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pLenti PGK Puro GFP-GABARAPL1 G116

Plasmid

#123243

Purpose

Expression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116. For lentivirus production and stable transduction in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Deleted amino acid 117. Stop codon after G116

Promoter

PGK

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLex 983-Micu2

Plasmid

#50372

Purpose

Expression in mammalian cells

Depositor

Vamsi Mootha

Article

Plovanich et al PLoS One. 2013;8(2):e55785. doi: 10.1371

Insert

MICU2 (MICU2 Human)

Use

Lentivirus

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only
pLenti-BE4GamRA-P2A-Puro

Plasmid

#112673

Purpose

Lentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE4GamRA

Use

Lentiviral

Tags

FLAG

Expression

Mutation

NLS sequence at the N-terminus and D10A

Promoter

EF1s

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-hXRCC1-mCherry-Hygro

Plasmid

#176532

Purpose

mCherry fused to the C-terminus of XRCC1 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Wilk et al Sci Rep. 2020 Jan 20;10(1):651. doi: 10.

Insert

X-Ray Repair Cross Complementing 1 (XRCC1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-PLXNB2

Plasmid

#86237

Purpose

Lentivirus for expression of human Plexin-B2

Depositor

Roland Friedel

Article

Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

Insert

Plexin-B2 (PLXNB2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

full length PLXNB2, CRISPR resistant

Promoter

human PGK promoter

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-BE4RA-P2A-Puro

Plasmid

#112672

Purpose

Lentivirus for constitutive expression of BE4RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE4RA

Use

Lentiviral

Tags

FLAG

Expression

Mutation

NLS sequence at the N-terminus and D10A

Promoter

EF1s

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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Luciferase-pcw107

Plasmid

#64648

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

n/a

Use

Lentiviral

Tags

Expression

Mutation

Promoter

PGK

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pSLIK-NFLAG-hFUS

Plasmid

#50487

Purpose

Produces lentivirus expressing human FUS with FLAG tag at its N-terminus by co-transfection with psPAX2 and pMD2G

Depositor

Zissimos Mourelatos

Article

Nakaya et al RNA. 2013 Apr;19(4):498-509. doi: 10.126

Insert

FUS (FUS Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

TRE

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only
pHAGE2-TetO-hSOX17

Plasmid

#206389

Purpose

Expresses human SOX17 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

Insert

SOX17 (SOX17 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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HcRed-pcw107-V5

Plasmid

#64647

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

n/a

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

PGK

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pLenti-LmoCas8b2

Plasmid

#126488

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas8b2 driven by hUbC promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas8b2

Use

CRISPR and Lentiviral

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-LmoCas7

Plasmid

#126489

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas7 driven by hUbC promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas7

Use

CRISPR and Lentiviral

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-LmoCas5

Plasmid

#126490

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas5 driven by hUbC promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas5

Use

CRISPR and Lentiviral

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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plentiCRISPRv2-sgPLXNB2

Plasmid

#86152

Purpose

Lentivirus carrying Cas9/CRISPR for cut in human PLXNB2 gene exon 3

Depositor

Roland Friedel

Article

Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

Insert

Plexin-B2 (PLXNB2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-LmoCas6

Plasmid

#126491

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6 driven by hUbC promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas6

Use

CRISPR and Lentiviral

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL1401 pLenti-pEF1a(short)-mNeonGreen-P2A-Cas9

Plasmid

#163644

Purpose

Makes a lentivirus that expresses mNeonGreen and Cas9.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

mNeonGreen-P2A-Cas9

Use

Lentiviral

Tags

mNeonGreen-P2A

Expression

Mutation

Promoter

EFS

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pOUPc-UL148-HA

Plasmid

#179343

Purpose

All-in-one "tet-on" 3G lentiviral vector plasmid encoding human cytomegalovirus UL148 ER resident glycoprotein with a C-terminal HA tag fused to its cytoplasmic tail

Depositor

Jeremy Kamil

Article

Siddiquey et al J Virol. 2018 Sep 26;92(20). pii: JVI.00

Insert

human cytomegalovirus UL148 fused to HA epitope

Use

Lentiviral and Synthetic Biology; Hiv-1 based rep…

Tags

HA tag

Expression

Mammalian

Mutation

insert matches UL148 from strain TB40/E

Promoter

Tet responsive element 3G

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-EGFP-PolB-Hygro

Plasmid

#176056

Purpose

EGFP fused to the N-terminus of PolB & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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GP804

Plasmid

#164548

Purpose

Expresses Smo-FLAG K543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

All sixteen Lysines in cytoplasmic C-terminal tai…

Promoter

GgCryD1

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-LmoCas6-KRAB-2a-BlastR

Plasmid

#126492

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6-KRAB-2a-BlastR driven by hUbC promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas6-KRAB-2a-BlastR

Use

CRISPR and Lentiviral

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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