient expression of N-terminal myc tagged SLC35A2 isoform c/UGT1 in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Li et al Glycobiology. 2019 Jun 1;29(6):490-503.

Insert

SLC35A2 (SLC35A2 Human)

Use

Tags

MYC

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AP-VAMP7(low)

Plasmid

#167998

Purpose

positive selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

VAMP7(K121-R180)-T7PAP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-CV-VAMP1

Plasmid

#168022

Purpose

Expresses eCFP-VAMP1(T29-K89)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-VAMP1(T29-K89)-mVenus

Use

Tags

Hisx6

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-CV-VAMP4

Plasmid

#168025

Purpose

Expresses eCFP-VAMP4(P48-R107)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-VAMP4(P48-R107)-mVenus

Use

Tags

Hisx6

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-CV-VAMP3

Plasmid

#168024

Purpose

Expresses eCFP-VAMP3(S11-R69)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-VAMP3(S11-R69)-mVenus

Use

Tags

Hisx6

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-CV-VAMP7

Plasmid

#168027

Purpose

Expresses eCFP-VAMP7(K121-R180)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-VAMP7(K121-R180)-mVenus

Use

Tags

Hisx6

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-CV-VAMP5

Plasmid

#168026

Purpose

Expresses eCFP-VAMP5(M1-Q60)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-VAMP5(M1-Q60)-mVenus

Use

Tags

Hisx6

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pXW101Plux2-Pecf11-gfp

Plasmid

#160823

Purpose

Two-layered AHL-responsive circuit carrying an ECF11-based amplifier

Depositor

Baojun Wang

Article

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

PJ23101-30luxR-t-Plux2-33ecf11-Pecf11-30gfp-t

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PJ23101, Plux2, Pecf11

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SNV-memTdTomato-2AcytoGFP

Plasmid

#53124

Purpose

Retrovirus vector backbone that co-expresses GFP and a gene of interest fused to memTdTomato, driven by the Spleen Necrosis Virus promoter; for use in chicken cells

Depositor

Jeanette Hyer

Article

Venters et al Dev Dyn. 2008 Nov;237(11):3260-9. doi: 1

Type

Empty backbone

Use

Retroviral; Expression in chicken cells

Tags

2A-cyto-GFP and memTdTomato

Expression

Mutation

Promoter

SNV (spleen necrosis virus)

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
SNV-memTdTomato

Plasmid

#53123

Purpose

retroviral vector expressing membrane TdTomato, driven by the Spleen Necrosis Virus promoter; for use in chicken cells

Depositor

Jeanette Hyer

Article

Venters et al Dev Dyn. 2008 Nov;237(11):3260-9. doi: 1

Insert

memTdTomato

Use

Retroviral; Expression in chicken cells

Tags

Expression

Mutation

Promoter

SNV (spleen necrosis virus)

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
SNV-lacZ

Plasmid

#53125

Purpose

retroviral vector expressing lacZ, driven by the Spleen Necrosis Virus promoter; for use in chicken cells

Depositor

Jeanette Hyer

Article

Venters et al Dev Dyn. 2008 Nov;237(11):3260-9. doi: 1

Insert

lacZ (lacZ E.coli)

Use

Retroviral; Expression in chicken cells

Tags

Expression

Mutation

Promoter

SNV (spleen necrosis virus)

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pMA3228

Plasmid

#46856

Purpose

A lentiviral vector encoding a fusion between codon-optimized P.aeruginosa exoY mutant (K81M) and myc-tagged destabilized FKBP12

Depositor

Mikhail Alexeyev, Troy Stevens

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Insert

ExoY K81M

Use

Lentiviral

Tags

FKBP E31G, R71G, K105E and Myc

Expression

Mutation

Promoter

TRE-Tight

Available since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Flag-Tipin-E185A

Plasmid

#23114

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 Mar 15. ():.

Insert

Tipin (TIPIN Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed Glutamate 185 to Alanine

Promoter

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-Flag-Tipin-E190A

Plasmid

#23115

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 Mar 15. ():.

Insert

Tipin (TIPIN Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed Glutamate 190 to Alanine

Promoter

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pET21b-Tipin-His-L195A

Plasmid

#23123

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 Mar 15. ():.

Insert

Tipin (TIPIN Human)

Use

Tags

His

Expression

Bacterial

Mutation

Changed Leucine 195 to Alanine

Promoter

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
AIRAP 5: AIRAP(1-50).GST,V1.pcDNA3

Plasmid

#21795

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

AIRAP (Zfand2a Mouse)

Use

Tags

GST

Expression

Mammalian

Mutation

amino acids 1-50

Promoter

Available since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416 Gal-RNQ1 L94A-YFP

Plasmid

#18692

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 L94A (RNQ1 Budding Yeast)

Use

Tags

YFP

Expression

Yeast

Mutation

Leucine 94 mutated to Alanine (L94A)

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pAB042a

Plasmid

#79192

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 gIII, luxAB; Ptet rpoZ-YSX1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Plac62; Ptet

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB060c

Plasmid

#79196

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 luxAB; Ppro1 lambda cI-SH2ABL1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Plac62; Ppro1

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge